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            <date>2025-10-22</date>
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        <sites>
            <deposition>PDBe</deposition>
            <last_processing>PDBe</last_processing>
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        <key_dates>
            <deposition>2024-03-12</deposition>
            <header_release>2025-03-26</header_release>
            <map_release>2025-03-26</map_release>
            <update>2025-10-22</update>
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            <grant_reference>
                <funding_body>Swiss National Science Foundation</funding_body>
                <country>Switzerland</country>
            </grant_reference>
        </grant_support>
        <title>Tomogram of nuclear envlope of LBDKO mouse embryonic fibroblast</title>
        <authors_list>
            <author ORCID="0000-0003-4774-7462">Kronenberg-Tenga R</author>
            <author ORCID="0000-0003-0994-2937">Medalia O</author>
        </authors_list>
        <keywords>Nucleosome, FIB-SEM, cryo-ET, Subtomogram-averaging, DNA, histones, DNA BINDING PROTEIN</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author ORCID="0000-0003-4313-4335" order="1">Wang B</author>
                    <author ORCID="0000-0003-4774-7462" order="2">Kronenberg-Tenga R</author>
                    <author order="3">Rosti V</author>
                    <author ORCID="0000-0002-3091-4386" order="4">Di Patrizio Soldateschi E</author>
                    <author ORCID="0000-0001-8839-2739" order="5">Luo Q</author>
                    <author ORCID="0009-0000-5041-922X" order="6">Iannacchero UM</author>
                    <author ORCID="0000-0001-5629-6187" order="7">Pinet L</author>
                    <author ORCID="0000-0003-0638-9347" order="8">Eibauer M</author>
                    <author ORCID="0000-0001-9645-387X" order="9">Boujemaa-Paterski R</author>
                    <author ORCID="0000-0002-5970-4251" order="10">Schuler B</author>
                    <author ORCID="0000-0003-2649-6334" order="11">Lanzuolo C</author>
                    <author ORCID="0000-0003-0994-2937" order="12">Medalia O</author>
                    <title>The molecular basis of lamin-specific chromatin interactions.</title>
                    <journal_abbreviation>Nat.Struct.Mol.Biol.</journal_abbreviation>
                    <country>US</country>
                    <volume>32</volume>
                    <first_page>1999</first_page>
                    <last_page>2011</last_page>
                    <year>2025</year>
                    <external_references type="PUBMED">40750945</external_references>
                    <external_references type="DOI">doi:10.1038/s41594-025-01622-5</external_references>
                    <external_references type="ISSN">1545-9985</external_references>
                </journal_citation>
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        </citation_list>
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                <emdb_id>EMD-19824</emdb_id>
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                <emdb_id>EMD-19827</emdb_id>
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                </relationship>
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            <emdb_reference>
                <emdb_id>EMD-19828</emdb_id>
                <relationship>
                    <other>other EM volume</other>
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            <db_reference>
                <db_name>EMDB</db_name>
                <accession_id>EMD-19824</accession_id>
                <content_type>other EM volume</content_type>
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            <db_reference>
                <db_name>EMDB</db_name>
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                <db_name>EMDB</db_name>
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    <sample>
        <name>Nuclear envelope of FIB-milled lamellae of mouse embryonic fibroblasts</name>
        <supramolecule_list>
            <cell_supramolecule supramolecule_id="1">
                <name>Nuclear envelope of FIB-milled lamellae of mouse embryonic fibroblasts</name>
                <parent>0</parent>
                <natural_source database="NCBI">
                    <organism ncbi="10090">Mus musculus</organism>
                    <tissue>embryonic fibroblast</tissue>
                </natural_source>
            </cell_supramolecule>
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            <method>tomography</method>
            <aggregation_state>cell</aggregation_state>
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                    <buffer>
                        <ph>7.4</ph>
                        <component>
                            <concentration units="x">1.0</concentration>
                            <name>PBS</name>
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                    </buffer>
                    <grid>
                        <model>Quantifoil R2/1</model>
                        <material>GOLD</material>
                        <mesh>200</mesh>
                        <support_film film_type_id="1">
                            <film_material>CARBON</film_material>
                            <film_topology>HOLEY</film_topology>
                        </support_film>
                        <pretreatment>
                            <type>GLOW DISCHARGE</type>
                            <time units="s">30</time>
                            <atmosphere>OTHER</atmosphere>
                        </pretreatment>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <instrument>HOMEMADE PLUNGER</instrument>
                    </vitrification>
                    <sectioning>
                        <focused_ion_beam>
                            <instrument>OTHER</instrument>
                            <ion>OTHER</ion>
                            <voltage>30</voltage>
                            <current>0.02</current>
                            <duration>360</duration>
                            <temperature units="K">118</temperature>
                            <initial_thickness>5000</initial_thickness>
                            <final_thickness>150</final_thickness>
                            <details>The value given for _em_focused_ion_beam.instrument is Zeiss Auriga 40 CrossBeam. This is not in a list of allowed values {'OTHER', 'DB235'} so OTHER is written into the XML file.</details>
                        </focused_ion_beam>
                    </sectioning>
                </tomography_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <tomography_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <c2_aperture_diameter units="µm">50.0</c2_aperture_diameter>
                    <nominal_defocus_min units="µm">4.0</nominal_defocus_min>
                    <nominal_defocus_max units="µm">4.0</nominal_defocus_max>
                    <nominal_magnification>64000.0</nominal_magnification>
                    <specimen_holder_model>FEI TITAN KRIOS AUTOGRID HOLDER</specimen_holder_model>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <specialist_optics>
                        <energy_filter>
                            <slit_width units="eV">20</slit_width>
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                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K2 SUMMIT (4k x 4k)</film_or_detector_model>
                            <detector_mode>COUNTING</detector_mode>
                            <average_exposure_time units="s">2.2</average_exposure_time>
                            <average_electron_dose_per_image units="e/Å^2">4.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </tomography_microscopy>
            </microscopy_list>
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                <image_recording_id>1</image_recording_id>
                <final_reconstruction>
                    <algorithm>BACK PROJECTION</algorithm>
                    <software_list>
                        <software>
                            <name>IMOD</name>
                        </software>
                    </software_list>
                    <number_images_used>35</number_images_used>
                </final_reconstruction>
                <ctf_correction>
                    <software_list>
                        <software>
                            <name>IMOD</name>
                        </software>
                    </software_list>
                    <type>PHASE FLIPPING AND AMPLITUDE CORRECTION</type>
                </ctf_correction>
            </tomography_processing>
        </structure_determination>
    </structure_determination_list>
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        <data_type>IMAGE STORED AS SIGNED INTEGER (2 BYTES)</data_type>
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            <row>928</row>
            <sec>500</sec>
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            <row>-500</row>
            <sec>31</sec>
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            <y>928</y>
            <z>500</z>
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        <cell>
            <a units="Å">4243.2</a>
            <b units="Å">4101.7603</b>
            <c units="Å">2210.0</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
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        <statistics>
            <minimum>-148.0</minimum>
            <maximum>56.0</maximum>
            <average>3.287436</average>
            <std>6.9836726</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">4.42</x>
            <y units="Å">4.42</y>
            <z units="Å">4.42</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-19829::::</label>
        <annotation_details>Tomogram of nuclear envelope in FIB-milled lamella of LBDKO mouse embryonic fibroblast</annotation_details>
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