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    <admin>
        <current_status>
            <date>2024-06-05</date>
            <code>REL</code>
            <processing_site>RCSB</processing_site>
        </current_status>
        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2021-10-27</deposition>
            <header_release>2022-03-16</header_release>
            <map_release>2022-03-16</map_release>
            <update>2024-06-05</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</funding_body>
                <code>GM122510</code>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>Cryo-EM structure of the enteropathogenic E. coli O127:H6 flagellar filament</title>
        <authors_list>
            <author>Kreutzberger MAB</author>
            <author>Chatterjee S</author>
        </authors_list>
        <keywords>Bacteria flagellar filament, motility, flagellar polymorphism, STRUCTURAL PROTEIN</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Kreutzberger MAB</author>
                    <author order="2">Sobe RC</author>
                    <author order="3">Sauder AB</author>
                    <author order="4">Chatterjee S</author>
                    <author order="5">Pena A</author>
                    <author order="6">Wang F</author>
                    <author order="7">Giron JA</author>
                    <author order="8">Kiessling V</author>
                    <author order="9">Costa TRD</author>
                    <author order="10">Conticello VP</author>
                    <author order="11">Frankel G</author>
                    <author order="12">Kendall MM</author>
                    <author order="13">Scharf BE</author>
                    <author order="14">Egelman EH</author>
                    <title>Flagellin outer domain dimerization modulates motility in pathogenic and soil bacteria from viscous environments.</title>
                    <journal_abbreviation>Nat Commun</journal_abbreviation>
                    <country>UK</country>
                    <volume>13</volume>
                    <first_page>1422</first_page>
                    <last_page>1422</last_page>
                    <year>2022</year>
                    <external_references type="PUBMED">35301306</external_references>
                    <external_references type="DOI">doi:10.1038/s41467-022-29069-y</external_references>
                    <external_references type="ISSN">2041-1723</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>7sn7</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
    </crossreferences>
    <sample>
        <name>Bacterial flagellar filament</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Bacterial flagellar filament</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="168807">Escherichia coli O127:H6</organism>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Flagellin</name>
                <natural_source database="NCBI">
                    <organism ncbi="168807">Escherichia coli O127:H6</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.05613973</theoretical>
                </molecular_weight>
                <number_of_copies>23</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>QVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGAL
SEINNNLQRIRELTVQASTGTNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDLKK
IDSDTLGLNGFNVNGKGETANTAATLKDMSGFTAAAAPGGTVGVTQYTDKSAVASSVDILNAVAGADGNKVTTSADVGFG
TPAAAVTYTYNKDTNSYSAASDDISSANLAAFLNPQARDTTKATVTIGGKDQDVNIDKSGNLTAADDGAVLYMDATGNLT
KNNAGGDTQATLAKVATATGAKAATIQTDKGTFTSDGTAFDGASMSIDANTFANAVKNDTYTATVGAKTYSVTTGSAAAD
TAYMSNGVLSDTPPTYYAQADGSITTTEDAAAGKLVYKGSDGKLTTDTTSKAESTSDPLAALDDAISQIDKFRSSLGAVQ
NRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG</string>
                    <external_references type="UNIPROTKB">A0A2D0NRN6</external_references>
                </sequence>
            </protein_or_peptide>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>filament</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <buffer>
                        <ph>7.2</ph>
                    </buffer>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>TFS KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K3 (6k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">50.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <startup_model type_of_model="PDB ENTRY"/>
                <final_reconstruction>
                    <resolution units="Å" res_type="BY AUTHOR">4.2</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <number_images_used>269269</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>MAXIMUM LIKELIHOOD</type>
                </final_angle_assignment>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
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        <file>emd_25213.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>384</col>
            <row>384</row>
            <sec>384</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>0</row>
            <sec>0</sec>
        </origin>
        <spacing>
            <x>384</x>
            <y>384</y>
            <z>384</z>
        </spacing>
        <cell>
            <a units="Å">414.72003</a>
            <b units="Å">414.72003</b>
            <c units="Å">414.72003</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-1.0468874</minimum>
            <maximum>1.8074155</maximum>
            <average>0.003780077</average>
            <std>0.070000455</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">1.08</x>
            <y units="Å">1.08</y>
            <z units="Å">1.08</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>0.203</level>
                <source>AUTHOR</source>
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        <label>::::EMDATABANK.org::::EMD-25213::::</label>
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