2018-06-27 REL 2018-04-22 RCSB RCSB 2018-05-09 2018-06-20 Human nuclear exosome-MTR4 RNA complex - focused reconstruction on MTR4-dsDNA/RNA class Weick E-M, Lima CD Weick EM, Puno MR, Januszyk K, Zinder JC, DiMattia MA, Lima CD Helicase-Dependent RNA Decay Illuminated by a Cryo-EM Structure of a Human Nuclear RNA Exosome-MTR4 Complex. Cell 173 1663 1677.e21 2018 29906447 doi:10.1016/j.cell.2018.05.041 1097-4172 emd_7815.map.gz Image stored as Reals 384 384 384 0. 0. 0. 383. 383. 383. 384 384 384 410.88 410.88 410.88 90.0 90.0 90.0 Z X Y -0.07819106 0.16140847 0.000033385753 0.0015468784 1
1.07 1.07 1.07 0.03 Focused refinement for MTR4-dsDNA/RNA within a human nuclear exosome-MTR4 complex 1 Human nuclear exosome-MTR4 helicase captured after unwinding a DNA/RNA substrate
Human C1D/Rrp47 also in the sample, but was not observed in density
protein Human nuclear exosome-MTR4 helicase captured after unwinding a DNA/RNA substrate 0.69
Human C1D/Rrp47 also in the sample, but was not observed in density
Homo sapiens false Escherichia coli ETHANE 298 FEI VITROBOT MARK IV
30 sec wait time, 2.5 sec blot time.
FIELD EMISSION GUN 85.23 BRIGHT FIELD FLOOD BEAM GATAN K2 SUMMIT (4k x 4k) FEI TITAN KRIOS OTHER 300 1439 Correlation coefficient 84. REAL
Models were rebuilt manually using Coot, with real space refinement with local scaling followed by Phenix real space refinement with suboptimal global scaling.
 particle 1.0 8. singleParticle 3.91 FSC 0.143 CUT-OFF
Focused refinement on MTR4-dsDNA/RNA
 C1 33987