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    <admin>
        <lastUpdate>2019-12-25</lastUpdate>
    </admin>
    <deposition>
        <status>REL</status>
        <depositionDate>2016-06-16</depositionDate>
        <depositionSite>RCSB</depositionSite>
        <processingSite>RCSB</processingSite>
        <headerReleaseDate>2016-07-20</headerReleaseDate>
        <mapReleaseDate>2016-10-05</mapReleaseDate>
        <title>Phage Qbeta with icosahedral symmetry</title>
        <authors>Gorzelnik KV, Cui Z, Zhang J</authors>
        <fittedPDBEntryIdList>
            <fittedPDBEntryId>5kip</fittedPDBEntryId>
        </fittedPDBEntryIdList>
        <primaryReference published="true">
            <journalArticle>
                <authors>Gorzelnik KV, Cui Z, Reed CA, Jakana J, Young R, Zhang J</authors>
                <articleTitle>Asymmetric cryo-EM structure of the canonical Allolevivirus Q beta reveals a single maturation protein and the genomic ssRNA in situ.</articleTitle>
                <journal>Proc.Natl.Acad.Sci.USA</journal>
                <volume>113</volume>
                <firstPage>11519</firstPage>
                <lastPage>11524</lastPage>
                <year>2016</year>
                <externalReference type="pubmed">27671640</externalReference>
                <externalReference type="doi">doi:10.1073/pnas.1609482113</externalReference>
                <externalReference type="issn">1091-6490</externalReference>
                <externalReference type="csd">0040</externalReference>
                <externalReference type="astm">PNASA6</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
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        <dataType>Image stored as Reals</dataType>
        <dimensions>
            <numRows>320</numRows>
            <numColumns>320</numColumns>
            <numSections>320</numSections>
        </dimensions>
        <origin>
            <originRow>0.0</originRow>
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            <originSec>0.0</originSec>
        </origin>
        <limit>
            <limitRow>319.0</limitRow>
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            <limitSec>319.0</limitSec>
        </limit>
        <spacing>
            <spacingRow>320</spacingRow>
            <spacingCol>320</spacingCol>
            <spacingSec>320</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">384.0</cellA>
            <cellB units="A">384.0</cellB>
            <cellC units="A">384.0</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
            <minimum>-0.06420996</minimum>
            <maximum>0.0819913</maximum>
            <average>0.00034809398</average>
            <std>0.0046583</std>
        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details />
        <pixelSpacing>
            <pixelX units="A">1.2</pixelX>
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            <pixelZ units="A">1.2</pixelZ>
        </pixelSpacing>
        <contourLevel source="author">0.01</contourLevel>
        <annotationDetails>Icosahedral reconstruction of phage Qbeta</annotationDetails>
    </map>
    <sample>
        <numComponents>2</numComponents>
        <name>bacteriophage Q</name>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>virus</entry>
                <sciName>Enterobacteria phage Qbeta</sciName>
                <virus>
                    <sciSpeciesName ncbiTaxId="39803">Enterobacteria phage Qbeta</sciSpeciesName>
                    <empty>false</empty>
                    <enveloped>false</enveloped>
                    <isolate>SPECIES</isolate>
                    <class>VIRION</class>
                </virus>
            </sampleComponent>
            <sampleComponent componentID="2">
                <entry>protein</entry>
                <sciName>Coat protein</sciName>
                <molWtTheo units="MDa">0.014268071</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="39803">Enterobacteria phage Qbeta</sciSpeciesName>
                    <numCopies>3</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>ETHANE</cryogenName>
            <humidity>100</humidity>
            <temperature units="Kelvin">295</temperature>
            <instrument>FEI VITROBOT MARK III</instrument>
            <details>Plunged into liquid ethane (FEI VITROBOT MARK III). </details>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">50.0</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <detector>GATAN K2 SUMMIT (4k x 4k)</detector>
            <microscope>JEOL 3200FSC</microscope>
            <specimenHolderModel>OTHER</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
            <nominalMagnification>30000.0</nominalMagnification>
        </imaging>
        <imageAcquisition />
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <buffer>
                <ph>7.0</ph>
            </buffer>
        </specimenPreparation>
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        <method>singleParticle</method>
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            <software>RELION</software>
            <resolutionByAuthor>3.7</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
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            <numProjections>51815</numProjections>
        </singleParticle>
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