<emd xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" emdb_id="EMD-1237" version="3.0.1.1" xsi:schemaLocation="https://github.com/emdb-empiar/emdb-schemas/blob/master/v3/v3_0_1_1/emdb_relaxed.xsd">
   <admin>
      <current_status>
         <code>REL</code>
         <processing_site>PDBe</processing_site>
      </current_status>
      <sites>
         <deposition>PDBe</deposition>
         <last_processing>PDBe</last_processing>
      </sites>
      <key_dates>
         <deposition>2006-07-10</deposition>
         <header_release>2006-07-10</header_release>
         <map_release>2007-05-02</map_release>
         <update>2014-04-16</update>
      </key_dates>
      <title>Infectious bursal disease virus capsid assembly and maturation by structural rearrangements of a transient molecular switch.</title>
      <authors_list>
         <author>Luque D</author>
         <author>Saugar I</author>
         <author>Rodriguez JF</author>
         <author>Verdaguer N</author>
         <author>Garriga D</author>
         <author>San Martin C</author>
         <author>Velazquez-Muriel JA</author>
         <author>Trus BL</author>
         <author>Carrascosa JL</author>
         <author>Caston JR</author>
      </authors_list>
      <keywords>
         </keywords>
   </admin>
   <crossreferences>
      <citation_list>
         <primary_citation>
            <journal_citation published="true">
               <author order="1">Luque D</author>
               <author order="2">Saugar I</author>
               <author order="3">Rodriguez JF</author>
               <author order="4">Verdaguer N</author>
               <author order="5">Garriga D</author>
               <author order="6">Martin CS</author>
               <author order="7">Velazquez-Muriel JA</author>
               <author order="8">Trus BL</author>
               <author order="9">Carrascosa JL</author>
               <author order="10">Caston JR</author>
               <title>Infectious bursal disease virus capsid assembly and maturation by structural rearrangements of a transient molecular switch.</title>
               <journal>J.VIROL.</journal>
               <volume>81</volume>
               <first_page>6869</first_page>
               <last_page>6878</last_page>
               <year>2007</year>
               <external_references type="PUBMED">17442720</external_references>
               <external_references type="DOI">doi:10.1128/JVI.00077-07</external_references>
            </journal_citation>
         </primary_citation>
      </citation_list>
   </crossreferences>
   <sample>
      <name>Infectious Bursal Disease Virus Subviral Particle</name>
      <supramolecule_list>
         <sample_supramolecule supramolecule_id="1000">
            <name>Infectious Bursal Disease Virus Subviral Particle</name>
            <oligomeric_state>60 copies of VP2 arranged in 20 trimers</oligomeric_state>
            <number_unique_components>1</number_unique_components>
            <molecular_weight>
               <theoretical units="MDa">2.8</theoretical>
            </molecular_weight>
         </sample_supramolecule>
         <virus_supramolecule supramolecule_id="1">
            <name synonym="Gumboro virus, subviral particle">Infectious bursal disease virus</name>
            <details>IBDV Capsid Protein</details>
            <sci_species_name ncbi="10995">Infectious bursal disease virus</sci_species_name>
            <natural_host database="NCBI">
               <organism ncbi="9031">Gallus gallus</organism>
               <synonym_organism>VERTEBRATES</synonym_organism>
            </natural_host>
            <host_system database="NCBI">
               </host_system>
            <molecular_weight>
               <experimental units="MDa">2.880</experimental>
            </molecular_weight>
            <virus_shell shell_id="1">
               <name>IBDV Subviral particle</name>
               <diameter units="&#8491;">260</diameter>
               <triangulation>1</triangulation>
            </virus_shell>
            <virus_type>VIRUS-LIKE PARTICLE</virus_type>
            <virus_isolate>STRAIN</virus_isolate>
            <virus_enveloped>false</virus_enveloped>
            <virus_empty>true</virus_empty>
            <syn_species_name>Gumboro virus, subviral particle</syn_species_name>
         </virus_supramolecule>
      </supramolecule_list>
   </sample>
   <structure_determination_list>
      <structure_determination structure_determination_id="1">
         <method>singleParticle</method>
         <aggregation_state>particle</aggregation_state>
         <specimen_preparation_list>
            <single_particle_preparation preparation_id="1">
               <buffer>
                  <ph>6.2</ph>
                  <details>25 mM PIPES, 150 mM NaCl, and 20 mM CaCl2</details>
               </buffer>
               <staining>
                  <type>NEGATIVE</type>
                  <details>Samples containing were applied to one side of a holey
        carbon film, washed twice on water drops, blotted, and plunged
        into a liquid ethane bath following standard procedures</details>
               </staining>
               <grid>
                  <details>300 mesh holey carbon film</details>
               </grid>
               <vitrification>
                  <cryogen_name>ETHANE</cryogen_name>
               </vitrification>
            </single_particle_preparation>
         </specimen_preparation_list>
         <microscopy_list>
            <single_particle_microscopy microscopy_id="1">
               <microscope>FEI TECNAI F20</microscope>
               <illumination_mode>FLOOD BEAM</illumination_mode>
               <imaging_mode>BRIGHT FIELD</imaging_mode>
               <electron_source>FIELD EMISSION GUN</electron_source>
               <acceleration_voltage units="kV">200</acceleration_voltage>
               <specimen_holder_model>GATAN LIQUID NITROGEN</specimen_holder_model>
               <image_recording_list>
                  <image_recording>
                     <digitization_details>
                        <scanner>ZEISS SCAI</scanner>
                        <sampling_interval units="&#181;m">7</sampling_interval>
                     </digitization_details>
                     <number_real_images>23</number_real_images>
                     <bits_per_pixel>8.</bits_per_pixel>
                  </image_recording>
               </image_recording_list>
               <specimen_holder>Eucentric</specimen_holder>
            </single_particle_microscopy>
         </microscopy_list>
         <singleparticle_processing image_processing_id="1">
            <ctf_correction>
               <details>Each particle</details>
            </ctf_correction>
            <final_reconstruction>
               <applied_symmetry>
                  <point_group>I</point_group>
               </applied_symmetry>
               <algorithm>OTHER</algorithm>
               <resolution res_type="BY AUTHOR" units="&#8491;">7.2</resolution>
               <resolution_method>FSC 0.33 CUT-OFF</resolution_method>
               <software_list>
                  <software>
                     <name>em3dr2</name>
                  </software>
               </software_list>
               <details>Resolution for SVP was also evaluated by FSC
        calculated       between the full-dataset map and the SVP
        atomic map and for a       correlation limit of 0.3, this was
        at 6.6 A.</details>
               <number_images_used>23754</number_images_used>
            </final_reconstruction>
         </singleparticle_processing>
      </structure_determination>
   </structure_determination_list>
   <map format="CCP4" size_kbytes="56052">
      <file>emd_1237.map.gz</file>
      <symmetry>
         <space_group>1</space_group>
      </symmetry>
      <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
      <dimensions>
         <col>243</col>
         <row>243</row>
         <sec>243</sec>
      </dimensions>
      <origin>
         <col>-121</col>
         <row>-121</row>
         <sec>-121</sec>
      </origin>
      <spacing>
         <x>243</x>
         <y>243</y>
         <z>243</z>
      </spacing>
      <cell>
         <a units="&#8491;">340.2</a>
         <b units="&#8491;">340.2</b>
         <c units="&#8491;">340.2</c>
         <alpha units="deg">90</alpha>
         <beta units="deg">90</beta>
         <gamma units="deg">90</gamma>
      </cell>
      <axis_order>
         <fast>X</fast>
         <medium>Y</medium>
         <slow>Z</slow>
      </axis_order>
      <statistics>
         <minimum>-2.20102</minimum>
         <maximum>3.09582</maximum>
         <average>0.00422405</average>
         <std>0.263223</std>
      </statistics>
      <pixel_spacing>
         <x units="&#8491;">1.4</x>
         <y units="&#8491;">1.4</y>
         <z units="&#8491;">1.4</z>
      </pixel_spacing>
      <contour_list>
         <contour primary="true">
            <level>0.338</level>
         </contour>
      </contour_list>
      <annotation_details>Density map of the T=1 Infectious Bursal
      Disease       Virus (IBDV) Subviral Particle (SVP)2X2Y2Z oriented.</annotation_details>
      <details>::::EMDATABANK.org::::EMD-1237::::</details>
   </map>
   <interpretation>
      <modelling_list>
         <modelling>
            <initial_model>
               <access_code>1WCD</access_code>
            </initial_model>
            <refinement_protocol>RIGID BODY FIT</refinement_protocol>
            <software_list>
               <software>
                  <name>SITUS and URO</name>
               </software>
            </software_list>
            <details>Protocol: Rigid body. Refinement was done in both Real and Fourier Sapce</details>
            <target_criteria>CC and R-factor</target_criteria>
            <refinement_space>REAL</refinement_space>
         </modelling>
         <modelling>
            <initial_model>
               <access_code>2gsy</access_code>
            </initial_model>
            <refinement_protocol>RIGID BODY FIT</refinement_protocol>
            <software_list>
               <software>
                  <name>SITUS and URO</name>
               </software>
            </software_list>
            <details>Protocol: Rigid body. Refinement was done in both Real and Fourier Sapce</details>
            <target_criteria>CC and R-factor</target_criteria>
            <refinement_space>REAL</refinement_space>
         </modelling>
      </modelling_list>
   </interpretation>
</emd>