2016-02-24 REL 2015-11-15 RCSB PDBj 2015-12-09 2016-02-24 Structure and function of outer dynein intermediate and light chain complex Oda T, Abe T, Yanagisawa H, Kikkawa M cilia and flagella, axoneme, outer dynein arm, intermediate chain, light chain Oda T, Abe T, Yanagisawa H, Kikkawa M Structure and function of outer dynein arm intermediate and light chain complex MOL.BIOL.CELL n/a n/a n/a 2016 26864626 doi:10.1091/mbc.E15-10-0723 emd_6515.map.gz Image stored as Reals 150 200 170 0 0 0 149 199 169 150 200 170 910.5 1214.0 1031.9 90.0 90.0 90.0 X Y Z 0E-8 600.27740479 151.30323792 149.78021240 1
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6.07 6.07 6.07 322 Streptavidin-labeled IC1M31BCCP axoneme 1 Streptavidin-labeled IC1M31BCCP axoneme cellular-component axoneme false Chlamydomonas reinhardtii flagella Blot for 5 seconds before plunging ETHANE 90 LEICA EM GP 93 FIELD EMISSION GUN 100 Omega BRIGHT FIELD 6000 9000 FLOOD BEAM TVIPS TEMCAM-F416 (4k x 4k) 2.2 -60 60 OTHER 12-JUN-2015 GATAN LIQUID NITROGEN 300 25700 0-20 cell 0.01 300 mesh copper grid, holey carbon
30 mM Hepes-NaOH pH 7.2, 5 mM MgCl2, 1 mM dithiothreitol, 1 mM EGTA, 50 mM K-acetate
7.2 subtomogramAveraging IMOD, PEET 47 FSC 0.5, semi-independent 848 C1
Number of tilts (projections) used in 3D reconstruction: 60 Tomographic tilt angle increment: 2