2019-11-20 REL RCSB RCSB RCSB 2017-04-28 2017-05-24 2017-06-07 2019-11-20 Howard Hughes Medical Institute (HHMI) United States American Heart Association 17POST32260003 United States CryoEM structure of Xenopus KCNQ1 channel Mackinnon R Sun J Sun J MacKinnon R Cryo-EM Structure of a KCNQ1/CaM Complex Reveals Insights into Congenital Long QT Syndrome. Cell 169 1042 1050.e9 2017 28575668 doi:10.1016/j.cell.2017.05.019 1097-4172 EMD-8712 associated EM volume
CryoEM structure of Xenopus KCNQ1 channel
 5vms FULLOVERLAP Complex of Xenopus KCNQ1 and CaM Complex of Xenopus KCNQ1 and CaM 0 1 2 Xenopus laevis Homo sapiens HEK293 GnTI- cells 0.3 Potassium voltage-gated channel subfamily KQT member 1 Xenopus laevis 0.062663398 1 Homo sapiens LEVO MATDPPRPTINLDPRVSIYSGRRPLLSRTNIQGRVYNFLERPTGWKCFVYHFTVFLIVLICLIFSVLSTIQQYNNLATET LFWMEIVLVVFFGAEYVVRLWSAGCRSKYVGVWGRLRFARKPISVIDLIVVVASVIVLCVGSNGQVFATSAIRGIRFLQI LRMLHVDRQGGTWRLLGSVVFIHRQELITTLYIGFLGLIFSSYFVYLAEKDAIDSSGEYQFGSYADALWWGVVTVTTIGY GDKVPQTWIGKTIASCFSVFAISFFALPAGILGSGFALKVQQKQRQKHFNRQIPAAASLIQTAWRCYAAENPDSATWKIY IRKQSRNHHLMSPSPKPKKSAMVKKKKIRTERDEGSTDKMLNIPHITYDHVADDRKNDGYSVESYENTVRKPFGFLDPST GPFIRTSSFTDDLDMEGDTLLTPITHISELKEHHRAAIKVIRRMQYFVAKKKFQQARKPYDVRDVIEQYSQGHLNLMVRI KELQRRLDQSLGKPSLFLSVSDKVKDKGINTIGSRLNRVEDKVTQMDHKLNLITDMLHHLLTNQQSNS Calmodulin Homo sapiens 0.016852545 1 Homo sapiens LEVO MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDT DSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK CALCIUM ION 4.0078e-05 3 CA singleParticle particle 5 7.2 ETHANE 298 FEI VITROBOT MARK IV
90-100% humidity.
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 DIRECT ELECTRON DE-64 (8k x 8k) SUPER-RESOLUTION 1.78 1
Generated from 2D class average using EMAN
 C4 3.7 FSC 0.143 CUT-OFF RELION 1.3 FREALIGN 9.03
Entry was refined using space group P4 and cell parameters a=b=c=332.8 and alpha=beta=gamma=90.
69415 PROJECTION MATCHING ANGULAR RECONSTITUTION emd_8712.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 332.8 332.8 332.8 90.0 90.0 90.0 X Y Z -0.037199546 0.061860044 -0.00036935208 0.0018479017 1.3 1.3 1.3 0.016 AUTHOR Xenopus KCNQ1 channel, unsharpened map emd_8712_additional.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 332.8 332.8 332.8 90.0 90.0 90.0 X Y Z -0.09672208 0.14101912 -0.00036935208 0.0035465162 1.3 1.3 1.3 AUTHOR Xenopus KCNQ1 channel, sharpened map