2024-11-20 REL RCSB RCSB RCSB 2021-09-28 2022-09-28 2022-09-28 2024-11-20 National Institutes of Health/National Cancer Institute (NIH/NCI) CA231466 United States Cryo-EM 3D map of the human Exostosin-1 and Exostosin-2 heterodimer Li H Li H exostosin1, exostosin2, glycosyltransferase, heparan sulfate, TRANSFERASE Li H Chapla D Amos RA Ramiah A Moremen KW Li H Structural basis for heparan sulfate co-polymerase action by the EXT1-2 complex. Nat.Chem.Biol. US 19 565 574 2023 36593275 doi:10.1038/s41589-022-01220-2 1552-4469 7sch FULLOVERLAP hEXT1/2 hEXT1/2 0 1 2 Homo sapiens Exostosin-1 Homo sapiens 0.083404023 1 Mammalia LEVO GFRASRSHSRREEHSGRNGLHHPSPDHFWPRFPDALRPFVPWDQLENEDSSVHISPRQKRDANSSIYKGKKCRMESCFDF TLCKKNGFKVYVYPQQKGEKIAESYQNILAAIEGSRFYTSDPSQACLFVLSLDTLDRDQLSPQYVHNLRSKVQSLHLWNN GRNHLIFNLYSGTWPDYTEDVGFDIGQAMLAKASISTENFRPNFDVSIPLFSKDHPRTGGERGFLKFNTIPPLRKYMLVF KGKRYLTGIGSDTRNALYHVHNGEDVVLLTTCKHGKDWQKHKDSRCDRDNTEYEKYDYREMLHNATFCLVPRGRRLGSFR FLEALQAACVPVMLSNGWELPFSEVINWNQAAVIGDERLLLQIPSTIRSIHQDKILALRQQTQFLWEAYFSSVEKIVLTT LEIIQDRIFKHISRNSLIWNKHPGGLFVLPQYSSYLGDFPYYYANLGLKPPSKFTAVIHAVTPLVSQSQPVLKLLVAAAK SQYCAQIIVLWNCDKPLPAKHRWPATAVPVVVIEGESKVMSSRFLPYDNIITDAVLSLDEDTVLSTTEVDFAFTVWQSFP ERIVGYPARSHFWDNSKERWGYTSKWTNDYSMVLTGAAIYHKYYHYLYSHYLPASLKNMVDQLANCEDILMNFLVSAVTK LPPIKVTQKKQYKETMMGQTSRASRWADPDHFAQRQSCMNTFASWFGYMPLIHSQMRLDPVLFKDQVSILRKKYRDIERL Q16394 2.4.1.224 Exostosin-2 Homo sapiens 0.077238031 1 Mammalia LEVO GWPHSIESSNDWNVEKRSIRDVPVVRLPADSPIPERGDLSCRMHTCFDVYRCGFNPKNKIKVYIYALKKYVDDFGVSVSN TISREYNELLMAISDSDYYTDDINRACLFVPSIDVLNQNTLRIKETAQAMAQLSRWDRGTNHLLFNMLPGGPPDYNTALD VPRDRALLAGGGFSTWTYRQGYDVSIPVYSPLSAEVDLPEKGPGPRQYFLLSSQVGLHPEYREDLEALQVKHGESVLVLD KCTNLSEGVLSVRKRCHKHQVFDYPQVLQEATFCVVLRGARLGQAVLSDVLQAGCVPVVIADSYILPFSEVLDWKRASVV VPEEKMSDVYSILQSIPQRQIEEMQRQARWFWEAYFQSIKAIALATLQIINDRIYPYAAISYEEWNDPPAVKWGSVSNPL FLPLIPPQSQGFTAIVLTYDRVESLFRVITEVSKVPSLSKLLVVWNNQNKNPPEDSLWPKIRVPLKVVRTAENKLSNRFF PYDEIETEAVLAIDDDIIMLTSDELQFGYEVWREFPDRLVGYPGRLHLWDHEMNKWKYESEWTNEVSMVLTGAAFYHKYF NYLYTYKMPGDIKNWVDAHMNCEDIAMNFLVANVTGKAVIKVTPRKKFKCPECTAIDGLSLDQTHMVERSECINKFASVF GTMPLKVVEHRADPVLYKDDFPEKLKSFPNIGSL Q93063 2.4.1.224 singleParticle particle 0.3 7.0 150.0 NaCl sodium chloride 20.0 HEPES HEPES Quantifoil R2/1 GOLD 300 CARBON HOLEY ETHANE 95 299 FEI VITROBOT MARK IV FEI TITAN KRIOS OTHER BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 105000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN 193.0 193.0 0.05 GATAN K3 (6k x 4k) 5760 4092 1 2980 1.5 66.8 1 5186047 C1 3.1 FSC 0.143 CUT-OFF RELION 3.1 120216 ANGULAR RECONSTITUTION ANGULAR RECONSTITUTION emd_25035.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 211.968 211.968 211.968 90.0 90.0 90.0 X Y Z -0.0017398855 1.9930432 0.0022683574 0.035075855 0.828 0.828 0.828 0.0088 AUTHOR AB INITIO MODEL emd_25035_msk_1.map emd_25035_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 211.968 211.968 211.968 90.0 90.0 90.0 X Y Z -0.043505207 0.06840846 0.00001059328 0.003543399 0.828 0.828 0.828 AUTHOR emd_25035_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 211.968 211.968 211.968 90.0 90.0 90.0 X Y Z -0.044573914 0.07139757 0.00000918012 0.0035489374 0.828 0.828 0.828 AUTHOR