2022-08-31 REL RCSB RCSB RCSB 2021-09-19 2022-07-27 2022-07-27 2022-08-31 Department of Energy (DOE, United States) DE-AC02-05CH11231 United States National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI) R01HL115153 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R01GM104427 United States National Institutes of Health/National Institute of Mental Health (NIH/NIMH) R01MH077303 United States National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK) R01DK042667 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R35GM127018 United States National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R01GM032543 United States Molecular Organization of the Early Stages of Nucleosome Phase Separation Visualized by Cryo-Electron Tomography Zhang M Diaz-Celis C Onoa B Canari-Chumpitaz C Requejo KI Liu J Vien M Nogales E Ren G Bustamante C Zhang M Diaz-Celis C Onoa B Canari-Chumpitaz C Requejo KI Liu J Vien M Nogales E Ren G Bustamante C Molecular organization of the early stages of nucleosome phase separation visualized by cryo-electron tomography. Mol.Cell US 82 3000 3014.e9 2022 35907400 doi:10.1016/j.molcel.2022.06.032 1097-2765 2168 MOCEFL EMD-24924 associated EM volume Small spherical type of tetranucleosome condensates Small spherical type of tetranucleosome condensates 0
Small spherical condensates observed at physiological salt condition (20 mM HEPES-KOH pH 7.5; 150 mM NaCl; 5 mM MgCl2; 1 mM DTT) after 20 min incubation.
Xenopus laevis Escherichia coli tomography particle 7.5 ETHANE 95 NO SECTIONING FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K3 BIOQUANTUM (6k x 4k) 40 2.5 7.0 1 40 emd_24924.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 200 0 0 0 512 512 200 5980.16 5980.16 2336.0 90.0 90.0 90.0 X Y Z -1.0554545 3.3397644 0.0022930063 0.04611743 11.68 11.68 11.68 AUTHOR Small spherical tetranucleosome condensate (2)