2014-08-27 REL 2014-04-15 RCSB RCSB 2014-07-30 2014-07-30 Bacteriophage CUS-3 asymmetric reconstruction Parent KN, Tang J, Cardone G, Gilcrease EB, Janssen ME, Olson NH, Casjens SR, Baker TS mature virion Parent KN, Tang J, Cardone G, Gilcrease EB, Janssen ME, Olson NH, Casjens SR, Baker TS Three-dimensional reconstructions of the bacteriophage CUS-3 virion reveal a conserved coat protein I-domain but a distinct tail spike receptor-binding domain VIROLOGY 464 55 66 2014 25043589 doi:10.1016/j.virol.2014.06.017 emd_5946.map.gz Image stored as Reals 401 401 401 -200 -200 -200 200 200 200 401 401 401 656.838 656.838 656.838 90.0 90.0 90.0 X Y Z 151.03454590 852.00000000 385.11666870 68.56067657 1
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1.638 1.638 1.638 426 Bacteriophage CUS-3 asymmetric reconstruction
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1 CUS-3 virion virus Enterobacteria phage CUS-3 Enterobacteria phage CUS-3 false false SPECIES VIRION K1 Escherichia coli BACTERIA(EUBACTERIA) 690 capsid 7 Blot for 5 sec before plunging. ETHANE 100 HOMEMADE PLUNGER 90 Objective lens astigmatism was corrected at the magnification used to collect data. FIELD EMISSION GUN 23 BRIGHT FIELD 150 89 3660 FLOOD BEAM 91 KODAK SO-163 FILM 2.3 38769 90 FEI POLARA 300 01-DEC-2012 OTHER 200 39000 NIKON SUPER COOLSCAN 8000 153 6 particle 10 400 mesh R2/2 Quantifoil, glow discharged
10 mM Tris, 10 mM MgCl2
7.6 singleParticle icosahedral reconstruction, tail alignment, asymmetric averaging Auto3dem, RobEM, autopp each micrograph 20 FSC 0.5, gold-standard C1 4819
Auto3dem was used for refinement.