REL PDBe PDBe PDBe 2011-12-01 2011-12-16 2012-04-17 2012-10-24 ATP-triggered molecular mechanics of the chaperonin GroEL Clare DK Vasishtan D Stagg S Quispe J Farr GW Topf M Horwich AL Saibil HR Tetradecamer of GroEL with ATP bound in one ring Clare DK Vasishtan D Stagg S Quispe J Farr GW Topf M Horwich AL Saibil HR ATP-triggered conformational changes delineate substrate-binding and -folding mechanics of the GroEL chaperonin. CELL(CAMBRIDGE,MASS.) 149 113 123 2012 22445172 doi:10.1016/j.cell.2012.02.047 4aas FULLOVERLAP GroEL-ATP7 Rs_open GroEL-ATP7 Rs_open Tetradecamer of GroEL with 7 ATP molecules bound 2 0.8 0.8 hsp60 Escherichia coli cytoplasm 0.056 0.056
ATPase mutant, D398A
14 tetradecamer true Escherichia coli GO:0042026 IPR002423 ATP synthetic construct 0.00055 0.00055
ATP is bound to seven subunits of one ring
false singleParticle particle 4 7.4
50 mM Tris-HCl pH 7.4, 50 mM KCl, 10 mM MgCl2 and 200uM ATP
cflat grids r2/2
 ETHANE 100 95 OTHER
Vitrification instrument: Vitrobot
Vitrified within 30 seconds Grids were blotted for 2-3 seconds FEI TECNAI F20 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 120 2 0.7 3.5 148500.0 GATAN LIQUID NITROGEN 95 95 95
The data were collected with Leginon at SCRIPPS
GATAN ULTRASCAN 4000 (4k x 4k) 15 Eucentric
The particles were automatically picked using FindEM
Each particle was phase flipped
 C7 OTHER 8.5 FSC 0.5 CUT-OFF SPIDER, IMAGIC
SIRT was used to reconstruct the final map
5500
theta 80-100, phi 0-51.42
 emd_2000.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 -96 -96 -96 192 192 192 387.84 387.84 387.84 90.0 90.0 90.0 X Y Z -1.717103 2.61815715 0.00811408 0.1052276 2.02 2.02 2.02 0.2 AUTHOR The GroEL-ATP7 Rs_open map is one of seven maps calculated from a heterogenous sample
::::EMDATABANK.org::::EMD-2000::::
 1OEL FLEXIBLE FIT Chimera, Flex-EM, NAMD2.6
Protocol: Flexible fitting
Cross-correlation coefficient REAL