2022-11-16 REL RCSB RCSB RCSB 2022-09-13 2022-11-02 2022-11-02 2022-11-16 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM56838 United States PaFS prenyltransferase core with single interacting cyclase domain - North class A Faylo JL van Eeuwen T Christianson DW Faylo JL van Eeuwen T Gupta K Murakami K Christianson DW Transient Prenyltransferase-Cyclase Association in Fusicoccadiene Synthase, an Assembly-Line Terpene Synthase. Biochemistry US 61 2417 2430 2022 36227241 doi:10.1021/acs.biochem.2c00509 0006-2960 0033 BICHAW EMD-27989 other EM volume
High-resolution map of prenyltransferase core
 EMD-28046 other EM volume
symmetry expanded consensus map with single cyclase domain
 EMD-28076 other EM volume
symmetry expanded map with cyclase domain class A
 EMD-28079 other EM volume
symmetry expanded map with cyclase domain class B
 EMD-28131 associated EM volume Octamer of fusicoccadiene synthase with a single interacting cyclase domain, in presence of magnesium cofactor and inhibitor pamidronate Octamer of fusicoccadiene synthase with a single interacting cyclase domain, in presence of magnesium cofactor and inhibitor pamidronate 0 1 Diaporthe amygdali Escherichia coli Fusicoccadiene synthase Diaporthe amygdali Escherichia coli LEVO MGSSHHHHHHSSGLVPRGSHMEFKYSEVVEPSTYYTEGLCEGIDVRKSKFTTLEDRGAIRAHEDWNKHIGPCREYRGTLGPRFSFISVAVPECIPERLEVISYANEFAFLHDDVTDHVGHDTGEVENDEMMTVFLEAAHTGAIDTSNKVDIRRAGKKRIQSQLFLEMLAIDPECAKTTMKSWARFVEVGSSRQHETRFVELAKYIPYRIMDVGEMFWFGLVTFGLGLHIPDHELELCRELMANAWIAVGLQNDIWSWPKERDAATLHGKDHVVNAIWVLMQEHQTDVDGAMQICRKLIVEYVAKYLEVIEATKNDESISLDLRKYLDAMLYSISGNVVWSLECPRYNPDVSFNKTQLEWMRQGLPSLESCPVLARSPEIDSDESAVSPTADESDSTEDSLGSGSRQDSSLSTGLSLSPVHSNEGKDLQRVDTDHIFFEKAVLEAPYDYIASMPSKGVRDQFIDALNDWLRVPDVKVGKIKDAVRVLHNSSLLLDDFQDNSPLRRGKPSTHNIFGSAQTVNTATYSIIKAIGQIMEFSAGESVQEVMNSIMILFQGQAMDLFWTYNGHVPSEEEYYRMIDQKTGQLFSIATSLLLNAADNEIPRTKIQSCLHRLTRLLGRCFQICDDYQNLVSADYTKQKGFCEDLDEGKWSLALIHMIHKQRSHMALLNVLSTGRKHGGMTLEQKQFVLDIIEEEKSLDYTRSVMMDLHVQLRAEIGRIEILLDSPNPAMRLLLELLRV 4.2.3.43 singleParticle particle 7.5 ETHANE FEI TITAN KRIOS SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 1.0 2.5 GATAN K3 (6k x 4k) 52.0 1 7.8 FSC 0.143 CUT-OFF 7755 COMMON LINE MAXIMUM LIKELIHOOD emd_28131.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 0 0 0 192 192 192 318.72 318.72 318.72 90.0 90.0 90.0 X Y Z -0.015057999 0.04222741 0.00017454848 0.0027532312 1.66 1.66 1.66 0.015 AUTHOR PaFS prenyltransferase with density for north cyclase domain class A emd_28131_msk_1.map emd_28131_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 0 0 0 192 192 192 318.72 318.72 318.72 90.0 90.0 90.0 X Y Z -0.039676752 0.0630941 0.00017458093 0.004162571 1.66 1.66 1.66 AUTHOR Half-map 1 from final refinement emd_28131_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 0 0 0 192 192 192 318.72 318.72 318.72 90.0 90.0 90.0 X Y Z -0.038785506 0.062362608 0.00017469657 0.0041857744 1.66 1.66 1.66 AUTHOR Half-map 2 from final refinement