2013-06-05 REL 2013-03-21 RCSB RCSB 2013-04-03 2013-05-15 Yeast 20S proteasome with C-terminal peptide of yeast Rpt4 Li X, Kim HM, Cheng Y Yeast 20S proteasome, C-terminal peptide, yeast Rpt4 Park S, Li X, Kim HM, Singh CR, Tian G, Hoyt MA, Lovell S, Battaile KP, Zolkiewski M, Coffino P, Roelofs J, Cheng Y, Finley D Reconfiguration of the proteasome during chaperone-mediated assembly. NATURE 497 512 516 2013 23644457 doi:10.1038/nature12123 emd_5614.map.gz Image stored as Reals 200 200 200 0 0 0 199 199 199 200 200 200 294.0 294.0 294.0 90.0 90.0 90.0 X Y Z -1.19386077 2.49575472 -0.02869725 0.17732443 1
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1.47 1.47 1.47 0.8 3D density map of yeast 20S proteasome core particle with GST tag added to the C-terminus of beta-2 subunit in complex with the C-terminal peptide of yeast Rpt4 1 Yeast 20S proteasome 28mer 0.7 protein 20S proteasome 0.7
GST tag was added to C-terminus of beta-2 subunit
Saccharomyces cerevisiae false Baker's yeast ETHANE 100 FEI VITROBOT MARK III 120 FIELD EMISSION GUN 25 BRIGHT FIELD 1200 2100 FLOOD BEAM CT3500
Low-Dose procedure
TVIPS TEMCAM-F816 (8k x 8k) 2.0 0 0 FEI TECNAI F20 01-JAN-2010 GATAN LIQUID NITROGEN 200 100000 Chimera rigid body REAL 1RYP particle Quantifoil Grid singleParticle FREALIGN Each particle 7.8 FSC 0.143 C2 36303
FREALIGN