REL RCSB RCSB RCSB 2013-11-10 2013-12-25 2014-01-08 2016-02-17 Cryo-electron microscopy of an immature 50S ribosomal subunit (45S particle) from Bacillus subtilis depleted of RbgA (YlqF), conformation 3 Jomaa A Jain N Davis JH Williamson JR Britton RA Ortega J Ribosome assembly, 50S subunit, RbgA, YlqF, GTPase, 45S subunit Jomaa A Jain N Davis JH Williamson JR Britton RA Ortega J Functional domains of the 50S subunit mature late in the assembly process. NUCLEIC ACIDS RES. 42 3419 3435 2014 24335279 doi:10.1093/nar/gkt1295 EMD-5787 FULLOVERLAP EMD-5788 FULLOVERLAP EMD-5789 FULLOVERLAP EMD-5790 FULLOVERLAP EMD-5792 FULLOVERLAP Immature 50S ribosomal subunit (45S particle) from Bacillus subtilis depleted of RbgA (YlqF), conformation 3 Immature 50S ribosomal subunit (45S particle) from Bacillus subtilis depleted of RbgA (YlqF), conformation 3 1 1.6 45S particle false Bacillus subtilis RB301 cytoplasm 1.6 ribosome-prokaryote: LSU 50S, LSU RNA 23S, LSU RNA 5S singleParticle particle 7.5
10 mM Tris-HCl, pH 7.5, 10 mM magnesium acetate, 60 mM ammonium chloride, 3 mM 2-mercaptoethanol
400 mesh holey carbon grids with an additional layer (5-10 nm) of thin carbon. Grids were glow discharged at 5 mA for 15 seconds.
 ETHANE 100 77 FEI VITROBOT MARK III Grids were blotted twice, for 7 seconds each time, before being plunged into liquid ethane. JEOL 2010F FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 1 1.5 3.9 50000.0 GATAN LIQUID NITROGEN 77 77 Objective lens astigmatism was corrected at 300,000 times magnification 2012-02-01 KODAK SO-163 FILM NIKON SUPER COOLSCAN 9000 12.7 300 20 8. Gatan 914 cryo-holder 0 0
ctFFIND
 OTHER 13.0 OTHER Xmipp 21420 emd_5791.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 128 128 128 0 0 0 128 128 128 325.12 325.12 325.12 90.0 90.0 90.0 X Y Z -5.83919239 11.4972086 0.0 1.0 2.54 2.54 2.54 2.0 AUTHOR 3D reconstruction of an immature 50S ribosomal subunit (45S particle) from Bacillus subtilis depleted of RbgA (YlqF), conformation 3
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emd_5791.tif