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            <date>2024-01-17</date>
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            <deposition>RCSB</deposition>
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        <key_dates>
            <deposition>2021-10-27</deposition>
            <header_release>2022-05-18</header_release>
            <map_release>2022-05-18</map_release>
            <update>2024-01-17</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</funding_body>
                <code>GM104556</code>
                <country>United States</country>
            </grant_reference>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)</funding_body>
                <code>GM129245</code>
                <country>United States</country>
            </grant_reference>
            <grant_reference>
                <funding_body>National Science Foundation (NSF, United States)</funding_body>
                <code>DBI 1920374</code>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>Slice of a cryo-electron tomogram of PhiPA3-infected Pseudomonas aeruginosa cell at 70 mpi (Cell 2)</title>
        <authors_list>
            <author ORCID="0000-0001-7150-0350">Khanna K</author>
            <author ORCID="0000-0003-4677-9809">Villa E</author>
        </authors_list>
        <keywords>Bacteriophage, Phage Bouquet, Jumbo phage infection, VIRUS</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author ORCID="0000-0003-2793-5394" order="1">Chaikeeratisak V</author>
                    <author ORCID="0000-0001-7150-0350" order="2">Khanna K</author>
                    <author ORCID="0000-0002-1219-053X" order="3">Nguyen KT</author>
                    <author order="4">Egan ME</author>
                    <author ORCID="0000-0001-7550-7752" order="5">Enustun E</author>
                    <author ORCID="0000-0003-0377-1209" order="6">Armbruster E</author>
                    <author ORCID="0000-0002-7808-1045" order="7">Lee J</author>
                    <author ORCID="0000-0002-7868-3345" order="8">Pogliano K</author>
                    <author ORCID="0000-0003-4677-9809" order="9">Villa E</author>
                    <author ORCID="0000-0001-8989-2655" order="10">Pogliano J</author>
                    <title>Subcellular organization of viral particles during maturation of nucleus-forming jumbo phage.</title>
                    <journal_abbreviation>Sci Adv</journal_abbreviation>
                    <country>US</country>
                    <volume>8</volume>
                    <first_page>eabj9670</first_page>
                    <last_page>eabj9670</last_page>
                    <year>2022</year>
                    <external_references type="PUBMED">35507660</external_references>
                    <external_references type="DOI">doi:10.1126/sciadv.abj9670</external_references>
                    <external_references type="ISSN">2375-2548</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
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                <emdb_id>EMD-25219</emdb_id>
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                <emdb_id>EMD-25217</emdb_id>
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            <emdb_reference>
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    <sample>
        <name>Pseudomonas aeruginosa infected with bacteriophage PhiPA3 at 75 mpi</name>
        <supramolecule_list>
            <cell_supramolecule supramolecule_id="1">
                <name>Pseudomonas aeruginosa infected with bacteriophage PhiPA3 at 75 mpi</name>
                <parent>0</parent>
                <natural_source database="NCBI">
                    <organism ncbi="1279007">Pseudomonas aeruginosa PA1</organism>
                </natural_source>
            </cell_supramolecule>
        </supramolecule_list>
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    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>tomography</method>
            <aggregation_state>cell</aggregation_state>
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                <tomography_preparation preparation_id="1">
                    <buffer>
                        <ph>7.0</ph>
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                    <vitrification>
                        <cryogen_name>ETHANE-PROPANE</cryogen_name>
                    </vitrification>
                    <sectioning>
                        <focused_ion_beam>
                            <instrument>OTHER</instrument>
                            <ion>OTHER</ion>
                            <voltage>30</voltage>
                            <current>0.03</current>
                            <duration>1800</duration>
                            <temperature units="K">93</temperature>
                            <initial_thickness>2000</initial_thickness>
                            <final_thickness>150</final_thickness>
                            <details>The value given for _em_focused_ion_beam.instrument is FEI Scios. This is not in a list of allowed values {'DB235', 'OTHER'} so OTHER is written into the XML file.</details>
                        </focused_ion_beam>
                    </sectioning>
                </tomography_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <tomography_microscopy microscopy_id="1">
                    <microscope>FEI POLARA 300</microscope>
                    <illumination_mode>OTHER</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K2 SUMMIT (4k x 4k)</film_or_detector_model>
                            <average_electron_dose_per_image units="e/Å^2">1.0</average_electron_dose_per_image>
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                <image_recording_id>1</image_recording_id>
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                    <number_images_used>60</number_images_used>
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        <data_type>IMAGE STORED AS SIGNED BYTE</data_type>
        <dimensions>
            <col>1236</col>
            <row>1280</row>
            <sec>298</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>0</row>
            <sec>149</sec>
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            <y>1280</y>
            <z>298</z>
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        <cell>
            <a units="Å">27760.559</a>
            <b units="Å">28748.799</b>
            <c units="Å">6693.0796</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
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        <statistics>
            <minimum>-128.0</minimum>
            <maximum>127.0</maximum>
            <average>30.701232999999998</average>
            <std>6.8192835</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">22.46</x>
            <y units="Å">22.46</y>
            <z units="Å">22.46</z>
        </pixel_spacing>
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            <contour primary="true">
                <source>AUTHOR</source>
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        <label>::::EMDATABANK.org::::EMD-25218::::</label>
        <annotation_details>Cryo-electron tomography of PhiPA3-infected Pseudomonas aeruginosa cells at 70 mpi</annotation_details>
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