2011-08-31 REL 2007-06-25 PDBe PDBe 2007-06-25 2007-06-25 Averaging tens to hundreds of icosahedral particle images to resolve protein secondary structure elements using a Multi-Path Simulated Annealing optimization algorithm. Liu X, Jiang W, Jakana J, Chiu W Liu X, Jiang W, Jakana J, Chiu W Averaging tens to hundreds of icosahedral particle images to resolve protein secondary structure elements using a Multi-Path Simulated Annealing optimization algorithm. J.STRUCT.BIOL. 160 11 27 2007 17698370 doi:10.1016/j.jsb.2007.06.009 emd_1376.map.gz Image stored as Reals 128 128 128 -64 -64 -64 63 63 63 128 128 128 190.6 190.6 190.6 90 90 90 X Y Z -0.571971 0.718931 0.00185874 0.0279092 1
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1.48906 1.48906 1.48906 0.0298 This is a P3A subunit of a RDV (Rice Dwarf Virus) map. It was reconstructed from the best 284 particles. For the full map, see accession code: EMD-1375. Rice Dwarf Virus 1 50 virus Rice dwarf virus Rice dwarf virus false false OTHER OTHER PLANTAE(HIGHER PLANTS) 550 Inner Shell 1 ETHANE 27 111 JEOL 4000 Gatan GATAN LIQUID NITROGEN 1999-01-01 400 FLOOD BEAM BRIGHT FIELD 4.1 300 1600 50000 49495 LAB6 13 109 KODAK SO-163 FILM
Microscope:JEOL 4000
 100 4 32 OTHER particle singleParticle Cross Common Lines Multi-path SA Each particle 7.9 FSC 0.5
The 5 alignment parameters (2 for center and 3 for orientation) were searched simultaneously for each particle image. The search is executed by a new optimizaiton algorithm, multi-path simulated annealing, based on Cross Common Lines. There were 4200 good particles discriminated from 4865 raw particles by a Consistency Criterion. The best 284 particles were empirically selected by a double threshold method from the 4200 good particles. The map's resolution was measured against the Known X-ray structure (PDB ID:1UF2).
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