REL RCSB RCSB RCSB 2011-02-16 2011-03-10 2011-03-10 2011-09-06 Seeing the Portal in Herpes Simplex Virus type I B-capsids. Rochat RH Liu X Murata K Nagayama K Rixon FJ Chiu W Herpes, HSV1, Portal, Cryo-EM, Single Particle, UL6, Asymmetric, Symmetry-free Rochat RH Liu X Murata K Nagayama K Rixon FJ Chiu W Seeing the portal in herpes simplex virus type 1 B capsids. J.VIROL. 85 1871 1874 2011 21106752 doi:10.1128/JVI.01663-10 Herpes simplex virus type I B-capsids Herpes simplex virus type I B-capsids Icosahedral 1 100 Herpes Simplex Virus Type I Herpes Simplex Virus Type I Homo sapiens VERTEBRATES 200 vp5 1250 16 vp26 1250 15 vp19 1250 10 vp23 1250 5 OTHER STRAIN false false HSV-1 singleParticle particle
Quantifoil 2/2 grids
 ETHANE 100 85 FEI VITROBOT MARK IV
Vitrification instrument: Vitrobot Mark IV
Blot 1 time for 2 seconds JEOL 2200FS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 4.1 0.0 0.2 40000.0 56000.0 GATAN LIQUID NITROGEN 100 101 100 Astigmatism was corrected at 400,000 times magnification Omega 0.0 20.0 2009-06-10 GENERIC TVIPS (4k x 4k) 20 Side Entry 0 0
The particles were aligned using the multi-path simulated annealing algorithm.
No Correction
 OTHER 24.0 FSC 0.5 CUT-OFF MPSA
Reconstructions were made both with and without imposed symmetry.
6300 emd_5259.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 -150 -150 -150 300 300 300 1596 1596 1596 90 90 90 X Y Z -7.80531 6.50117 0.327607 1.45926 5.32 5.32 5.32 2.4 AUTHOR Icosahedral reconstruction of the HSV-1 B-capsid
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