2011-04-26 REL 2009-06-22 2011-04-26 2011-04-26 8-A cryoEM structure and molecular model of the myriapod (Scutigera) 6x6mer hemocyanin: understanding a giant oxygen transport protein J.Markl,A.Moeller,A.G.Martin,J.Rheinbay,W.Gebauer,F.Depoix hemocyanin, 3D electron microscopy, molecular model, allostery, Myriapoda Markl J, Moeller A, Martin AG, Rheinbay J, Gebauer W, Depoix F 10-A cryoEM structure and molecular model of the Myriapod (Scutigera) 6x6mer hemocyanin: understanding a giant oxygen transport protein. J.MOL.BIOL. 392 362 380 2009 19591844 doi:10.1016/j.jmb.2009.06.082 EMD-1628.map Image stored as Reals 340 340 340 0 0 0 339 339 339 340 340 340 340 340 340 90 90 90 X Y Z -0.0127973 0.0186785 0.00013993 0.00129549 1
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1 1 1 0.0012 Hemocyanin from the myriapod Scutigera 1 singleParticle Scutigera hemocyanin 6x6mer 2.7 protein Scutigera hemocyanin ScoHc 2.7 Scutigera coleoptrata false 6x6mer Hemolymph ETHANE
Vitrification instrument: Homebuilt
90 HOMEMADE PLUNGER FIELD EMISSION GUN DARK FIELD SPOT SCAN ST cryo holder FEI TECNAI F30 GATAN LIQUID NITROGEN 300
50mM Tris/HCl, 150mM NaCl
1 7.4 cryo EM, no stain 400 mesh copper IMAGIC-5 CTFFIND3 and transfer IMAGIC-5 8 FSC-half bit 6484