2020-09-30 REL PDBe PDBe PDBe 2020-07-10 2020-09-09 2020-09-09 2020-09-30 Medical Research Council (MRC, United Kingdom) MR/M009858/1 United Kingdom Medical Research Council (MRC, United Kingdom) MC_U105663150 United Kingdom bovine ATP synthase monomer state 2 (combined) Spikes TE Montgomery MG Walker JE Spikes TE Montgomery MG Walker JE Structure of the dimeric ATP synthase from bovine mitochondria. Proc.Natl.Acad.Sci.USA US 117 23519 23526 2020 32900941 doi:10.1073/pnas.2013998117 1091-6490 0040 PNASA6 EMD-11001 other EM volume EMD-11039 other EM volume EMD-11040 other EM volume EMD-11149 other EM volume EMD-11195 other EM volume EMD-11196 other EM volume EMD-11227 other EM volume EMD-11228 other EM volume EMD-11229 other EM volume EMD-11230 other EM volume EMD-11342 other EM volume EMD-11368 associated EM volume
bovine ATP synthase monomer state 2 (combined)
 6zqm FULLOVERLAP Bovine ATP synthase Bovine ATP synthase 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18
bovine ATP synthase inhibited by IF1 1-60His
0.007441 monomeric bovine ATP synthase, state 2 1 1 2 3 4 5 7 8 9 10 11 12 13 14 15 16 17 18
monomeric bovine ATP synthase inhibited by IF1 1-60His, state 2
Bos taurus Bovine ATP synthase 1 6 Bos taurus Escherichia coli ATP synthase subunit alpha, mitochondrial Bovine 0.055302191 3 LEVO EKTGTAEVSSILEERILGADTSVDLEETGRVLSIGDGIARVHGLRNVQAEEMVEFSSGLKGMSLNLEPDNVGVVVFGNDK LIKEGDIVKRTGAIVDVPVGEELLGRVVDALGNAIDGKGPIGSKARRRVGLKAPGIIPRISVREPMQTGIKAVDSLVPIG RGQRELIIGDRQTGKTSIAIDTIINQKRFNDGTDEKKKLYCIYVAIGQKRSTVAQLVKRLTDADAMKYTIVVSATASDAA PLQYLAPYSGCSMGEYFRDNGKHALIIYDDLSKQAVAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNDAFGGGS LTALPVIETQAGDVSAYIPTNVISITDGQIFLETELFYKGIRPAINVGLSVSRVGSAAQTRAMKQVAGTMKLELAQYREV AAFAQFGSDLDAATQQLLSRGVRLTELLKQGQYSPMAIEEQVAVIYAGVRGYLDKLEPSKITKFENAFLSHVISQHQALL GKIRTDGKISEESDAKLKEIVTNFLAGFEA ATP synthase subunit beta, mitochondrial Bovine 0.051757836 3 LEVO AAQASPSPKAGATTGRIVAVIGAVVDVQFDEGLPPILNALEVQGRETRLVLEVAQHLGESTVRTIAMDGTEGLVRGQKVL DSGAPIRIPVGPETLGRIMNVIGEPIDERGPIKTKQFAAIHAEAPEFVEMSVEQEILVTGIKVVDLLAPYAKGGKIGLFG GAGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESGVINLKDATSKVALVYGQMNEPPGARARVALTG LTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADD LTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVARGVQKILQDYKSLQDIIAILGMDEL SEEDKLTVSRARKIQRFLSQPFQVAEVFTGHLGKLVPLKETIKGFQQILAGEYDHLPEQAFYMVGPIEEAVAKADKLAEE HS 7.1.2.2 ATP synthase subunit gamma, mitochondrial Bovine 0.03030076 1 LEVO ATLKDITRRLKSIKNIQKITKSMKMVAAAKYARAERELKPARVYGVGSLALYEKADIKTPEDKKKHLIIGVSSDRGLCGA IHSSVAKQMKSEAANLAAAGKEVKIIGVGDKIRSILHRTHSDQFLVTFKEVGRRPPTFGDASVIALELLNSGYEFDEGSI IFNRFRSVISYKTEEKPIFSLDTISSAESMSIYDDIDADVLRNYQEYSLANIIYYSLKESTTSEQSARMTAMDNASKNAS EMIDKLTLTFNRTRQAVITKELIEIISGAAALD ATP synthase subunit delta, mitochondrial Bovine 0.015074813 1 LEVO AEAAAAQAPAAGPGQMSFTFASPTQVFFNSANVRQVDVPTQTGAFGILAAHVPTLQVLRPGLVVVHAEDGTTSKYFVSSG SVTVNADSSVQLLAEEAVTLDMLDLGAAKANLEKAQSELLGAADEATRAEIQIRIEANEALVKALE ATP synthase subunit epsilon, mitochondrial Bovine 0.005662693 1 LEVO VAYWRQAGLSYIRYSQICAKAVRDALKTEFKANAMKTSGSTIKIVKVKKE ATPase inhibitor, mitochondrial Bos taurus 0.007462098
ATP synthase inhibitor protein IF1 residues 1-60 with a 6His tag
1 Escherichia coli LEVO GSESGDNVRSSAGAVRDAGGAFGKREQAEEERYFRARAKEQLAALKKHHENEISHHAKEIHHHHHH ATP synthase F(0) complex subunit C2, mitochondrial Bovine 0.007653034
Residue 43 is trimethyl-lysine. A postranslational modification.
8 LEVO DIDTAAKFIGAGAATVGVAGSGAGIGTVFGSLIIGYARNPSL(M3L)QQLFSYAILGFALSEAMGLFCLMVAFLILFAM ATP synthase subunit O, mitochondrial Bovine 0.020959777 1 LEVO FAKLVRPPVQIYGIEGRYATALYSAASKQNKLEQVEKELLRVGQILKEPKMAASLLNPYVKRSVKVKSLSDMTAKEKFSP LTSNLINLLAENGRLTNTPAVISAFSTMMSVHRGEVPCTVTTASALDEATLTELKTVLKSFLSKGQVLKLEVKIDPSIMG GMIVRIGEKYVDMSAKTKIQKLSRAMREIL ATP synthase protein 8 Bovine 0.007944523 1 LEVO MPQLDTSTWLTMILSMFLTLFIIFQLKVSKHNFYHNPELTPTKMLKQNTPWETKWTKIYLPLLLPL ATP synthase subunit a Bovine 0.024801785 1 LEVO MNENLFTSFITPVILGLPLVTLIVLFPSLLFPTSNRLVSNRFVTLQQWMLQLVSKQMMSIHNSKGQTWTLMLMSLILFIG STNLLGLLPHSFTPTTQLSMNLGMAIPLWAGAVITGFRNKTKASLAHFLPQGTPTPLIPMLVIIETISLFIQPMALAVRL TANITAGHLLIHLIGGATLALMSISTTTALITFTILILLTILEFAVAMIQAYVFTLLVSLYLHDNT ATP synthase subunit d, mitochondrial Bovine 0.018588256 1 LEVO AGRKLALKTIDWVAFGEIIPRNQKAVANSLKSWNETLTSRLATLPEKPPAIDWAYYKANVAKAGLVDDFEKKFNALKVPI PEDKYTAQVDAEEKEDVKSCAEFLTQSKTRIQEYEKELEKMRNIIPFDQMTIEDLNEVFPETKLDKKKYPYWPHRPIETL ATP synthase subunit f, mitochondrial Bovine 0.010184011 1 LEVO ASVVPLKEKKLLEVKLGELPSWILMRDFTPSGIAGAFQRGYYRYYNKYVNVKKGSIAGLSMVLAAYVFLNYCRSYKELKH ERLRKYH ATP synthase subunit g, mitochondrial Bovine 0.011298196 1 LEVO AEFVRNLAEKAPALVNAAVTYSKPRLATFWYYAKVELVPPTPAEIPTAIQSLKKIINSAKTGSFKQLTVKEALLNGLVAT EVWMWFYVGEIIGKRGIIGYDV ATP synthase subunit ATP5MPL, mitochondrial Bovine 0.006846093 1 LEVO MLQSLIKKVWIPMKPYYTQAYQEIWVGTGLMAYIVYKIRSADKRSKALKASSAAPAHGHH ATP synthase F(0) complex subunit B1, mitochondrial Bovine 0.024702709 1 LEVO PVPPLPEHGGKVRFGLIPEEFFQFLYPKTGVTGPYVLGTGLILYLLSKEIYVITPETFSAISTIGFLVYIVKKYGASVGE FADKLNEQKIAQLEEVKQASIKQIQDAIDMEKSQQALVQKRHYLFDVQRNNIAMALEVTYRERLHRVYREVKNRLDYHIS VQNMMRQKEQEHMINWVEKRVVQSISAQQEKETIAKCIADLKLLSKKAQAQPVM ATP synthase-coupling factor 6, mitochondrial Bovine 0.008971079 1 LEVO NKELDPVQKLFVDKIREYRTKRQTSGGPVDAGPEYQQDLDRELFKLKQMYGKADMNTFPNFTFEDPKFEVVEKPQS ATP synthase membrane subunit DAPIT, mitochondrial Bovine 0.006312383 1 LEVO AGPEADAQFHFTGIKKYFNSYTLTGRMNCVLATYGSIALIVLYFKLRSKKTPAVKAT ATP synthase subunit e, mitochondrial Bovine 0.008205492 1 LEVO VPPVQVSPLIKLGRYSALFLGMAYGAKRYNYLKPRAEEERRLAAEEKKKRDEQKRIERELAEAQEDTILK ADENOSINE-5'-TRIPHOSPHATE 0.000507181 3 ATP MAGNESIUM ION 2.4305e-05 5 MG ADENOSINE-5'-DIPHOSPHATE 0.000427201 3 ADP CARDIOLIPIN 0.001464043 3 CDL 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE 0.00072297 2 LHG water 1.8015e-05 17 HOH singleParticle particle 4.5 7.4 ETHANE 100 294 FEI VITROBOT MARK IV
The sample was allowed to penetrate through the holey support and to distribute to both sides of the grid surface for ca. 15 sec. Then the grids were blotted with filter paper for 8-10 sec before blotting..
Nickel affinity purified filled by gel filtration
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 QUANTUM (4k x 4k) COUNTING 12.0 4.6 1 RELION 3.1 CTFFIND 4.1 2V7Q 3.29 FSC 0.143 CUT-OFF RELION 3.1 90850 MAXIMUM LIKELIHOOD MAXIMUM LIKELIHOOD RELION 3.1 RELION 3.1 emd_11368.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 500 500 500 0 0 0 500 500 500 524.0 524.0 524.0 90.0 90.0 90.0 Z Y X -26.711363 52.28754 0.015261618 1.0967349 1.048 1.048 1.048 8.4 AUTHOR State 2 Composite map generated by PHENIX combinefocussedmaps 2V7Q A 2V7Q B 2V7Q C 2V7Q D 2V7Q E 2V7Q F 2V7Q G 2V7Q H 2V7Q I 2V7Q J 2CLY A 2CLY B 2CLY C RIGID BODY FIT REAL