REL RCSB RCSB RCSB 2014-01-17 2014-03-19 2014-03-19 2014-03-19 Single-particle reconstruction of conformation XVII of ligand-free sGC Campbell MG Underbakke ES Potter CS Carragher B Marletta MA soluble guanylate cyclase, conformational heterogeneity Campbell MG Underbakke ES Potter CS Carragher B Marletta MA Single-particle EM reveals the higher-order domain architecture of soluble guanylate cyclase. PROC.NAT.ACAD.SCI.USA 111 2960 2965 2014 24516165 doi:10.1073/pnas.1400711111 EMD-5861 FULLOVERLAP EMD-5862 FULLOVERLAP EMD-5863 FULLOVERLAP EMD-5864 FULLOVERLAP EMD-5865 FULLOVERLAP EMD-5866 FULLOVERLAP EMD-5867 FULLOVERLAP EMD-5868 FULLOVERLAP EMD-5869 FULLOVERLAP EMD-5870 FULLOVERLAP EMD-5871 FULLOVERLAP EMD-5872 FULLOVERLAP EMD-5873 FULLOVERLAP EMD-5874 FULLOVERLAP EMD-5875 FULLOVERLAP EMD-5876 FULLOVERLAP EMD-5878 FULLOVERLAP EMD-5879 FULLOVERLAP EMD-5880 FULLOVERLAP EMD-5881 FULLOVERLAP EMD-5882 FULLOVERLAP EMD-5883 FULLOVERLAP EMD-5884 FULLOVERLAP Soluble Guanylate Cyclase, ligand-free Soluble Guanylate Cyclase, ligand-free Heterodimer 1 0.15 0.15 Soluble Guanylate Cyclase Rattus norvegicus Norwegian rat 0.15 0.15 1 Heterodimer true Spodoptera frugiperda Sf9 pFastBac1/sGCALPHA1 and pFastBac1/sGCBETA1 singleParticle particle 7.5
50 mM TEA, 150 mM NaCl, 5 mM DTT
 NEGATIVE
3 microliters of sample were applied to grid. The specimen was stained twice with 2% uranyl formate, then allowed to air-dry.
Glow discharged C-flat grid with 2-micron-diameter holes overlaid by thin 1.5 nm continuous carbon
 NONE OTHER FEI TECNAI F20 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2 1.2 2.2 80000.0 SIDE ENTRY, EUCENTRIC 298 2013-01-26 TVIPS TEMCAM-F416 (4k x 4k) 2204 35 -55 0
See publication
Each micrograph
 OTHER 30.0 OTHER SPIDER 233 1 emd_5877.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 224 224 224 -112 -112 -112 224 224 224 237.43999 237.43999 237.43999 90.0 90.0 90.0 X Y Z -1.0567857 2.9813385 -0.00077826 0.24257027 1.06 1.06 1.06 0.999 AUTHOR Single-particle reconstruction of conformation XVII of ligand-free sGC
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