2020-09-09 REL 2020-03-31 RCSB RCSB 2020-07-08 2020-07-08 Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Cognate Structure II-C1) Loveland AB, Demo G, Korostelev AA 6wd5 Loveland AB, Demo G, Korostelev AA Cryo-EM of elongating ribosome with EF-Tu•GTP elucidates tRNA proofreading. Nature 584 640 645 2020 32612237 doi:10.1038/s41586-020-2447-x 1476-4687 0006 NATUAS emd_21624.map.gz Image stored as Reals 288 288 288 0 0 0 287 287 287 288 288 288 383.904 383.904 383.904 90.0 90.0 90.0 X Y Z -9.717282 18.516886 0.11332837 0.8632146 1
1.333 1.333 1.333 1.0 Map II-C1 blocfilt filtered and with B-factor 50 apply 62 Map II-C1 protein Map II-C1 Escherichia coli MRE600 false protein 50S ribosomal protein L2 0.029663244 Escherichia coli MRE600 1 false protein 50S ribosomal protein L3 0.022277535 Escherichia coli MRE600 1 false protein 50S ribosomal protein L4 0.022121566 Escherichia coli MRE600 1 false protein 50S ribosomal protein L5 0.020073234 Escherichia coli MRE600 1 false protein 50S ribosomal protein L6 0.018801598 Escherichia coli MRE600 1 false protein 50S ribosomal protein L9 0.01578902 Escherichia coli MRE600 1 false protein 50S ribosomal protein L10 0.014112345 Escherichia coli MRE600 1 false protein 50S ribosomal protein L11 0.014763165 Escherichia coli MRE600 1 false protein 50S ribosomal protein L13 0.016050606 Escherichia coli MRE600 1 false protein 50S ribosomal protein L14 0.01345191 Escherichia coli MRE600 1 false protein 50S ribosomal protein L15 0.014877273 Escherichia coli MRE600 1 false protein 50S ribosomal protein L16 0.015312269 Escherichia coli MRE600 1 false protein 50S ribosomal protein L17 0.013721938 Escherichia coli MRE600 1 false protein 50S ribosomal protein L18 0.012663471 Escherichia coli MRE600 1 false protein 50S ribosomal protein L19 0.013028082 Escherichia coli MRE600 1 false protein 50S ribosomal protein L20 0.013396828 Escherichia coli MRE600 1 false protein 50S ribosomal protein L21 0.011586374 Escherichia coli MRE600 1 false protein 50S ribosomal protein L22 0.012253359 Escherichia coli MRE600 1 false protein 50S ribosomal protein L23 0.010546472 Escherichia coli MRE600 1 false protein 50S ribosomal protein L24 0.011078874 Escherichia coli MRE600 1 false protein 50S ribosomal protein L25 0.010713465 Escherichia coli MRE600 1 false protein 50S ribosomal protein L27 0.008174394 Escherichia coli MRE600 1 false protein 50S ribosomal protein L28 0.008896354 Escherichia coli MRE600 1 false protein 50S ribosomal protein L29 0.007286464 Escherichia coli MRE600 1 false protein 50S ribosomal protein L30 0.006423625 Escherichia coli MRE600 1 false protein 50S ribosomal protein L32 0.006332249 Escherichia coli MRE600 1 false protein 50S ribosomal protein L33 0.005814842 Escherichia coli MRE600 1 false protein 50S ribosomal protein L34 0.005397463 Escherichia coli MRE600 1 false protein 50S ribosomal protein L35 0.007181835 Escherichia coli MRE600 1 false protein 50S ribosomal protein L36 0.00437739 Escherichia coli MRE600 1 false protein 30S ribosomal protein S2 0.025015816 Escherichia coli MRE600 1 false protein 30S ribosomal protein S3 0.023078785 Escherichia coli MRE600 1 false protein 30S ribosomal protein S4 0.023383002 Escherichia coli MRE600 1 false protein 30S ribosomal protein S5 0.016532088 Escherichia coli MRE600 1 false protein 30S ribosomal protein S6 0.011669371 Escherichia coli MRE600 1 false protein 30S ribosomal protein S7 0.016861523 Escherichia coli MRE600 1 false protein 30S ribosomal protein S8 0.014015361 Escherichia coli MRE600 1 false protein 30S ribosomal protein S9 0.014554882 Escherichia coli MRE600 1 false protein 30S ribosomal protein S10 0.011196988 Escherichia coli MRE600 1 false protein 30S ribosomal protein S11 0.012388068 Escherichia coli MRE600 1 false protein 30S ribosomal protein S12 0.013636961 Escherichia coli MRE600 1 false protein 30S ribosomal protein S13 0.012625753 Escherichia coli MRE600 1 false protein 30S ribosomal protein S14 0.011475364 Escherichia coli MRE600 1 false protein 30S ribosomal protein S15 0.010159621 Escherichia coli MRE600 1 false protein 30S ribosomal protein S16 0.009207572 Escherichia coli MRE600 1 false protein 30S ribosomal protein S17 0.009263946 Escherichia coli MRE600 1 false protein 30S ribosomal protein S18 0.007606768 Escherichia coli MRE600 1 false protein 30S ribosomal protein S19 0.009057626 Escherichia coli MRE600 1 false protein 30S ribosomal protein S20 0.00950619 Escherichia coli MRE600 1 false protein 30S ribosomal protein S21 0.007763073 Escherichia coli MRE600 1 false protein 50S ribosomal protein L1 0.023735498 Escherichia coli MRE600 1 false nucleic-acid 16S ribosomal RNA 0.498725406 Escherichia coli MRE600 false AAUUGAAGAGUUUGAUCAUGGCUCAGAUUGAACGCUGGCGGCAGGCCUAACACAUGCAAGUCGAACGGUAACAGGAAGAA GCUUGCUUCUUUGCUGACGAGUGGCGGACGGGUGAGUAAUGUCUGGGAAACUGCCUGAUGGAGGGGGAUAACUACUGGAA ACGGUAGCUAAUACCGCAUAACGUCGCAAGACCAAAGAGGGGGACCUUCGGGCCUCUUGCCAUCGGAUGUGCCCAGAUGG GAUUAGCUAGUAGGUGGGGUAACGGCUCACCUAGGCGACGAUCCCUAGCUGGUCUGAGAGGAUGACCAGCCACACUGGAA CUGAGACACGGUCCAGACUCCUACGGGAGGCAGCAGUGGGGAAUAUUGCACAAUGGGCGCAAGCCUGAUGCAGCCAUGCC GCGUGUAUGAAGAAGGCCUUCGGGUUGUAAAGUACUUUCAGCGGGGAGGAAGGGAGUAAAGUUAAUACCUUUGCUCAUUG ACGUUACCCGCAGAAGAAGCACCGGCUAACUCCGUGCCAGCAGCCGCGGUAAUACGGAGGGUGCAAGCGUUAAUCGGAAU UACUGGGCGUAAAGCGCACGCAGGCGGUUUGUUAAGUCAGAUGUGAAAUCCCCGGGCUCAACCUGGGAACUGCAUCUGAU ACUGGCAAGCUUGAGUCUCGUAGAGGGGGGUAGAAUUCCAGGUGUAGCGGUGAAAUGCGUAGAGAUCUGGAGGAAUACCG GUGGCGAAGGCGGCCCCCUGGACGAAGACUGACGCUCAGGUGCGAAAGCGUGGGGAGCAAACAGGAUUAGAUACCCUGGU AGUCCACGCCGUAAACGAUGUCGACUUGGAGGUUGUGCCCUUGAGGCGUGGCUUCCGGAGCUAACGCGUUAAGUCGACCG CCUGGGGAGUACGGCCGCAAGGUUAAAACUCAAAUGAAUUGACGGGGGCCCGCACAAGCGGUGGAGCAUGUGGUUUAAUU CGAUGCAACGCGAAGAACCUUACCUGGUCUUGACAUCCACGGAAGUUUUCAGAGAUGAGAAUGUGCCUUCGGGAACCGUG AGACAGGUGCUGCAUGGCUGUCGUCAGCUCGUGUUGUGAAAUGUUGGGUUAAGUCCCGCAACGAGCGCAACCCUUAUCCU UUGUUGCCAGCGGUCCGGCCGGGAACUCAAAGGAGACUGCCAGUGAUAAACUGGAGGAAGGUGGGGAUGACGUCAAGUCA UCAUGGCCCUUACGACCAGGGCUACACACGUGCUACAAUGGCGCAUACAAAGAGAAGCGACCUCGCGAGAGCAAGCGGAC CUCAUAAAGUGCGUCGUAGUCCGGAUUGGAGUCUGCAACUCGACUCCAUGAAGUCGGAAUCGCUAGUAAUCGUGGAUCAG AAUGCCACGGUGAAUACGUUCCCGGGCCUUGUACACACCGCCCGUCACACCAUGGGAGUGGGUUGCAAAAGAAGUAGGUA GCUUAACCUUCGGGAGGGCGCUUACCACUUUGUGAUUCAUGACUGGGGUGAAGUCGUAACAAGGUAACCGUAGGGGAACC UGCGGUUGGAUCACCUCCU RNA OTHER nucleic-acid 23S ribosomal RNA 0.94130525 Escherichia coli MRE600 false GGUUAAGCGACUAAGCGUACACGGUGGAUGCCCUGGCAGUCAGAGGCGAUGAAGGACGUGCUAAUCUGCGAUAAGCGUCG GUAAGGUGAUAUGAACCGUUAUAACCGGCGAUUUCCGAAUGGGGAAACCCAGUGUGUUUCGACACACUAUCAUUAACUGA AUCCAUAGGUUAAUGAGGCGAACCGGGGGAACUGAAACAUCUAAGUACCCCGAGGAAAAGAAAUCAACCGAGAUUCCCCC AGUAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCUGAAUCAGUGUGUGUGUUAGUGGAAGCGUCUGGAAAGGCGCGCGA UACAGGGUGACAGCCCCGUACACAAAAAUGCACAUGCUGUGAGCUCGAUGAGUAGGGCGGGACACGUGGUAUCCUGUCUG AAUAUGGGGGGACCAUCCUCCAAGGCUAAAUACUCCUGACUGACCGAUAGUGAACCAGUACCGUGAGGGAAAGGCGAAAA GAACCCCGGCGAGGGGAGUGAAAAAGAACCUGAAACCGUGUACGUACAAGCAGUGGGAGCACGCUUAGGCGUGUGACUGC GUACCUUUUGUAUAAUGGGUCAGCGACUUAUAUUCUGUAGCAAGGUUAACCGAAUAGGGGAGCCGAAGGGAAACCGAGUC UUAACUGGGCGUUAAGUUGCAGGGUAUAGACCCGAAACCCGGUGAUCUAGCCAUGGGCAGGUUGAAGGUUGGGUAACACU AACUGGAGGACCGAACCGACUAAUGUCGAAAAAUUAGCGGAUGACUUGUGGCUGGGGGUGAAAGGCCAAUCAAACCGGGA GAUAGCUGGUUCUCCCCGAAAGCUAUUUAGGUAGCGCCUCGUGAAUUCAUCUCCGGGGGUAGAGCACUGUUUCGGCAAGG GGGUCAUCCCGACUUACCAACCCGAUGCAAACUGCGAAUACCGGAGAAUGUUAUCACGGGAGACACACGGCGGGUGCUAA CGUCCGUCGUGAAGAGGGAAACAACCCAGACCGCCAGCUAAGGUCCCAAAGUCAUGGUUAAGUGGGAAACGAUGUGGGAA GGCCCAGACAGCCAGGAUGUUGGCUUAGAAGCAGCCAUCAUUUAAAGAAAGCGUAAUAGCUCACUGGUCGAGUCGGCCUG CGCGGAAGAUGUAACGGGGCUAAACCAUGCACCGAAGCUGCGGCAGCGACGCUUAUGCGUUGUUGGGUAGGGGAGCGUUC UGUAAGCCUGCGAAGGUGUGCUGUGAGGCAUGCUGGAGGUAUCAGAAGUGCGAAUGCUGACAUAAGUAACGAUAAAGCGG GUGAAAAGCCCGCUCGCCGGAAGACCAAGGGUUCCUGUCCAACGUUAAUCGGGGCAGGGUGAGUCGACCCCUAAGGCGAG GCCGAAAGGCGUAGUCGAUGGGAAACAGGUUAAUAUUCCUGUACUUGGUGUUACUGCGAAGGGGGGACGGAGAAGGCUAU GUUGGCCGGGCGACGGUUGUCCCGGUUUAAGCGUGUAGGCUGGUUUUCCAGGCAAAUCCGGAAAAUCAAGGCUGAGGCGU GAUGACGAGGCACUACGGUGCUGAAGCAACAAAUGCCCUGCUUCCAGGAAAAGCCUCUAAGCAUCAGGUAACAUCAAAUC GUACCCCAAACCGACACAGGUGGUCAGGUAGAGAAUACCAAGGCGCUUGAGAGAACUCGGGUGAAGGAACUAGGCAAAAU GGUGCCGUAACUUCGGGAGAAGGCACGCUGAUAUGUAGGUGAGGUCCCUCGCGGAUGGAGCUGAAAUCAGUCGAAGAUAC CAGCUGGCUGCAACUGUUUAUUAAAAACACAGCACUGUGCAAACACGAAAGUGGACGUAUACGGUGUGACGCCUGCCCGG UGCCGGAAGGUUAAUUGAUGGGGUUAGCGCAAGCGAAGCUCUUGAUCGAAGCCCCGGUAAACGGCGGCCGUAACUAUAAC GGUCCUAAGGUAGCGAAAUUCCUUGUCGGGUAAGUUCCGACCUGCACGAAUGGCGUAAUGAUGGCCAGGCUGUCUCCACC CGAGACUCAGUGAAAUUGAACUCGCUGUGAAGAUGCAGUGUACCCGCGGCAAGACGGAAAGACCCCGUGAACCUUUACUA UAGCUUGACACUGAACAUUGAGCCUUGAUGUGUAGGAUAGGUGGGAGGCUUUGAAGUGUGGACGCCAGUCUGCAUGGAGC CGACCUUGAAAUACCACCCUUUAAUGUUUGAUGUUCUAACGUUGACCCGUAAUCCGGGUUGCGGACAGUGUCUGGUGGGU AGUUUGACUGGGGCGGUCUCCUCCUAAAGAGUAACGGAGGAGCACGAAGGUUGGCUAAUCCUGGUCGGACAUCAGGAGGU UAGUGCAAUGGCAUAAGCCAGCUUGACUGCGAGCGUGACGGCGCGAGCAGGUGCGAAAGCAGGUCAUAGUGAUCCGGUGG UUCUGAAUGGAAGGGCCAUCGCUCAACGGAUAAAAGGUACUCCGGGGAUAACAGGCUGAUACCGCCCAAGAGUUCAUAUC GACGGCGGUGUUUGGCACCUCGAUGUCGGCUCAUCACAUCCUGGGGCUGAAGUAGGUCCCAAGGGUAUGGCUGUUCGCCA UUUAAAGUGGUACGCGAGCUGGGUUUAGAACGUCGUGAGACAGUUCGGUCCCUAUCUGCCGUGGGCGCUGGAGAACUGAG GGGGGCUGCUCCUAGUACGAGAGGACCGGAGUGGACGCAUCACUGGUGUUCGGGUUGUCAUGCCAAUGGCACUGCCCGGU AGCUAAAUGCGGAAGAGAUAAGUGCUGAAAGCAUCUAAGCACGAAACUUGCCCCGAGAUGAGUUCUCCCUGACCCUUUAA GGGUCCUGAAGGAACGUUGAAGACGACGACGUUGAUAGGCCGGGUGUGUAAGCGCAGCGAUGCGUUGAGCUAACCGGUAC UAAUGAACCGUGAGGCUUAACCU RNA OTHER nucleic-acid 5S ribosomal RNA 0.038813133 Escherichia coli MRE600 false UGCCUGGCGGCCGUAGCGCGGUGGUCCCACCUGACCCCAUGCCGAACUCAGAAGUGAAACGCCGUAGCGCCGAUGGUAGU GUGGGGUCUCCCCAUGCGAGAGUAGGGAACUGCCAGGCAA RNA OTHER nucleic-acid tRNAfMet 0.024802785 Escherichia coli MRE600 false CGCGGGGUGGAGCAGCCUGGUAGCUCGUCGGGCUCAUAACCCGAAGGUCGUCGGUUCAAAUCCGGCCCCCGCAACCA RNA OTHER nucleic-acid mRNA 0.005844563 Escherichia coli false AGGAGGUAAAAAUGUUCA RNA OTHER nucleic-acid tRNAPhe 0.024485539 Escherichia coli MRE600 false GCCCGGAUAGCUCAGUCGGUAGAGCAGGGGAUUGAAAAUCCCCGUGUCCUUGGUUCGAUUCCGAGUCCGGGCACCA RNA OTHER protein Elongation factor Tu 0.044125215 Escherichia coli 1 false Escherichia coli ligand N-FORMYLMETHIONINE 0.000177221 1 false ligand PHENYLALANINE 0.000165189 1 false ligand GUANOSINE-5'-DIPHOSPHATE 0.000443201 1 false ETHANE
FIELD EMISSION GUN 35.0 BRIGHT FIELD FLOOD BEAM GATAN K2 SUMMIT (4k x 4k) FEI TITAN KRIOS OTHER 300 3218 flexible Correlation coefficient REAL particle 7.5 singleParticle FREALIGN 3.6 FSC 0.143 CUT-OFF C1 7840