2020-03-11 REL 2019-08-15 PDBj PDBj 2020-02-12 2020-02-12 Cryo-EM structure of CB1-G protein complex Hua T, Li XT, Wu LJ, Makriyannis A, Wang YX, Shen L, Liu ZJ 6kpg Hua T, Li X, Wu L, Iliopoulos-Tsoutsouvas C, Wang Y, Wu M, Shen L, Johnston CA, Nikas SP, Song F, Song X, Yuan S, Sun Q, Wu Y, Jiang S, Grim TW, Benchama O, Stahl EL, Zvonok N, Zhao S, Bohn LM, Makriyannis A, Liu ZJ Activation and Signaling Mechanism Revealed by Cannabinoid Receptor-GiComplex Structures. Cell 180 655 2020 32004463 doi:10.1016/j.cell.2020.01.008 1097-4172 emd_0745.map.gz Image stored as Reals 224 224 224 0 0 0 223 223 223 224 224 224 232.95999 232.95999 232.95999 90.0 90.0 90.0 X Y Z -0.11882624 0.18688558 0.000011653 0.0043318244 1
1.04 1.04 1.04 0.025 7 CB1-G protein complex protein CB1-G protein complex 140 Homo sapiens false Spodoptera frugiperda protein Guanine nucleotide-binding protein G(i) subunit alpha-1 0.040283836 Homo sapiens 1 false Spodoptera frugiperda protein Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 0.038045629 Homo sapiens 1 false Spodoptera frugiperda protein Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 0.007861143 Homo sapiens 1 false Spodoptera frugiperda protein Cannabinoid receptor 1 0.052843691 Homo sapiens 1 false Mammalian expression vector HA-MCS-pcDNA3.1 protein scFv16 0.027742861 Homo sapiens 1 false Spodoptera frugiperda ligand (6~{a}~{R},9~{R},10~{a}~{R})-9-(hydroxymethyl)-3-(8-isothiocyanato-2-methyl-octan-2-yl)-6,6-dimethyl-6~{a},7,8,9,10,10~{a}-hexahydrobenzo[c]chromen-1-ol 0.000445658 1 false ETHANE 100 FEI VITROBOT MARK IV
FIELD EMISSION GUN 1.333 GIF Quantum LS BRIGHT FIELD -1000.0 -2000.0 FLOOD BEAM GATAN K2 SUMMIT (4k x 4k) 2.7 70.0 70.0 FEI TITAN KRIOS FEI TITAN KRIOS AUTOGRID HOLDER 300 130000 5577 6N4B rigid body 72.4 REAL particle 1 7.5 singleParticle FOURIER SPACE RELION 3.0 FSC 0.143 CUT-OFF C1 505644