2018-08-22 REL 2017-05-10 PDBj PDBj 2018-06-13 2018-06-13 Folding intermediate of RuBisCO in complex with the GroEL chaperonin. Class3. Natesh R, Clare DK, Farr GW, Horwich AL, Saibil HR Natesh R, Clare DK, Farr GW, Horwich AL, Saibil HR A two-domain folding intermediate of RuBisCO in complex with the GroEL chaperonin. Int. J. Biol. Macromol. 118 671 675 2018 29959019 doi:10.1016/j.ijbiomac.2018.06.120 1879-0003 0708 IJBMDR emd_6727.map.gz Image stored as Reals 196 196 196 0. 0. 0. 195. 195. 195. 196 196 196 548.8 548.8 548.8 90.0 90.0 90.0 X Y Z -0.70907927 9.996134 0.016255789 0.23185983 1
2.8 2.8 2.8 0.25 Folding intermediate of RuBisCO in complex with the GroEL chaperonin. Class3. 2 Non-native RuBisCO in complex with chaperonin GroEL
Apo GroEL.D473C.His6 with unbound RuBisCO folding intermediate as seen in 3D reconstruction for Class3. Only GroEL region was observed in EM map.
protein Non-native RuBisCO in complex with chaperonin GroEL 0.812
Apo GroEL.D473C.His6 with unbound RuBisCO folding intermediate as seen in 3D reconstruction for Class3. Only GroEL region was observed in EM map.
Escherichia coli false Escherichia coli protein GroEL
GroEL.D473C.His6 Tetradecamer
Escherichia coli false Escherichia coli ETHANE 298 HOMEMADE PLUNGER
Blot approx. 1 sec before plunging. Visual..
FIELD EMISSION GUN 10.0 BRIGHT FIELD 1000.0 3500.0 FLOOD BEAM KODAK SO-163 FILM 2.0 50000. 100.0 100.0 FEI TECNAI F20 GATAN LIQUID NITROGEN 200 50000. 468 7.0
Images were collected on Kodak SO-163 Film. Dose was 10-15 e-/A2/sec.
 1OEL rigid body
Rigid body fit
 particle 0.0863 7.5
Folding Buffer (FB) consists of 50 mM HEPES pH7.5, 10 mM KOAc, 10 mM Mg(OAc)2, 10 mM DTT. RuBisCO denatured in 20mM HCl, 10 M Urea, 20 mM DTT
 The C-flat holey grids were coated with thin home made carbon film. singleParticle BACK PROJECTION CTF phase flipping was perfomed 11.0 FSC 0.5 CUT-OFF
In 3D reconstruction for Class 3: Resolution is 9 A for C7 symmetrised map after iteration cycle 7. Resolution is 11 A for asymmetric C1 reconstruction after iteration cycle 13.
 C1 6003
For the binary complex data sets, the positions of particles were noted in MRC programme XIMDISP. Perl script was used to extract particles in boxes of 512 x 512 pixels using the MRC program LABEL, and phase corrected using SPIDER. After CTF correction, the box size was cropped and sampling reduced so that all images were at 2.8 A per pixel in 196 x 196 pixel boxes. Images were band-pass filtered between 285 A and 6 A and normalized to zero mean and the same sigma in SPIDER.