REL PDBe PDBe PDBe 2009-11-18 2010-01-26 2010-03-10 2012-12-26 CryoEM Model of the Vesicular Stomatitis Virus Ge P Tsao J Green TJ Luo M Zhou ZH NSRV, VSV, CryoEM, RNA, helix Ge P Tsao J Schein S Green TJ Luo M Zhou ZH Cryo-EM model of the bullet-shaped vesicular stomatitis virus. SCIENCE 327 689 693 2010 20133572 doi:10.1126/science.1181766 VSV (Indiana) VSV (Indiana) Mature virion 1 Vesicular stomatitis virus
Horse is the primary host species of the virus but Human is also possible.
Vesicular stomatitis virus Equus caballus VERTEBRATES VIRION STRAIN true false VSV helical filament 7.4
100mM NaCl, 10nM Tris, 1mM EDTA
 NEGATIVE
Cryo sample
Quantifoil 3.5/1 200 mesh
 ETHANE 50 80 HOMEMADE PLUNGER
Vitrification instrument: manual plunger. Vitrification carried out in open room
manual blot 1 second before plunging FEI POLARA 300 SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 2 0.8 2.5 59000.0 98000.0 SIDE ENTRY, EUCENTRIC 80 Objective lens astigmatism was corrected at 250,000 times magnification 2007-01-05 GENERIC TVIPS 1.532 212 20 16. Eucentric OTHER 10.6 FSC 0.5 CUT-OFF EMAN
Helicity determined and applied by IHRSR
 emd_1663.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 -160 -160 -160 320 320 320 490.24 490.24 490.24 90 90 90 X Y Z -6.59838 5.99716 0.00130372 0.998576 1.532 1.532 1.532 1.0 AUTHOR This is one octant of the volume of the top view of the VSV virion trunk.
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 2GIC RIGID BODY FIT Chimera
Protocol: Rigid Body
Cross-correlation REAL 2W2R RIGID BODY FIT Chimera
Protocol: Rigid Body
Cross-correlation REAL emd_1663_msk_2.map emd_1663_msk_2.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 108 112 120 -196 -65 -31 108 112 120 171.584 165.456 183.84 90.0 90.0 90.0 X Y Z -2.970071 5.98934 0.11676611 0.6069033 1.532 1.532 1.532 This is 2N with 5M in closest neighborhood of a central M-hub domain
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 emd_1663_msk_1.map emd_1663_msk_1.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 164 128 84 -179 -153 -11 164 128 84 196.096 251.24802 128.688 90.0 90.0 90.0 X Y Z -3.455964 7.0695634 0.08473596 0.5966924 1.532 1.532 1.532 This is 5 consequetive N's in the nucleocapsid
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