2013-12-11 REL 2013-09-27 RCSB RCSB 2013-10-23 2013-12-11 Hepatitis C Virus E2 Envelope Glycoprotein Core Structure Nieusma T, Kong L, Giang E, Kadam RU, Cogburn KE, Hua Y, Dai X, Stanfield RL, Burton DR, Wilson IA, Law M, Ward AB hepatitis C, virus, envelope, glycoprotein Kong L, Giang E, Nieusma T, Kadam RU, Cogburn KE, Hua Y, Dai X, Stanfield RL, Burton DR, Ward AB, Wilson IA, Law M Hepatitis C virus E2 envelope glycoprotein core structure. SCIENCE 342 1090 1094 2013 24288331 doi:10.1126/science.1243876 emd_5760.map.gz Image stored as Reals 96 96 96 -23 -23 -23 72 72 72 96 96 96 196.79999 196.79999 196.79999 90.0 90.0 90.0 X Y Z -5.24226284 10.52967072 0.00000713 0.80545229 1
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2.05 2.05 2.05 1.84 E2 glycoprotein bound to AR3A Fab and AR2A Fab, single particle reconstruction 3 E2 glycoprotein bound to AR3A Fab and AR2A Fab 0.146 protein E2 envelope glycoprotein E2 0.046 Hepatitis C virus 1 true monomer pCMV-Tag4A-tpa Homo sapiens HEK 293T protein Antigen-binding fragment of antibody AR3A 0.050 true Homo sapiens monomer 1 Human BL21(DE3) Escherichia coli pComb3H protein Antigen-binding fragment of antibody AR2A 0.050 Homo sapiens 1 Human monomer true pComb3H Escherichia coli BL21(DE3) NONE NONE LAB6 BRIGHT FIELD 1000 1000 FLOOD BEAM TVIPS TEMCAM-F416 (4k x 4k) 0 55 298 FEI TECNAI SPIRIT 12-JUN-2013 SIDE ENTRY, EUCENTRIC 120 52000 452 negative stain with uranyl formate particle 400 mesh copper singleParticle Initiated with common lines Appion, Spider, Xmipp, Eman1, Eman2 20 FSC 0.5 C1 27050
Particles were selected using automatic (difference-of-Gaussians) picking followed by reference-free classification to eliminate noisy picks or non-target aggregation states.