REL PDBe PDBe PDBe 2011-06-10 2011-07-06 2011-07-06 2014-11-26 Unique structure of iC3b by 3D-Electron Microscopy Alcorlo M Martinez-Barricarte R Fernandez FJ Rodriguez-Gallego C Round A Vega MC Harris CL Rodriguez-de-Cordoba S Llorca O iC3b, C3b, complement, 3D-electron microscopy, SAXS Alcorlo M Martinez Barricarte R Fernandez FJ Rodriguez-Gallego C Round A Vega MC Harris CL Rodriguez de Cordoba S Llorca O Unique structure of iC3b resolved at 24 A resolution by 3D-Electron Microscopy PROC.NAT.ACAD.SCI.USA 108 13236 13240 2011 21788512 doi:10.1073/pnas.1106746108 Human iC3b Human iC3b Monomer 1 0.181 Complement component iC3b Homo sapiens Human Plasma 0.181 1 Monomer false singleParticle particle 1.1 7.4
150 mM NaCl, 20 mM Tris-HCL
 NEGATIVE
1% w/v uranyl acetate
Carbon-coated 400 mesh copper grid
 NONE OTHER JEOL 1230 FLOOD BEAM BRIGHT FIELD TUNGSTEN HAIRPIN 100 2.9 1.0 1.5 50000.0 JEOL Objective lens astigmatism was corrected at 80,000 times magnification KODAK SO-163 FILM OTHER 10.5 112 10
Images scanned with Minolta Dimage Scan Multi Pro scanner at 2400 dpi and averaged to a final 4.2 Angstroms pixel at the specimen
16. Eucentric 0 47
The particles were selected manually with Boxer (Eman)
C1 OTHER 24.0 FSC 0.5 CUT-OFF Xmipp,EMAN 29730 emd_1908.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 72 72 72 -9 -9 -9 72 72 72 302.4 302.4 302.4 90.0 90.0 90.0 X Y Z -13.079293249999999 13.85720444 0.0 0.97025698 4.2 4.2 4.2 4.59 AUTHOR 3D structure of iC3b, a component of the complement alternative pathway
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 2I07 RIGID BODY FIT Situs
Protocol: Rigid Body
Cross-correlation REAL