2024-03-20 REL PDBe PDBe PDBe 2023-06-12 2023-12-06 2023-12-06 2024-03-20 Wellcome Trust United Kingdom 11-mer ring of HMPV N-RNA bound to the C-terminal region of P Whitehead JD Decool H Leyrat C Carrique L Fix J Eleouet JF Galloux M Renner M Nucleoprotein, Virus, Nucleocapsid, RNA-binding, HMPV, Pneumoviridae, Mononegavirales, VIRAL PROTEIN Whitehead JD Decool H Leyrat C Carrique L Fix J Eleouet JF Galloux M Renner M Structure of the N-RNA/P interface indicates mode of L/P recruitment to the nucleocapsid of human metapneumovirus. Nat Commun UK 14 7627 7627 2023 37993464 doi:10.1038/s41467-023-43434-5 2041-1723 8pdq FULLOVERLAP Human metapneumovirus N-RNA bound to C-terminal region of P Human metapneumovirus N-RNA bound to C-terminal region of P 0 1 2 3 Nucleoprotein 1 1 Human metapneumovirus (strain CAN97-83) CAN97-83 Human metapneumovirus P C-terminal region 1 2 Human metapneumovirus (strain CAN97-83) CAN97-83 RNA 1 3 Escherichia coli Nucleoprotein Human metapneumovirus (strain CAN97-83) CAN97-83 0.043576519999999994 1 Escherichia coli LEVO MSLQGIHLSDLSYKHAILKESQYTIKRDVGTTTAVTPSSLQQEITLLCGEILYAKHADYKYAAEIGIQYISTALGSERVQ QILRNSGSEVQVVLTRTYSLGKIKNNKGEDLQMLDIHGVEKSWVEEIDKEARKTMATLLKESSGNIPQNQRPSAPDTPII LLCVGALIFTKLASTIEVGLETTVRRANRVLSDALKRYPRMDIPKIARSFYDLFEQKVYHRSLFIEYGKALGSSSTGSKA ESLFVNIFMQAYGAGQTMLRWGVIARSSNNIMLGHVSVQAELKQVTEVYDLVREMGPESGLLHLRQSPKAGLLSLANCPN FASVVLGNASGLGIIGMYRGRVPNTELFSAAESYAKSLKESNKINFSSLGLTDEEKEAAEHFLNVSDDSQNDYE Q6WBA1 Phosphoprotein uman metapneumovirus (strain CAN97-83) 0.0008950750000000001 1 LEVO DIYQLIM Q8B9Q8 RNA Escherichia coli Rosetta2 0.002091315
E.coli RNA, co-purified with N. Due to mixed sequence, modelled as poly-C
1 CCCCCCC singleParticle particle 0.5 8.0
12.5 mM Tris-HCl, pH 8.0, 125 mM NaCl
 ETHANE-PROPANE FEI VITROBOT MARK IV TFS KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 1.0 2.6 FEI TITAN KRIOS AUTOGRID HOLDER GATAN K2 SUMMIT (4k x 4k) 44.2 1
Please see publication for details
CryoSPARC ab initio
 C11 3.1 FSC 0.143 CUT-OFF cryoSPARC 243119 OTHER cryoSPARC
CryoSPARC
 OTHER cryoSPARC
CryoSPARC
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