REL PDBe PDBe PDBe 2014-02-12 2014-03-12 2014-06-11 2014-06-11 SA11 Rotavirus non-trypsinized Triple Layered Particle grown in absence of leupetin Rodriguez JM Chichon FJ Martin-Forero E Gonzalez-Camacho F Carrascosa JL Caston JR Luque D virus assembly, rotavirus, Proteolysis Rodriguez JM Chichon FJ Martin-Forero E Gonzalez-Camacho F Carrascosa JL Caston JR Luque D New insights into rotavirus entry machinery: Stabilization of rotavirus spike conformation is independent of trypsin cleavage PLOS PATHOG. 10 e1004157 e1004157 2014 24873828 doi:10.1371/journal.ppat.1004157 Non-trypsinized Triple Layered Particle from SA11 strain rotavirus grown in absence of leupeptin Non-trypsinized Triple Layered Particle from SA11 strain rotavirus grown in absence of leupeptin icosahedral 1 Simian rotavirus A/SA11 Simian rotavirus A/SA11 SA11 Simiiformes VERTEBRATES VIRION STRAIN false false singleParticle particle 7.4
Tris 25 mM, Na2HPO3 0.7 mM, NaCl 136.9 mM, KCl 5.1 mM, Glucose 5.6 mM, MgCl2 1 mM, CaCl2 1 mM
 NEGATIVE
Samples were applied to grids, blotted and plunged into liquid ethane
R 2/2 Quantifoil grids
 ETHANE OTHER FEI TECNAI F20 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2.26 0.75 3.5 50000.0 50000.0 GATAN LIQUID NITROGEN 2012-05-01 15 91 10 Eucentric
Phase flipping & amplitude decay
 I OTHER 15.9 OTHER Xmipp 1935 emd_2575.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 458 458 458 0 0 0 458 458 458 989.28 989.28 989.28 90.0 90.0 90.0 X Y Z -5.60418606 5.67105913 0.11967961 0.95333248 2.16 2.16 2.16 1.05 AUTHOR 3DR of non-tripsinized SA11 strain rotavirus TLP without leupeptin
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emd_2575.jpg