REL PDBe PDBe PDBe 2011-11-06 2011-12-16 2011-12-21 2012-02-03 Asymmetric cryo-EM reconstruction of E. coli DegQ 12-mer in complex with lysozyme Malet H Canellas F Sawa J Yan J Thalassinos K Ehrmann M Clausen T Saibil HR Chaperone, Protease Malet H Canellas F Sawa J Yan J Thalassinos K Ehrmann M Clausen T Saibil HR Newly folded substrates inside the molecular cage of the HtrA chaperone DegQ. NAT.STRUCT.MOL.BIOL. 19 152 157 2012 22245966 doi:10.1038/nsmb.2210 4a8a FULLOVERLAP Escherichia coli DegQ Gallus gallus lysozyme Escherichia coli DegQ Gallus gallus lysozyme Six lysozyme monomers bind to one DegQ 12-mer 2 0.625 0.625 Native mass spectrometry Size elution chromatography DegQ Escherichia coli K-12 Escherichia coli Periplasm 0.045 0.045 12 Dodecamer true Escherichia coli pET26b Lysozyme Gallus gallus Chicken Egg white 0.0143 0.0143 6 Monomer false GO:0003796 IPR002152 singleParticle particle 0.2 7.5
20 mM HEPES/NaOH, 150 mM NaCl
C-flat grids (CF-2/2-4C-100 Protochips)
 ETHANE HOMEMADE PLUNGER
Vitrification instrument: Manual plunger
Blot for 2 seconds before plunging FEI TECNAI F20 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2 1.0 3.0 50000.0 50000.0 GATAN LIQUID NITROGEN 90 92 91 objective lens astigmatism was corrected at 150,000 times magnification 0
Low dose mode
KODAK SO-163 FILM ZEISS SCAI 7 100 15 8. Single tilt cryo 0 0
Phase flipping
 C1 OTHER 14.2 FSC 0.5 CUT-OFF IMAGIC-5, SPIDER 13432 emd_1981.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 128 128 128 -64 -64 -64 128 128 128 358.4 358.4 358.4 90 90 90 X Y Z -2.79762 40.962499999999999 -0.00000476495 1.00186 2.8 2.8 2.8 0.2 AUTHOR Asymmetric cryo-EM map of E. coli DegQ 12-mer in complex with lysozyme
::::EMDATABANK.org::::EMD-1981::::
 3STJ A B C FLEXIBLE FIT Chimera, Flex-EM
PDBEntryID_givenInChain. Protocol: Rigid body and flexible fitting. Protease and PDZ1 trimers extracted from 3STJ pdb entry. PDZ2 domain modelled with MODELLER from E. coli DegP pdb entry 3CS0.
Cross-correlation, energy REAL 1DPX A RIGID BODY FIT Flex-EM
PDBEntryID_givenInChain. Protocol: Rigid body
Cross-correlation, energy REAL