NEWHELIX, JUNE 1989 EDITION 22 June 1989 This NEWHEL tape contains the NEWHELIX.FOR parameter calculating program, along with NEWHELIX.COM, which serves as an input control parameter file, an example of an input coordinate list NEWTEST.INP, and the resulting output tables, NEWTEST.OUT. The example, C-G-C-G-C-G-T-T-T-T-C-G-C-G-C-G, illustrates the ability of NEWHELIX to handle non-selfcomplementary sequences and non-(purine/pyrimidine) mismatches (the two "TT pairs"). If you received a mid-April copy of NEWHELIX.FOR, discard it, as it was restricted to purine/pyrimidine pairs only and gave the wrong sign for Y DSP. The May edition corrected these signs. The present June edition calculates two useful new functions. In the torsion angle tables, the quantity (epsilon-zeta) now is listed in the right column. In the cylin- drical tables, the little-used R(P) column has been replaced by the distance between successive C1' atoms along one chain, measured in projection on a plane perpendicular to the helix axis. This is a useful measure of base overlap from one base to the next along a strand. You should also have been sent a hard-copy manual: "NEWHELIX INSTRUCTIONS", consisting of 10 pages of instructions and several pages of examples of input/output. If you do not have a copy, contact: Richard E. Dickerson Molecular Biology Institute U. C. L. A. Los Angeles CA 90024, U.S.A. (213)825-5864 NEWHELIX now is consistent with the September 1988 Cambridge Conventions on definitions and nomenclature. I have also added to this tape the four programs of the old HELIB package: AHELIX, BROLL, CYLIN and DTORAN, also brought up to the Cambridge standards. NEWHELIX is the program of choice for all double-helical applications. But the more flexible (if more tedious) input format of the HELIB programs can be of value in special circumstances.