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    <admin>
        <current_status>
            <date>2023-12-27</date>
            <code>REL</code>
            <processing_site>RCSB</processing_site>
        </current_status>
        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2023-05-17</deposition>
            <header_release>2023-12-27</header_release>
            <map_release>2023-12-27</map_release>
            <update>2023-12-27</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>Open Philanthropy</funding_body>
                <code>68-0683</code>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>Negative stain EM map 4 of polyclonal serum from rabbit immunized with Trihead-Hyperglycosylated-Mosaic-I53_dn5 in complex with MI15 HA-foldon</title>
        <authors_list>
            <author>Dosey A</author>
            <author>King NP</author>
        </authors_list>
        <keywords>Fab, Serum, Influenza Hemagglutinin, Immune Complex, IMMUNE SYSTEM</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Dosey A</author>
                    <author order="2">Ellis D</author>
                    <author order="3">Boyoglu-Barnum S</author>
                    <author order="4">Syeda H</author>
                    <author order="5">Saunders M</author>
                    <author order="6">Watson MJ</author>
                    <author order="7">Kraft JC</author>
                    <author order="8">Pham MN</author>
                    <author order="9">Guttman M</author>
                    <author order="10">Lee KK</author>
                    <author order="11">Kanekiyo M</author>
                    <author order="12">King NP</author>
                    <title>Combinatorial immune refocusing within the influenza hemagglutinin RBD improves cross-neutralizing antibody responses.</title>
                    <journal_abbreviation>Cell Rep</journal_abbreviation>
                    <country>US</country>
                    <volume>42</volume>
                    <first_page>113553</first_page>
                    <last_page>113553</last_page>
                    <year>2023</year>
                    <external_references type="PUBMED">38096052</external_references>
                    <external_references type="DOI">doi:10.1016/j.celrep.2023.113553</external_references>
                    <external_references type="ISSN">2211-1247</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <emdb_list>
            <emdb_reference>
                <emdb_id>EMD-40798</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-40800</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-40801</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-40831</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-40832</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
            </emdb_reference>
        </emdb_list>
    </crossreferences>
    <sample>
        <name>polyclonal serum cleaved to Fabs from rabbit immunized with Trihead-Hyperglycosylated-Mosaic-I53_dn5 in complex with MI15 HA-foldon</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>polyclonal serum cleaved to Fabs from rabbit immunized with Trihead-Hyperglycosylated-Mosaic-I53_dn5 in complex with MI15 HA-foldon</name>
                <parent>0</parent>
            </complex_supramolecule>
            <complex_supramolecule supramolecule_id="2">
                <name>polyclonal serum cleaved to Fabs from rabbit immunized with Trihead-Hyperglycosylated-Mosaic-I53_dn5</name>
                <parent>1</parent>
                <natural_source database="NCBI">
                    <organism ncbi="9986">Oryctolagus cuniculus</organism>
                </natural_source>
            </complex_supramolecule>
            <complex_supramolecule supramolecule_id="3">
                <name>MI15 HA-foldon</name>
                <parent>1</parent>
                <natural_source database="NCBI">
                    <organism ncbi="11320">Influenza A virus</organism>
                    <strain>MI15</strain>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <buffer>
                        <ph>7.5</ph>
                    </buffer>
                    <staining>
                        <type>NEGATIVE</type>
                        <material>Uranyl Acetate</material>
                    </staining>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>TFS TALOS L120C</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>LAB6</electron_source>
                    <acceleration_voltage units="kV">120</acceleration_voltage>
                    <nominal_defocus_min units="µm">1.5</nominal_defocus_min>
                    <nominal_defocus_max units="µm">2.5</nominal_defocus_max>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>FEI CETA (4k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">40.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <startup_model type_of_model="INSILICO MODEL">
                    <details>Ab initio reconstruction</details>
                </startup_model>
                <final_reconstruction>
                    <resolution units="Å" res_type="BY AUTHOR">18.5</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <number_images_used>9876</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>ANGULAR RECONSTITUTION</type>
                    <software_list>
                        <software>
                            <name>cryoSPARC</name>
                        </software>
                    </software_list>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>ANGULAR RECONSTITUTION</type>
                    <software_list>
                        <software>
                            <name>cryoSPARC</name>
                        </software>
                    </software_list>
                </final_angle_assignment>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="28312">
        <file>emd_40802.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>192</col>
            <row>192</row>
            <sec>192</sec>
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            <row>0</row>
            <sec>0</sec>
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        <spacing>
            <x>192</x>
            <y>192</y>
            <z>192</z>
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        <cell>
            <a units="Å">480.0</a>
            <b units="Å">480.0</b>
            <c units="Å">480.0</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
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        <axis_order>
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            <medium>Y</medium>
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            <maximum>5.7693243</maximum>
            <average>-0.023810467</average>
            <std>0.24451216</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">2.5</x>
            <y units="Å">2.5</y>
            <z units="Å">2.5</z>
        </pixel_spacing>
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            <contour primary="true">
                <level>1.75</level>
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-40802::::</label>
        <annotation_details>Negative stain EM map of polyclonal serum from rabbit immunized with Trihead-Hyperglycosylated-Mosaic-I53_dn5 in complex with MI15 HA-foldon.</annotation_details>
    </map>
    <interpretation>
        <half_map_list>
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                <file>emd_40802_half_map_1.map.gz</file>
                <symmetry>
                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>192</col>
                    <row>192</row>
                    <sec>192</sec>
                </dimensions>
                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
                </origin>
                <spacing>
                    <x>192</x>
                    <y>192</y>
                    <z>192</z>
                </spacing>
                <cell>
                    <a units="Å">480.0</a>
                    <b units="Å">480.0</b>
                    <c units="Å">480.0</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-1.2451099</minimum>
                    <maximum>5.983594</maximum>
                    <average>0.003254198</average>
                    <std>0.28961423</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">2.5</x>
                    <y units="Å">2.5</y>
                    <z units="Å">2.5</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-40802::::</label>
                <annotation_details>Negative stain EM map of polyclonal serum from rabbit immunized with Trihead-Hyperglycosylated-Mosaic-I53_dn5 in complex with MI15 HA-foldon.</annotation_details>
            </half_map>
            <half_map format="CCP4" size_kbytes="28312">
                <file>emd_40802_half_map_2.map.gz</file>
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                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>192</col>
                    <row>192</row>
                    <sec>192</sec>
                </dimensions>
                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
                </origin>
                <spacing>
                    <x>192</x>
                    <y>192</y>
                    <z>192</z>
                </spacing>
                <cell>
                    <a units="Å">480.0</a>
                    <b units="Å">480.0</b>
                    <c units="Å">480.0</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-1.0789719</minimum>
                    <maximum>5.9233165</maximum>
                    <average>0.0041057616</average>
                    <std>0.28925395</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">2.5</x>
                    <y units="Å">2.5</y>
                    <z units="Å">2.5</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-40802::::</label>
                <annotation_details>Negative stain EM map of polyclonal serum from rabbit immunized with Trihead-Hyperglycosylated-Mosaic-I53_dn5 in complex with MI15 HA-foldon.</annotation_details>
            </half_map>
        </half_map_list>
    </interpretation>
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