Signaling in Chemoreceptor Arrays Through Mobility Control of Kinase Domains - low kinase activity state

2013-09-11 REL 2012-12-14 RCSB RCSB 2012-12-26 2013-07-17 EMD-5541, EMD-5543, EMD-5545, EMD-5546, EMD-5547, EMD-5548, EMD-5549, EMD-5550 Signaling in Chemoreceptor Arrays Through Mobility Control of Kinase Domains - low kinase activity state - tsr mutant P221D Briegel A, Ames P, Gumbart JC, Oikonomou CM, Parkinson JS, Jensen GJ chemotaxis, E.coli, CheA Briegel A, Ames P, Gumbart JC, Oikonomou CM, Parkinson JS, Jensen GJ The mobility of two kinase domains in the Escherichia coli chemoreceptor array varies with signalling state. MOL.MICROBIOL. 89 831 841 2013 23802570 doi:10.1111/mmi.12309 emd_5542.map.gz Image stored as Reals 60 60 60 0 0 0 59 59 59 60 60 60 386.4 386.4 386.4 90.0 90.0 90.0 X Y Z 0E-8 0.36071506 0.22182390 0.08268128 1

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6.44 6.44 6.44 0.275 Subvolume average of mutant receptor tsrP221D/CheA/CheW 3 chemoreceptor array with mutant receptor tsr P221D protein tsr P221D Escherichia coli true Escherichia coli protein CheW false Escherichia coli protein CheA dimer Escherichia coli false ETHANE-PROPANE MIXTURE 100 FEI VITROBOT MARK III FIELD EMISSION GUN 150 FEI BRIGHT FIELD 10000 FLOOD BEAM 2.2 -66.93 59.52 FEI POLARA 300 18-AUG-2011 OTHER 300 34000 0-20 R 2/2 copper/rhodium grids, glow discharged

Tryptone Broth (10 g/L Tryptone, 5 g/L NaCl). Cells were incubated with penicillin for 1 hour prior to freezing.

subtomogramAveraging IMOD, tomo3D IMOD 25

CTF-corrected. Average number of tilts used in the 3D reconstructions: 128. Average tomographic tilt angle increment: 1.