2023-12-13 REL PDBe PDBe PDBe 2022-09-05 2022-09-21 2022-09-21 2023-12-13 Agence Nationale de la Recherche (ANR) ANR-16-CE11-0017-01 France Agence Nationale de la Recherche (ANR) ANR-18-CE13-0001-01 France Human Frontier Science Program (HFSP) CDA00019/2019-C France Swiss National Science Foundation 310030_192566 Switzerland Microtubule decorated with kinesin-motor domains, 13 protofilaments, 3-start helix, 0 seam, 1 abnormal protofilament Chretien D Guyomar C Cytoplasmic extract, Cytoskeleton, Microtubule, kinesin, STRUCTURAL PROTEIN Guyomar C Bousquet C Ku S Heumann JM Guilloux G Gaillard N Heichette C Duchesne L Steinmetz MO Gibeaux R Chretien D Changes in seam number and location induce holes within microtubules assembled from porcine brain tubulin and in Xenopus egg cytoplasmic extracts. Elife US 11 2022 36503602 doi:10.7554/eLife.83021 2050-084X Guyomar C Bousquet C Ku S Heumann J Guilloux G Gaillard N Heichette C Duchesne L Steinmetz MO Gibeaux R Chretien D Changes in seam number and location induce holes within microtubules assembled from porcine brain tubulin and in Xenopus egg cytoplasmic extracts bioRxiv US 2022 doi:10.1101/2021.07.14.452321 2692-8205 Microtubule assembled in Xenopus egg cytoplasmic extract and decorated with kinesin-motor domain Kif5B Microtubule assembled in Xenopus egg cytoplasmic extract and decorated with kinesin-motor domain Kif5B 0 Xenopus laevis Egg Kinesin-motor domain Kif5B 1 Homo sapiens subtomogramAveraging helicalArray 6.8 80.0 Pipes 1,4-Piperazinediethanesulfonic acid 1.0 MgCl2 Magnesium chloride 1.0 EGTA Ethylene glycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid 1.0 GTP Guanosine triphosphate 0.1 ATP Adenosine triphosphate
pH adjusted with KOH. Buffer used to dilute kinesin-motor domains in the presence of 60 nM gold nanoparticles.
 Quantifoil R2/2 COPPER 200 CARBON HOLEY ARRAY GLOW DISCHARGE AIR ETHANE 95 308.15 LEICA EM GP
Blot for 4 seconds using Whatman paper number 4 from opposite side before plunging.
Microtubule aster formation was induced by addition of 5 percent DMSO in CSF-arrested egg extract. The extract was diluted 1 to 50 in BRB80 containing kinesin-motor domains (2.5 mg/ml) and gold nanoparticles (60 nM) right before freezing.
 FEI TECNAI 20 FLOOD BEAM BRIGHT FIELD LAB6 200 2.0 4.0 6.0 25000.0 GATAN CT3500TR SINGLE TILT ROTATION LIQUID NITROGEN CRYO TRANSFER HOLDER NITROGEN 88.15 93.15
Tilt series were started at 0 degrees and acquired using a Saxton scheme
TVIPS TEMCAM-F416 (4k x 4k) 2048 2048 1.0 1.0
Camera model TVIPS XF416 Images were acquired in binning 2 Electron dose was not calibrated
 1
Camera model TVIPS XF416
45.2 FSC 0.5 CUT-OFF PEET
The picking method involves a large overlap between sub-tomograms. The value provided by the FSC must be taken with caution (no Gold Standard). No FCS curve provided.
45 1 45 NOT APPLICABLE emd_15744.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 58 58 58 0 0 0 58 58 58 515.62 515.62 515.62 90.0 90.0 90.0 X Y Z 97.405990000000003 196.709990000000005 144.703450000000004 11.451768 8.89 8.89 8.89 166.0 AUTHOR Microtubule decorated with kinesin-motor domains, 13 protofilaments, 3-start helix, 0 seam, one aberrant protofilament emd_15744_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 54 54 54 -1 -1 2 54 54 54 480.06003 480.06003 480.06003 90.0 90.0 90.0 X Y Z 0.28931493 255.300250000000005 120.680629999999994 27.312062999999998 8.89 8.89 8.89 AUTHOR Microtubule decorated with kinesin-motor domains, 13 protofilaments, 3-start helix, 0 seam, one aberrant protofilament, reoriented emd_15744_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 58 58 58 0 0 0 58 58 58 515.62 515.62 515.62 90.0 90.0 90.0 X Y Z -51.220005 59.655000000000001 0.00010681287 12.611402 8.89 8.89 8.89 AUTHOR Microtubule decorated with kinesin-motor domains, 13 protofilaments, 3-start helix, 0 seam, one aberrant protofilament, odd half-map emd_15744_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 58 58 58 0 0 0 58 58 58 515.62 515.62 515.62 90.0 90.0 90.0 X Y Z -52.494990000000001 54.167999999999999 0.00008392361 11.966588 8.89 8.89 8.89 AUTHOR Microtubule decorated with kinesin-motor domains, 13 protofilaments, 3-start helix, 0 seam, one aberrant protofilament, even half-map