REL PDBe PDBe PDBe 2005-03-24 2005-04-11 2005-04-11 2012-10-24 Visualizing tmRNA entry into a stalled ribosome. Valle M Gillet R Kaur S Henne A Ramakrishnan V Frank J Valle M Gillet R Kaur S Henne A Ramakrishnan V Frank J Visualizing tmRNA entry into a stalled ribosome. SCIENCE 300 127 130 2003 12677067 doi:10.1126/science.1081798 1zc8 FULLOVERLAP 70S-Psite tRNA - mRNA - EF-Tu.GDP - alanylated tmRNA - SmpB - Kirromycin - S1 70S-Psite tRNA - mRNA - EF-Tu.GDP - alanylated tmRNA - SmpB - Kirromycin - S1 5 70S Ribosome
Thermus thermophilus
false Thermus thermophilus HB8 ribosome-prokaryote: ALL tRNA Thermus thermophilus HB8
P site
TRANSFER SINGLE STRANDED false EF-Tu Thermus thermophilus HB8 false tmRNA Thermus thermophilus HB8 false SmpB Thermus thermophilus HB8 false singleParticle particle 7.5
25 mM Hepes-KOH pH 7.5, 30 mM NH4Cl, 7mM MgCl2, 2 mM ATP, 6 mM PEP, 10g/ml PK
 ETHANE 90 93 HOMEMADE PLUNGER
Vitrification instrument: two side blotting plunger
Blot for 2 seconds before plunging FEI TECNAI F20 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2.0 1.45 3.7 49000.0 52000.0 GATAN LIQUID NITROGEN 93 2002-08-01 KODAK SO-163 FILM ZEISS SCAI 14 44 15 1.2 12. Cryo transfer
defocus groups
 C1 OTHER 13.0 OTHER SPIDER 27644
SPIDER definition
 emd_1122.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 140 140 140 -70 -70 -70 140 140 140 394.8 394.8 394.8 90 90 90 X Y Z -147.485000000000014 313.886000000000024 2.87458 28.770199999999999 2.82 2.82 2.82 54.899999999999999 this is a ribosome consisting 70S + Psite tRNA + mRNA + EF-Tu.GDP + alanylated tmRNA + SmpB + Kirromycin + S1
::::EMDATABANK.org::::EMD-1122::::
 RIGID BODY FIT manual
Protocol: Rigid Body. The domains were separately fitted by manual docking using program O
cross correlation coefficient