2018-10-24 REL RCSB RCSB RCSB 2018-03-28 2018-04-18 2018-09-19 2018-10-24 Single-Molecule 3D Image of neurexin 1 alpha by Individual Particle Electron Tomography (No. 103) Liu JF Misra A Reddy S White MA Ren G Rudenko G Liu J Misra A Reddy MVVVS White MA Ren G Rudenko G Structural Plasticity of Neurexin 1 alpha : Implications for its Role as Synaptic Organizer. J. Mol. Biol. UK 430 4325 4343 2018 30193986 doi:10.1016/j.jmb.2018.08.026 1089-8638 0070 JMOBAK EMD-7761 associated EM volume neurexin 1 alpha neurexin 1 alpha 0 Bos taurus 0.137 unidentified baculovirus tomography particle 8.
25 mM Tris pH 8, 100 mM NaCl, 3 mM CaCl2
 NEGATIVE uranyl formate
The grid was stained for 15 s by sequential submersion in two drops of uranyl formate (UF).
 COPPER 200 CARBON CONTINUOUS GLOW DISCHARGE AIR 0.039
The purified neurexin 1 alpha proteins were stored in 25 mM Tris pH 8, 100 mM NaCl in flash-frozen aliquots.
NO SECTIONING ZEISS LIBRA120PLUS FLOOD BEAM BRIGHT FIELD LAB6 120 2.2 80000. In-column Omega Filter GATAN ULTRASCAN 4000 (4k x 4k) 4096 4096 81 1.0 15.0 1
X-ray speckles in images were removed before alignment and 3D reconstruction.
FOURIER SPACE 15.2 FSC 0.5 CUT-OFF
The 3D reconstruction was performed by Individual-Particle Electron Tomography (IPET).
65 TOMOCTF IMOD
Micrographs were aligned together by IMOD. The CTF was corrected by TOMOCTF.
 emd_7761.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 224 224 224 -112 -112 -112 224 224 224 331.52002 331.52002 331.52002 90.0 90.0 90.0 X Y Z -0.29687908 4.0946627 0.015234168 0.14922062 1.4800001 1.4800001 1.4800001 AUTHOR Single-Molecule 3D Image of neurexin 1 alpha by Individual Particle Electron Tomography (No. 103)