2017-12-06 HPUB 2017-09-27 PDBe PDBe 2017-10-04 YaxAB pore complex Braeuning B, Bertosin E, Dietz H, Groll M 6el1 Braeuning B, Bertosin E, Praetorius F, Ihling C, Schatt A, Adler A, Richter K, Sinz A, Dietz H, Groll M Structure and mechanism of the two-component alpha-helical pore-forming toxin YaxAB. to be published 0353 emd_3885.map.gz Image stored as Reals 400 400 400 0 0 0 399 399 399 400 400 400 442.39996 442.39996 442.39996 90.0 90.0 90.0 X Y Z -0.02732106 0.14460759 0.00082460826 0.0069432124 1
1.106 1.106 1.106 0.045 3 YaxAB complex (10x YaxA + 10x YaxB)
Purified with Cymal-6 detergent and reconstituted in amphipol prior to Cryo-EM.
0.85 protein YaxAB complex (10x YaxA + 10x YaxB) 0.85 Yersinia enterocolitica false Escherichia coli
Purified with Cymal-6 detergent and reconstituted in amphipol prior to Cryo-EM.
 protein Uncharacterized protein 0.045877156 false Yersinia enterocolitica protein YaxB 0.039248836 false Yersinia enterocolitica ETHANE 100.0 20.0 FEI VITROBOT MARK IV
3 mM F-FOS Choline 8 just before vitrification blot for 3 to 4 s blot distance -2 to -1 mm
 FIELD EMISSION GUN 60.0 BRIGHT FIELD FLOOD BEAM OTHER FEI TITAN KRIOS OTHER 300 6EK7 flexible REAL particle 2 7. singleParticle RELION 5.5 FSC 0.143 CUT-OFF C10 25000