2024-06-05 REL RCSB RCSB RCSB 2022-10-03 2023-10-18 2023-10-18 2024-06-05 National Institutes of Health/National Institute of Biomedical Imaging and Bioengineering (NIH/NIBIB) United States Cryo-ET 3D reconstruction of an individual di-nucleosome particle in 5 mM NaCl and 20 mM HEPES buffer --- Particle #076 Zhang M Celis CD Liu JF Bustamante C Ren G Nucleosome Array, DNA BINDING PROTEIN Zhang M Diaz-Celis C Liu J Tao J Ashby PD Bustamante C Ren G Angle between DNA linker and nucleosome core particle regulates array compaction revealed by individual-particle cryo-electron tomography. Nat Commun UK 15 4395 4395 2024 38782894 doi:10.1038/s41467-024-48305-1 2041-1723 Dinucleosome array Dinucleosome array 0 Xenopus laevis tomography particle 7.5
5 mM NaCl 20 mM HEPES 1 mM DTT 1 mM EDTA
 ETHANE 99 277 NO SECTIONING FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.5 3.5 GATAN K2 QUANTUM (4k x 4k) 2.0 5.4 1 SPIDER 32 emd_28362.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 200 200 200 -100 -100 -100 200 200 200 1480.0 1480.0 1480.0 90.0 90.0 90.0 X Y Z -0.20519474 1.0155108 0.000393422 0.012064612 7.4 7.4 7.4 AUTHOR Cryo-ET 3D reconstruction of an individual di-nucleosome particle in 5 mM NaCl and 20 mM HEPES buffer --- Particle #076 FLEXIBLE FIT