REL PDBe PDBe PDBe 2012-06-25 2012-07-09 2012-09-19 2012-10-10 EM map of an influenza H3 hemagglutatin in complex with C05 Fab Khayat R Lee JH Ekiert DC Wilson IA Ward AB Influenza A, flu, hemagglutanin, HA neutralizing antibody, C5 fab, C05 Ekiert DC Kashyap AK Steel J Rubrum A Bhabha G Khayat R Lee JH Dillon MA O'Neil RE Faynboym AM Horowitz M Horowitz L Ward AB Palese P Webby R Lerner RA Bhatt RR Wilson IA Cross-neutralization of influenza A viruses mediated by a single antibody loop. NATURE 489 526 532 2012 22982990 doi:10.1038/nature11414 Influenza HA3 in complex with C5 Fab Influenza HA3 in complex with C5 Fab One HA trimer binds to 3 fabs 2 Influenza A/Perth/16/2009 hemagglutinin Influenza A virus A/Perth/16/2009 Flu 3 Trimer true unidentified baculovirus pDCE198 C05 antibody fab fragment Homo sapiens Human 3 Monomer true unidentified baculovirus pFastBacDual singleParticle particle 0.1 7.5
20mM HEPES pH 7.5, 50mM NaCl
 NEGATIVE
Nano-W for 30 seconds
400 mesh copper grid
 NONE OTHER FEI TECNAI F20 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 120 0.7 0.9 100000.0 SIDE ENTRY, EUCENTRIC 293 293 Objective lens astigmatism corrected at 100,000x
Images taken from 0 to 55 degrees in 5 degree tilt increments
2012-02-14 GENERIC GATAN (4k x 4k) 0.109 269 16
Images recorded using CCD
16. 0 55
Particles were selected using an automated particle picking program.
Micrograph and each particle
 C3 17.0 FSC 0.5 CUT-OFF Sparx 28766 emd_2139.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 100 100 100 -50 -50 -50 100 100 100 218.0 218.0 218.0 90.0 90.0 90.0 X Y Z -1.44750535 8.025697709999999 0.0 1.0 2.18 2.18 2.18 1.4 AUTHOR Negative stain reconstruction of flu HA3 in complex with C5 fab
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