REL PDBe PDBe PDBe 2002-09-24 2002-09-26 2003-09-26 2013-12-04 A cryo-electron microscopic study of ribosome-bound termination factor RF2. Rawat UB Zavialov AV Sengupta J Valle M Grassucci RA Linde J Vestergaard B Ehrenberg M Frank J Rawat UB Zavialov AV Sengupta J Valle M Grassucci RA Linde J Vestergaard B Ehrenberg M Frank J A cryo-electron microscopic study of ribosome-bound termination factor RF2. NATURE 421 87 90 2003 12511960 doi:10.1038/nature01224 1mi6 FULLOVERLAP 1mvr FULLOVERLAP E.coli 70s ribosome-RF2(wild type) complex E.coli 70s ribosome-RF2(wild type) complex 5 2.5 E.coli 70S ribosome false Escherichia coli 2.5 ribosome-prokaryote: LSU 50S P-tRNA Escherichia coli true E-tRNA Escherichia coli 1 false MFTI-mRNA Escherichia coli
Zavialov et al., A posttermination ribosomal complex is the guanine nucleotide exchange factor for peptide release factor RF3. Cell. 107,1-20 (2001).
1 true RF2 (GGQ) wild type Escherichia coli cytoplasm 1 true Escherichia coli BL12 (DE3) pET11a singleParticle particle 0.09 7.5
Polymix buffer,containing at final concentration 5mM potassium phosphate, 5mM magnesium acetate, 5mM ammonium chloride, 95 mM potassium chloride, 0.5 mM calcium chloride, 8mM putrescine, 1mM spermidine and 1mM dithioerythritol
 ETHANE 58 36 HOMEMADE PLUNGER
Vitrification instrument: Two side blotting plunger
Blot and Plunge FEI TECNAI F20 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2.0 2.02 4.5 50000.0 49696.0 GATAN LIQUID NITROGEN 93 2001-10-08 KODAK SO-163 FILM ZEISS SCAI 14 41 20 12. Oxford, Cryo-transfer 3500 0 0
Wiener filtration of defocus groups
 C1 OTHER 12.9 FSC 0.5 CUT-OFF SPIDER/WEB
Frank, J. (2000) Three-Dimensional Cryoelectron Microscopy of Ribosomes, Methods of Enzymology (Ch.18) 317, 276-291.
18199 emd_1007.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 130 130 130 -65 -65 -65 130 130 130 366.6 366.6 366.6 90.0 90.0 90.0 X Y Z -120.430366520000007 233.007385249999999 2.60902333 24.360107419999999 2.82 2.82 2.82 52.299999999999997 AUTHOR Ribosome-bound termination factor RF2
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 1M16 1MVR RIGID BODY FIT O
manual fitting using O