2020-03-11 REL RCSB RCSB RCSB 2020-01-06 2020-02-05 2020-03-11 2020-03-11 National Institutes of Health/National Institute of General Medical Sciences R01GM118584 United States National Institutes of Health/National Institute of General Medical Sciences GM103310 United States National Institutes of Health/National Institute of Mental Health R01MH114817 United States Other government NYSTAR United States Other private the Simons Foundation (SF349247) United States National Institutes of Health/National Institute of General Medical Sciences F32GM128303 United States human delta protocadherin 1 full ectodomains on membranes tomogram 2 Brasch J Harrisson OJ Noble AJ Carragher B Potter CS Shapiro LS Harrison OJ Brasch J Katsamba PS Ahlsen G Noble AJ Dan H Sampogna RV Potter CS Carragher B Honig B Shapiro L Family-wide Structural and Biophysical Analysis of Binding Interactions among Non-clustered delta-Protocadherins. Cell Rep US 30 2655 2671.e7 2020 32101743 doi:10.1016/j.celrep.2020.02.003 2211-1247 EMD-21191 other EM volume
Tomogram of non-clustered delta 1 protocadherin 1 on membranes
 EMD-21190 other EM volume
Tomogram of non-clustered delta 1 protocadherin 1 on membranes
 EMD-21192 other EM volume
Tomogram of non-clustered delta 2 protocadherin 10 on membranes
 EMD-21193 other EM volume
Tomogram of non-clustered delta 2 protocadherin 10 on membranes
 EMD-21189 associated EM volume trans dimers of protocadherin 1 extracellular domains formed between membranes of liposomes trans dimers of protocadherin 1 extracellular domains formed between membranes of liposomes 0 1
purified full ectodomains of protocadherin 1 are tethered to Ni-NTA lipid head groups presented on the liposome surface through binding of C-terminal hexa-histidine tags
Homo sapiens human delta protocadherin 1 1 1
full ectodomain of human delta protocadherin 1 with a C-terminal hexa histidine tag
 liposome 1
liposomes composed of DOPC and DOGS-NTA (8:2 ratio) with 100nm diameter
 human protocadherin 1 Homo sapiens Homo sapiens LEVO TRVVYKVPEEQPPNTLIGSLAADYGFPDVGHLYKLEVGAPYLRVDGKTGDIFTTETSIDR EGLRECQNQLPGDPCILEFEVSITDLVQNGSPRLLEGQIEVQDINDNTPNFASPVITLAI PENTNIGSLFPIPLASDRDAGPNGVASYELQAGPEAQELFGLQVAEDQEEKQPQLIVMGN LDRERWDSYDLTIKVQDGGSPPRASSALLRVTVLDTNDNAPKFERPSYEAELSENSPIGH SVIQVKANDSDQGANAEIEYTFHQAPEVVRRLLRLDRNTGLITVQGPVDREDLSTLRFSV LAKDRGTNPKSARAQVVVTVKDMNDNAPTIEIRGIGLVTHQDGMANISEDVAEETAVALV QVSDRDEGENAAVTCVVAGDVPFQLRQASETGSDSKKKYFLQTTTPLDYEKVKDYTIEIV AVDSGNPPLSSTNSLKVQVVDVNDNAPVFTQSVTEVAFPENNKPGEVIAEITASDADSGS NAELVYSLEPEPAAKGLFTISPETGEIQVKTSLDREQRESYELKVVAADRGSPSLQGTAT VLVNVLDCNDNDPKFMLSGYNFSVMENMPALSPVGMVTVIDGDKGENAQVQLSVEQDNGD FVIQNGTGTILSSLSFDREQQSTYTFQLKAVDGGVPPRSAYVGVTINVLDENDNAPYITA PSNTSHKLLTPQTRLGETVSQVAAEDFDSGVNAELIYSIAGGNPYGLFQIGSHSGAITLE KEIERRHHGLHRLVVKVSDRGKPPRYGTALVHLYVNETLANRTLLETLLGHSLDTPLDID IAGDPEYERSKQRGNHHHHHH tomography threeDArray 0.7 7.4 100.0 KCl potassium chloride 25.0 HEPES 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid 3.0 CaCl2 Calcium chloride 5.0 glycerol glycerol Homemade COPPER CARBON LACEY PLASMA CLEANING OTHER ETHANE 65 298.15 GATAN CRYOPLUNGE 3
2.5 second blot before plunging.
aggregated liposomes were deposited onto EM grids.
NO SECTIONING FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.001 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN Cs corrector (CEOS GmbH) GIF Bioquantum 20 GATAN K2 QUANTUM (4k x 4k) COUNTING 1 49 1.2 2.84 1 SIMULTANEOUS ITERATIVE (SIRT) TOMO3D part of Appion-protomo 2.4.1 40 emd_21189.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 1176 1281 340 0 0 -340 1176 1281 340 8635.886 9406.946 2496.7695 90.0 90.0 90.0 X Y Z -7.799888 7.7697315 -0.0000000041 0.99999964 7.34344 7.3434396 7.3434396 AUTHOR human delta protocadherin 1 full ectodomains on membranes tomogram 2