Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2)

2024-10-23 REL RCSB RCSB RCSB 2019-05-27 2019-09-18 2019-09-18 2024-10-23 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM29169 United States Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Acosta-Reyes FJ Neupane R Israeli Acute Paralysis Virus, Internal Ribosome Entry Site, IRES, Small Ribosomal Subunit, 40S, RIBOSOME Acosta-Reyes F Neupane R Frank J Fernandez IS The Israeli acute paralysis virus IRES captures host ribosomes by mimicking a ribosomal state with hybrid tRNAs. Embo J. UK 38 e102226 e102226 2019 31609474 doi:10.15252/embj.2019102226 1460-2075 0897 EMJODG EMD-20248 other EM volume

EMD-20248 contains the densities, masks, and half maps for class 1 of the 40S + IAPV-IRES complex

6p4h FULLOVERLAP Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) Structure of a mammalian small ribosomal subunit in complex with the Israeli Acute Paralysis Virus IRES (Class 2) 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 Oryctolagus cuniculus 18S rRNA Oryctolagus cuniculus 0.602776875 1 UACCUGGUUGAUCCUGCCAGUAGCAUAUGCUUGUCUCAAAGAUUAAGCCAUGCAUGUCUAAGUACGCACGGCCGGUACAG UGAAACUGCGAAUGGCUCAUUAAAUCAGUUAUGGUUCCUUUGGUCGCUCGCUCCUCUCCCACUUGGAUAACUGUGGUAAU UCUAGAGCUAAUACAUGCCGACGGGCGCUGACCCCCUUCGCGGGGGGGAUGCGUGCAUUUAUCAGAUCAAAACCAACCCG GUCAGCCCCUCUCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCUUUGGUGACUCUAGAUAACCUCGGGCCGAUCGCACG CCCCCCGUGGCGGCGACGACCCAUUCGAACGUCUGCCCUAUCAACUUUCGAUGGUAGUCGCCGUGCCUACCAUGGUGACC ACGGGUGACGGGGAAUCAGGGUUCGAUUCCGGAGAGGGAGCCUGAGAAACGGCUACCACAUCCAAGGAAGGCAGCAGGCG CGCAAAUUACCCACUCCCGACCCGGGGAGGUAGUGACGAAAAAUAACAAUACAGGACUCUUUCGAGGCCCUGUAAUUGGA AUGAGUCCACUUUAAAUCCUUUAACGAGGAUCCAUUGGAGGGCAAGUCUGGUGCCAGCAGCCGCGGUAAUUCCAGCUCCA AUAGCGUAUAUUAAAGUUGCUGCAGUUAAAAAGCUCGUAGUUGGAUCUUGGGAGCGGGCGGGCGGUCCGCCGCGAGGCGA GCCACCGCCCGUCCCCGCCCCUUGCCUCUCGGCGCCCCCUCGAUGCUCUUAGCUGAGUGUCCCGCGGGGCCCGAAGCGUU UACUUUGAAAAAAUUAGAGUGUUCAAAGCAGGCCCGAGCCGCCUGGAUACCGCAGCUAGGAAUAAUGGAAUAGGACCGCG GUUCUAUUUUGUUGGUUUUCGGAACUGAGGCCAUGAUUAAGAGGGACGGCCGGGGGCAUUCGUAUUGCGCCGCUAGAGGU GAAAUUCUUGGACCGGCGCAAGACGGACCAGAGCGAAAGCAUUUGCCAAGAAUGUUUUCAUUAAUCAAGAACGAAAGUCG GAGGUUCGAAGACGAUCAGAUACCGUCGUAGUUCCGACCAUAAACGAUGCCGACCGGCGAUGCGGCGGCGUUAUUCCCAU GACCCGCCGGGCAGCUUCCGGGAAACCAAAGUCUUUGGGUUCCGGGGGGAGUAUGGUUGCAAAGCUGAAACUUAAAGGAA UUGACGGAAGGGCACCACCAGGAGUGGAGCCUGCGGCUUAAUUUGACUCAACACGGGAAACCUCACCCGGCCCGGACACG GACAGGAUUGACAGAUUGAUAGCUCUUUCUCGAUUCCGUGGGUGGUGGUGCAUGGCCGUUCUUAGUUGGUGGAGCGAUUU GUCUGGUUAAUUCCGAUAACGAACGAGACUCUGGCAUGCUAACUAGUUACGCGACCCCCGAGCGGUCGGCGUCCCCCAAC UUCUUAGAGGGACAAGUGGCGUUCAGCCACCCGAGAUUGAGCAAUAACAGGUCUGUGAUGCCCUUAGAUGUCCGGGGCUG CACGCGCGCUACACUGACUGGCUCAGCGUGUGCCUACCCUACGCCGGCAGGCGCGGGUAACCCGUUGAACCCCAUUCGUG AUGGGGAUCGGGGAUUGCAAUUAUUCCCCAUGAACGAGGAAUUCCCAGUAAGUGCGGGUCAUAAGCUUGCGUUGAUUAAG UCCCUGCCCUUUGUACACACCGCCCGUCGCUACUACCGAUUGGAUGGUUUAGUGAGGCCCUCGGAUCGGCCCCGCCGGGG UCGGCCCACGGCCCUGGCGGAGCGCUGAGAAGACGGUCGAACUUGACUAUCUAGAGGAAGUAAAAGUCGUAACAAGGUUU CCGUAGGUGAACCUGCGGAAGGAUCAUUA uS2 Oryctolagus cuniculus 0.033003069999999995 1 LEVO MSGALDVLQMKEEDVLKFLAAGTHLGGTNLDFQMEQYIYKRKSDGIYIINLKRTWEKLLLAARAIVAIENPADVSVISSR NTGQRAVLKFAAATGATPIAGRFTPGTFTNQIQAAFREPRLLVVTDPQADHQPLMEASYVNLPTIALCNTDSPLHYVDIA IPCNNKGAHSVGLMWWMLAQEVLRMRGTISREHPWEVMPDLYFYRDPEEIEKEEQAAAEKAVTKEEFQGEWTAPEPEFTA TQPEVADWSEGMQVPSVPIQQFPTEDWSAQPATEDWSAAPTAQATEWMGTTTEWS G1TWL4 eS1 Oryctolagus cuniculus 0.030002061 1 LEVO MAVGKNKRLTKGGKKGAKKKVVDPFSKKDWYDVKAPAMFNIRNIGKTLVTRTQGTKIASDGLKGRVFEVSLADLQNDEVA FRKFKLITEDVQGKNCLTNFHGMDLTRDKMCSMVKKWQTMIEAHVDVKTTDGYLLRLFCVGFTKKRNNQIRKTSYAQHQQ VRQIRKKMMEIMTREVQTNDLKEVVNKLIPDSIGKDIEKACQSIYPLHDVFVRKVKMLKKPKFELGKLMELHGEGSSSGK ATGDETGAKVERADGYEPPVQESV G1SS70 uS5 Oryctolagus cuniculus 0.027485208999999997 1 LEVO MADDAGAAGGPGGPGGPGNKEWLPVTKLGRLVKDMKIKSLEEIYLFSLPIKESEIIDFCLGAALKDEVLKIMPVQKQTRA GQRTRFKAFVAIGDYNGHVGLGLKCSKEVATAIRGAIILAKLSIVPVRRGYWGNKIGKPHTVPCKVTGRCGSVLVRLIPA PRGTGIVSAPVPKKLLLMAGIDDCYTSARGCTATLGNFAKATFDAISKTYSYLTPDLWKETVFTKSPYQEFTNHLMKTHT RVSVQRTQAPAVATT G1SWM1 uS3 Oryctolagus cuniculus 0.031146607 1 LEVO MSARRRRRRAAFRRRAEPFIPISVREPLPFLSAARGGKMAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVT PTRTEIIILATRTQNVLGEKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACYGV LRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVLLRQGVLGIKVKIMLPWDPSGKIGPKK PLPDHVSIVEPKDEILPTTPISEQKGGKPEPPAMPQPVPTA G1TNM3 IAPV-IRES Israeli acute paralysis virus 0.08157207799999999 1 GAGCGGUUUCUGGAAUACUAUAUGUAAGUAUAGUGUUCUGGAGGCAUCAUUCUAUGGUUACCCAUCAUUAGAGGAAAUUU CCAAUAAACUCUGGUGUAAGGCUUAGAGUGAUGGUCGAGGUGCCCUAUUUAGGGUGAGGAGCCUCGGUGGCAGCCCCACC AAAUCCUCUAUUGGAUAGGAACAGCUGUACUGGGCAGUUACAGCAGUCGUAUGGUAACACAUGCGGCGUUCCGAAAUACC AUGCCUGGCGAUG EF219380.1 eS4 Oryctolagus cuniculus 0.02965892 1 LEVO MARGPKKHLKRVAAPKHWMLDKLTSVFAPRPSTGPHKLRECLPLIIFLRNKLKYALTGDEVKKICMQRFIKIDGKVRADI TYPAGFMDVISIDKTGENFRLIYDTKGRFAVHRITPEEAKYKLCKVRKIFVGTKGIPHLVTHDARTIRYPDPLIKMNDTI QIDLETGKITDFIKFDTGNLCMVTGGANLGRIGVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVIGKGNKPWISLPR GKGIRLTIAEERDKRLAAKQSSG G1TK17 uS7 Oryctolagus cuniculus 0.022913453 1 LEVO MTEWETAAPAVAETPDIKLFGKWSTDDVQINDISLQDYIAVKEKYAKYLPHSAGRYAAKRFRKAQCPIVERLTNSMMMHG RNNGKKLMTVRIVKHAFEIIHLLTGENPLQVLVNAIINSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGARE AAFRNIKTIAECLADELINAAKGSSNSYAIKKKDELERVAKSNR G1TFM5 eS6 Oryctolagus cuniculus 0.028751905999999997 1 LEVO MKLNISFPATGCQKLIEVDDERKLRTFYEKRMATEVAADALGEEWKGYVVRISGGNDKQGFPMKQGVLTHGRVRLLLSKG HSCYRPRRTGERKRKSVRGCIVDANLSVLNLVIVKKGEKDIPGLTDTTVPRRLGPKRASRIRKLFNLSKEDDVRQYVVRK PLNKEGKKPRTKAPKIQRLVTPRVLQHKRRRIALKKQRTKKNKEEAAEYAKLLAKRMKEAKEKRQEQIAKRRRLSSLRAS TSKSESSQK G1TM55 eS7 Oryctolagus cuniculus 0.022168914 1 LEVO MFSSSAKIVKPNGEKPDEFESGISQALLELEMNSDLKAQLRELNITAAKEIEVGGGRKAIIIFVPVPQLKSFQKIQVRLV RELEKKFSGKHVVFIAQRRILPKPTRKSRTKNKQKRPRSRTLTAVHDAILEDLVFPSEIVGKRIRVKLDGSRLIKVHLDK AQQNNVEHKVETFSGVYKKLTGKDVNFEFPEFQL G1SVB0 eS8 Oryctolagus cuniculus 0.024134206999999998 1 LEVO MGISRDNWHKRRKTGGKRKPYHKKRKYELGRPAANTKIGPRRIHTVRVRGGNKKYRALRLDVGNFSWGSECCTRKTRIID VVYNASNNELVRTKTLVKNCIVLIDSTPYRQWYESHYALPLGRKKGAKLTPEEEEILNKKRSKKIQKKYDERKKNAKISS LLEEQFQQGKLLACIASRPGQCGRADGYVLEGKELEFYLRKIKARKG G1TJW1 uS4 Oryctolagus cuniculus 0.022641563999999996 1 LEVO MPVARSWVCRKTYVTPRRPFEKSRLDQELKLIGEYGLRNKREVWRVKFTLAKIRKAARELLTLDEKDPRRLFEGNALLRR LVRIGVLDEGKMKLDYILGLKIEDFLERRLQTQVFKLGLAKSIHHARVLIRQRHIRVRKQVVNIPSFIVRLDSQKHIDFS LRSPYGGGRPGRVKRKNAKKGQGGAGAGDDEEED B7NZS8 eS10 Oryctolagus cuniculus 0.017156865 1 LEVO MLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLR DYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATE G1TPV3 uS17 Oryctolagus cuniculus 0.018468826 1 LEVO MADIQTERAYQKQPTIFQNKKRVLLGETGKEKLPRYYKNIGLGFKTPKEAIEGTYIDKKCPFTGNVSIRGRILSGVVTKM KMQRTIVIRRDYLHYIRKYNRFEKRHKNMSVHLSPCFRDVQIGDIVTVGECRPLSKTVRFNVLKVTKAAGTKKQFQKF G1TRM4 eS12 Oryctolagus cuniculus 0.014538986999999998 1 LEVO MAEEGIAAGGVMDVNTALQEVLKTALIHDGLARGIREAAKALDKRQAHLCVLASNCDEPMYVKLVEALCAEHQINLIKVD DNKKLGEWVGLCKIDREGKPRKVVGCSCVVVKDYGKESQAKDVIEEYFKCKK G1SFR8 uS15 Oryctolagus cuniculus 0.017259389 1 LEVO MGRMHAPGKGLSQSALPYRRSVPTWLKLTSDDVKEQIYKLAKKGLTPSQIGVILRDSHGVAQVRFVTGNKILRILKSKGL APDLPEDLYHLIKKAVAVRKHLERNRKDKDAKFRLILIESRIHRLARYYKTKRVLPPNWKYESSTASALVA G1SP51 uS11 Oryctolagus cuniculus 0.016302772 1 LEVO MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQD VAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDSTRRKGGRRGRRL G1U472 uS19 Oryctolagus cuniculus 0.017049182 1 LEVO MAEVEQKKKRTFRKFTYRGVDLDQLLDMSYEQLMQLYSARQRRRLSRGLRRKQHSLLKRLRKAKKEAPPMEKPEVVKTHL RDMIILPEMVGSMVGVYNGKTFNQVEIKPEMIGHYLGEFSITYKPVKHGRPGIGATHSSRFIPLK G1U0Q2 uS9 Oryctolagus cuniculus 0.019213465 1 LEVO SARVAPRFPFPVVAPRGEDRHSSAAAMPSKGPLQSVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEP VLLLGKERFAGVDIRVRVKGGGHVAQIYAIRQSISKALVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGG PGARARYQKSYR G1SGX4 eS17 Oryctolagus cuniculus 0.015552119 1 LEVO MGRVRTKTVKKAARVIIEKYYTRLGNDFHTNKRVCEEIAIIPSKKLRNKIAGYVTHLMKRIQRGPVRGISIKLQEEERER RDNYVPEVSALDQEIIEVDPDTKEMLKLLDFGSLSNLQVTQPTVGMNFKTPRGAV G1TU13 uS13 Oryctolagus cuniculus 0.017759776999999997 1 LEVO MSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELTEDEVERVITIMQNPRQYKIP DWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK G1TPG3 eS19 Oryctolagus cuniculus 0.016235796 1 LEVO MPGVTVKDVNQQEFVRALAAFLKKSGKLKVPEWVDTVKLAKHKELAPYDENWFYTRAASTARHLYLRGGAGVGSMTKIYG GRQRNGVMPSHFSRGSKSVARRVLQALEGLKMVEKDQDWGRKLTPQGQRDLDRIAGQVAAAKKKH G1TN62 uS10 Oryctolagus cuniculus 0.013398763000000001 1 LEVO MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKTLRITTRKTPCGEGSKTWDRF QMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA G1SIZ2 eS21 Oryctolagus cuniculus 0.009043276 1 LEVO MQSNAGEFVDLYVPRKCSASNRIIGAKDHASIPMNVAEVDKVTGRFNGQSKTYAICGAIRRMGESDDSILRLAKSHGIVS QNF G1TM82 uS8 Oryctolagus cuniculus 0.014865554999999999 1 LEVO MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRFD VQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF G1TG89 uS12 Oryctolagus cuniculus 0.015784549000000002 1 LEVO AGKCRGLRTARKLRSHRRDQKWHDKQYKKAHLGTALKANPFGGASHAKGIVLEKVGVEAKQPNSAIRKCVRVQLIKNGKK ITAFVPNDGCLNFIEENDEVLVAGFGRKGHAVGDIPGVRFKVVKVANVSLLALYKGKKERPRS G1SZ47 eS24 Oryctolagus cuniculus 0.015548397999999998 1 LEVO MNDTVTIRTRKFMTNRLLQRKQMVIDVLHPGKATVPKTEIREKLAKMYKTTPDVIFVFGFRTHFGGGKTTGFGMIYDSLD YAKKNEPKHRLARHGLYEKKKTSRKQRKERKNRMKKVRGTAKANVGAGKKEPRG G1T3D8 eS25 Oryctolagus cuniculus 0.013776224 1 LEVO MPPKDDKKKKDAGKSAKKDKDPVNKSGGKAKKKKWSKGKVRDKLNNLVLFDKATYDKLCKEVPNYKLITPAVVSERLKIR GSLARAALQELLSKGLIKLVSKHRAQVIYTRNTKGGDAPAAGEDA G1TDB3 eS26 Oryctolagus cuniculus 0.012961455 1 LEVO MTKKRRNNGRAKKGRGHVQPIRCTNCACCVPKDKAIKKFVIRNIVEAAAVRDISEVSVFDAYVLPKLYVKLHYCVSCAIH SKVVRNRSREARKDRTPPPRFRPAGAAPPPPPKPM G1TFE8 eS27 Oryctolagus cuniculus 0.009480186 1 LEVO MPLAKDLLHPSPEEEKRKHKKKRLVQSPNSYFMDVKCPGCYKITTVFSHAQTVVLCVGCSTVLCQPTGGKARLTEGCSFR RKQH G1TZ76 eS28 Oryctolagus cuniculus 0.007855052 1 LEVO MDTSRVQPIKLARVTKVLGRTGSQGQCTQVRVEFMDDTSRSIIRNVKGPVREGDVLTLLESEREARRLR G1TIB4 eS29 Oryctolagus cuniculus 0.006690821 1 LEVO MGHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIGFIKLD G1U7M4 eS30 Oryctolagus cuniculus 0.014498884 1 LEVO MQLFVRAQELHTLEVTGRETVAQIKAHVASLEGIAPEDQVVLLAGTPLEDEATLGQCGVEALSTLEVAGRMLGGKVHGSL ARVGKVRGQTLKVAKQEKKKKRTGRAKRRMQYNRRFVNVVPTFGKKKGPNANS G1T8A2 eS31 Oryctolagus cuniculus 0.018004041000000002 1 LEVO MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGAKKR KKKSYTTPKKNKHKRKKVKLAVLKYYKVDENGKISRLRRECPSDECGAGVFMASHFDRHYCGKCCLTYCFNKPEDK G1SK22 RACK1 Oryctolagus cuniculus 0.035115652 1 LEVO MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALS GSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNS SNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALC FSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR G1SJB4 eL41 Oryctolagus cuniculus 0.003473451 1 LEVO MRAKWRKKRMRRLKRKRRKMRQRSK A0A087WNH4 singleParticle particle 7.5 20.0 Tris-HCl 100.0 KCL 8.0 MgCl2 2.0 DTT Quantifoil R2/2 COPPER CARBON HOLEY 5.0 PLASMA CLEANING 25 OTHER 9.33257

Plasma cleaning for both holey carbon and holey gold grids was done on a Gatan Solarus with Hydrogen (6.4 sccm gas flow) and Oxygen (27.5 sccm gas flow) and 10 W cleaning power.

ETHANE 100 277.15 FEI VITROBOT MARK IV

Ribosomal complexes for the pre-translocated state were assembled at 240-390 nM concentration and applied to plasma treated holey carbon.

7.5 20.0 Tris-HCl 100.0 KCL 8.0 MgCl2 2.0 DTT Quantifoil R2/2 COPPER CARBON HOLEY 5.0 PLASMA CLEANING 25 OTHER 9.33257

Plasma cleaning for both holey carbon and holey gold grids was done on a Gatan Solarus with Hydrogen (6.4 sccm gas flow) and Oxygen (27.5 sccm gas flow) and 10 W cleaning power.

ETHANE 100 277.15 FEI VITROBOT MARK IV

Same sample and buffer conditions used for the holey carbon grids was used for the holey gold grids.

FEI TECNAI F30 OTHER BRIGHT FIELD FIELD EMISSION GUN 300 2.26 0.8 2.5 31000.0 NITROGEN GATAN K2 SUMMIT (4k x 4k) COUNTING 3710 3838 1-40 11234 8.0 42.09 1 1240275 5IT9 C1 3.2 FSC 0.143 CUT-OFF RELION 96826 MAXIMUM LIKELIHOOD RELION MAXIMUM LIKELIHOOD RELION emd_20249.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 312 312 312 0 0 0 312 312 312 384.696 384.696 384.696 90.0 90.0 90.0 X Y Z -0.112940155 0.2127223 0.00042087282 0.0059494916 1.233 1.233 1.233 0.024 AUTHOR ::::EMDATABANK.org::::EMD-20249:::: emd_20249_msk_1.map emd_20249_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 312 312 312 0 0 0 312 312 312 384.696 384.696 384.696 90.0 90.0 90.0 X Y Z -0.017070644 0.05693125 0.00008970808 0.003066846 1.233 1.233 1.233 AUTHOR ::::EMDATABANK.org::::EMD-20249:::: emd_20249_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 312 312 312 0 0 0 312 312 312 384.696 384.696 384.696 90.0 90.0 90.0 X Y Z -0.036187373 0.08414628 0.000087162196 0.00491843 1.233 1.233 1.233 AUTHOR ::::EMDATABANK.org::::EMD-20249:::: emd_20249_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 312 312 312 0 0 0 312 312 312 384.696 384.696 384.696 90.0 90.0 90.0 X Y Z -0.035302836 0.0825528 0.000088846624 0.004923468 1.233 1.233 1.233 AUTHOR ::::EMDATABANK.org::::EMD-20249::::