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    <admin>
        <current_status>
            <date>2023-11-15</date>
            <code>REL</code>
            <processing_site>RCSB</processing_site>
        </current_status>
        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2021-02-13</deposition>
            <header_release>2021-06-30</header_release>
            <map_release>2021-06-30</map_release>
            <update>2023-11-15</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Science Foundation (NSF, United States)</funding_body>
                <code>NSF-DMR-1533958</code>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>Cryo-EM of KFE8 thinner nanotube (class 2, 2-sub-1)</title>
        <authors_list>
            <author>Wang F</author>
            <author>Gnewou OM</author>
        </authors_list>
        <keywords>filament, self-assembly peptide filament, Cryo-EM, peptide fibril, nanotube, PROTEIN FIBRIL</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Wang F</author>
                    <author order="2">Gnewou O</author>
                    <author order="3">Wang S</author>
                    <author order="4">Osinski T</author>
                    <author order="5">Zuo X</author>
                    <author order="6">Egelman EH</author>
                    <author order="7">Conticello VP</author>
                    <title>Deterministic chaos in the self-assembly of beta sheet nanotubes from an amphipathic oligopeptide.</title>
                    <journal_abbreviation>Matter</journal_abbreviation>
                    <volume>4</volume>
                    <first_page>3217</first_page>
                    <last_page>3231</last_page>
                    <year>2021</year>
                    <external_references type="PUBMED">34632372</external_references>
                    <external_references type="DOI">doi:10.1016/j.matt.2021.06.037</external_references>
                    <external_references type="ISSN">2590-2385</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
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                <emdb_id>EMD-23483</emdb_id>
                <relationship>
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                <emdb_id>EMD-23484</emdb_id>
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            <emdb_reference>
                <emdb_id>EMD-23485</emdb_id>
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            <emdb_reference>
                <emdb_id>EMD-23487</emdb_id>
                <relationship>
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                <pdb_id>7lqh</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
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    <sample>
        <name>self-assembly KFE8 nanotubes</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>self-assembly KFE8 nanotubes</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="32630">synthetic construct</organism>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>KFE8 peptide</name>
                <natural_source database="NCBI">
                    <organism ncbi="32630">synthetic construct</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.0011643499999999998</theoretical>
                </molecular_weight>
                <number_of_copies>144</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>(5CR)KFEFKF(GMA)</string>
                </sequence>
            </protein_or_peptide>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>helical</method>
            <aggregation_state>filament</aggregation_state>
            <specimen_preparation_list>
                <helical_preparation preparation_id="1">
                    <buffer>
                        <ph>4.0</ph>
                    </buffer>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                    </vitrification>
                </helical_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <helical_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K3 (6k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">50.0</average_electron_dose_per_image>
                        </image_recording>
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            </microscopy_list>
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                <image_recording_id>1</image_recording_id>
                <final_reconstruction>
                    <applied_symmetry>
                        <helical_parameters>
                            <delta_z units="Å">3.97</delta_z>
                            <delta_phi units="deg">172.1</delta_phi>
                            <axial_symmetry>C2</axial_symmetry>
                        </helical_parameters>
                    </applied_symmetry>
                    <resolution units="Å" res_type="BY AUTHOR">3.4</resolution>
                    <resolution_method>OTHER</resolution_method>
                    <details>Model:Map FSC 0.38 cut off</details>
                    <number_images_used>31760</number_images_used>
                </final_reconstruction>
                <startup_model type_of_model="NONE"/>
                <final_angle_assignment>
                    <type>NOT APPLICABLE</type>
                </final_angle_assignment>
            </helical_processing>
        </structure_determination>
    </structure_determination_list>
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        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>320</col>
            <row>320</row>
            <sec>320</sec>
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            <row>-160</row>
            <sec>-160</sec>
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            <y>320</y>
            <z>320</z>
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        <cell>
            <a units="Å">345.6</a>
            <b units="Å">345.6</b>
            <c units="Å">345.6</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
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            <medium>Y</medium>
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            <maximum>0.92889005</maximum>
            <average>0.0028297608</average>
            <std>0.029431619</std>
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            <x units="Å">1.08</x>
            <y units="Å">1.08</y>
            <z units="Å">1.08</z>
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        <annotation_details>cryo-EM of KFE8 thinner tube (class 2, 2-sub-1)</annotation_details>
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