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<emdEntry accessCode="8638" version="1.9.6" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://github.com/emdb-empiar/emdb-schemas/blob/master/v1/v1_9/emdb_v1_9_6.xsd">
    <admin>
        <lastUpdate>2020-01-29</lastUpdate>
    </admin>
    <deposition>
        <status>REL</status>
        <depositionDate>2017-03-17</depositionDate>
        <depositionSite>RCSB</depositionSite>
        <processingSite>RCSB</processingSite>
        <headerReleaseDate>2017-04-26</headerReleaseDate>
        <mapReleaseDate>2017-08-23</mapReleaseDate>
        <title>Cryo-EM map of the ERAD components Hrd1/Hrd3 dimer</title>
        <authors>Schoebel S, Mi W, Stein A, Rapoport TA, Liao M</authors>
        <primaryReference published="true">
            <journalArticle>
                <authors>Schoebel S, Mi W, Stein A, Ovchinnikov S, Pavlovicz R, DiMaio F, Baker D, Chambers MG, Su H, Li D, Rapoport TA, Liao M</authors>
                <articleTitle>Cryo-EM structure of the protein-conducting ERAD channel Hrd1 in complex with Hrd3.</articleTitle>
                <journal>Nature</journal>
                <volume>548</volume>
                <firstPage>352</firstPage>
                <lastPage>355</lastPage>
                <year>2017</year>
                <externalReference type="pubmed">28682307</externalReference>
                <externalReference type="doi">doi:10.1038/nature23314</externalReference>
                <externalReference type="issn">1476-4687</externalReference>
                <externalReference type="csd">0006</externalReference>
                <externalReference type="astm">NATUAS</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
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        <dataType>Image stored as Reals</dataType>
        <dimensions>
            <numRows>192</numRows>
            <numColumns>192</numColumns>
            <numSections>192</numSections>
        </dimensions>
        <origin>
            <originRow>-96.0</originRow>
            <originCol>-96.0</originCol>
            <originSec>-96.0</originSec>
        </origin>
        <limit>
            <limitRow>95.0</limitRow>
            <limitCol>95.0</limitCol>
            <limitSec>95.0</limitSec>
        </limit>
        <spacing>
            <spacingRow>192</spacingRow>
            <spacingCol>192</spacingCol>
            <spacingSec>192</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">259.2</cellA>
            <cellB units="A">259.2</cellB>
            <cellC units="A">259.2</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
            <minimum>-0.094780415</minimum>
            <maximum>0.15879112</maximum>
            <average>-0.0000624861</average>
            <std>0.008063772</std>
        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details />
        <pixelSpacing>
            <pixelX units="A">1.35</pixelX>
            <pixelY units="A">1.35</pixelY>
            <pixelZ units="A">1.35</pixelZ>
        </pixelSpacing>
        <contourLevel source="author">0.045</contourLevel>
        <annotationDetails>map of Hrd1/Hrd3 dimer filtering to 4.7A and applied with -250 bfactor</annotationDetails>
    </map>
    <sample>
        <numComponents>3</numComponents>
        <name>Hrd1/Hrd3 dimer</name>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>protein</entry>
                <sciName>Hrd1/Hrd3 dimer</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="559292">Saccharomyces cerevisiae S288c</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="4932">Saccharomyces cerevisiae</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="2">
                <entry>protein</entry>
                <sciName>ERAD-associated E3 ubiquitin-protein ligase HRD1</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="559292">Saccharomyces cerevisiae S288c</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="4932">Saccharomyces cerevisiae</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="3">
                <entry>protein</entry>
                <sciName>ERAD-associated E3 ubiquitin-protein ligase component HRD3</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="559292">Saccharomyces cerevisiae S288c</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="4932">Saccharomyces cerevisiae</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>ETHANE</cryogenName>
            <details />
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">82.0</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <detector>GATAN K2 SUMMIT (4k x 4k)</detector>
            <microscope>FEI TITAN KRIOS</microscope>
            <specimenHolderModel>OTHER</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
        </imaging>
        <imageAcquisition />
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <buffer>
                <ph>7.5</ph>
            </buffer>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>singleParticle</method>
        <reconstruction>
            <software>RELION</software>
            <resolutionByAuthor>4.7</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
        </reconstruction>
        <singleParticle>
            <appliedSymmetry>C1</appliedSymmetry>
            <numProjections>139754</numProjections>
        </singleParticle>
    </processing>
</emdEntry>