2017-07-12 REL PDBe PDBe PDBe 2016-12-06 2016-12-14 2016-12-28 2017-07-12 Cryo-EM structure of the spinach chloroplast ribosome reveals the location of plastid-specific ribosomal proteins and extensions Graf M Wilson D Graf M Arenz S Huter P Donhofer A Novacek J Wilson DN Cryo-EM structure of the spinach chloroplast ribosome reveals the location of plastid-specific ribosomal proteins and extensions. Nucleic Acids Res. UK 45 2887 2896 2017 27986857 doi:10.1093/nar/gkw1272 1362-4962 0389 NARHAD EMD-3526 associated EM volume
Cryo-EM structure of the spinach chloroplast ribosome reveals the location of plastid-specific ribosomal proteins and extensions
 30S subunit of the spinach chloroplast ribosome 30S subunit of the spinach chloroplast ribosome 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 Spinacia oleracea singleParticle particle 7.4 50.0 HEPES 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid 100.0 K(OAc) Potassium acetate 25.0 Mg(OAc)2 Magnesium acetate 6.0 Beta-ME 2-Mercaptoethanol
Solutions were made fresh and filtered previous to usage.
 Quantifoil R3/3 COPPER CARBON HOLEY ETHANE FEI VITROBOT MARK IV
FEI TITAN KRIOS SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 2.7 FEI FALCON II (4k x 4k) INTEGRATING 4096 4096 2.6 1 5AFI 5.4 FSC 0.143 CUT-OFF FREALIGN 9.11 37636 PROJECTION MATCHING PROJECTION MATCHING emd_3526.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 368 368 368 0 0 0 368 368 368 390.448 390.448 390.448 90.0 90.0 90.0 X Y Z -0.4898067 0.7607107 0.0014738983 0.027074244 1.061 1.061 1.061 0.1 AUTHOR