2021-05-05 REL RCSB RCSB RCSB 2021-01-29 2021-02-17 2021-02-17 2021-05-05 National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID) P01-AI138398-S1 United States Structure of the SARS-CoV-2 spike trimer in complex with mRNA vaccine induced neutralizing antibody C669 Barnes CO Bjorkman PJ Wang Z Schmidt F Weisblum Y Muecksch F Barnes CO Finkin S Schaefer-Babajew D Cipolla M Gaebler C Lieberman JA Oliveira TY Yang Z Abernathy ME Huey-Tubman KE Hurley A Turroja M West KA Gordon K Millard KG Ramos V Da Silva J Xu J Colbert RA Patel R Dizon J Unson-O'Brien C Shimeliovich I Gazumyan A Caskey M Bjorkman PJ Casellas R Hatziioannou T Bieniasz PD Nussenzweig MC mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants. Biorxiv US 2021 33501451 doi:10.1101/2021.01.15.426911 Wang Z Schmidt F Weisblum Y Muecksch F Barnes CO Finkin S Schaefer-Babajew D Cipolla M Gaebler C Lieberman JA Oliveira TY Yang Z Abernathy ME Huey-Tubman KE Hurley A Turroja M West KA Gordon K Millard KG Ramos V Silva JD Xu J Colbert RA Patel R Dizon J Unson-O'Brien C Shimeliovich I Gazumyan A Caskey M Bjorkman PJ Casellas R Hatziioannou T Bieniasz PD Nussenzweig MC mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants. Nature UK 2021 33567448 doi:10.1038/s41586-021-03324-6 1476-4687 0006 NATUAS Wang Z Schmidt F Weisblum Y Muecksch F Barnes CO Finkin S Schaefer-Babajew D Cipolla M Gaebler C Lieberman JA Yang Z Abernathy ME Huey-Tubman KE Hurley A Turroja M West KA Gordon K Millard KG Ramos V Da Silva J Xu J Colbert RA Patel R Dizon J Unson-O'Brien C Shimeliovich I Gazumyan A Caskey M Bjorkman PJ Casellas R Hatziioannou T Bieniasz PD Nussenzweig MC mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants. Biorxiv US 2021 33501451 doi:10.1101/2021.01.15.426911 EMD-23398 associated EM volume SARS-CoV-2 S 6P trimer complexed with monoclonal Fab C601 SARS-CoV-2 S 6P trimer complexed with monoclonal Fab C601 0 1 2 0.6 SARS-CoV-2 spike glycoprotein 6P trimer 1 1 Severe acute respiratory syndrome coronavirus 2 Homo sapiens Expi293F pCAGGS 0.585 C669 Fab 1 2 Homo sapiens 0.05 singleParticle particle 3.0 8.0 0.02 Tris-HCl Tris 0.12 NaCl Sodium Chloride ETHANE 100 295 FEI VITROBOT MARK IV FEI TALOS ARCTICA FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 50.0 2.7 0.7000000000000001 2.0 45000.0 GATAN K3 BIOQUANTUM (6k x 4k) 1 1961 3.5 13.0 1
Gain-normalized and collected in counting mode with40 frames per movie
265364
Ab initio volume generated in cryoSPARC
 C1 4.9 FSC 0.143 CUT-OFF cryoSPARC 2.14.2 54129 MAXIMUM LIKELIHOOD cryoSPARC 2.14.2 MAXIMUM LIKELIHOOD cryoSPARC 2.14.2 cryoSPARC 2.14.2 emd_23398.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 432 432 432 0 0 0 432 432 432 375.40802 375.40802 375.40802 90.0 90.0 90.0 X Y Z -0.1401508 0.3933865 -0.00042419677 0.014351953 0.869 0.869 0.869 0.09 AUTHOR sharpened map