2023-12-20 REL RCSB RCSB RCSB 2022-12-10 2023-11-01 2023-11-01 2023-12-20 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) GM118099 United States Cryo-EM structure of the GR-Hsp90-FKBP52 complex Noddings CM Agard DA chaperone, steroid hormone receptor, ligand binding, ATP binding, protein folding Noddings CM Johnson JL Agard DA Cryo-EM reveals how Hsp90 and FKBP immunophilins co-regulate the glucocorticoid receptor. Nat.Struct.Mol.Biol. US 30 1867 1877 2023 37945740 doi:10.1038/s41594-023-01128-y 1545-9985 EMD-29069 other EM volume 8ffv FULLOVERLAP Complex of the Glucocorticoid Receptor ligand binding domain, Hsp90 alpha dimer, and the co-chaperone FKBP52 Complex of the Glucocorticoid Receptor ligand binding domain, Hsp90 alpha dimer, and the co-chaperone FKBP52 0 1 2 3 Homo sapiens 0.307 Heat shock protein HSP 90-alpha Homo sapiens 0.084650531 2 Escherichia coli BL21(DE3) LEVO PEETQTQDQPMEEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTDPSKLDSGKELHINLI PNKQDRTLTIVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVTVITKHNDDEQYA WESSAGGSFTVRTDTGEPMGRGTKVILHLKEDQTEYLEERRIKEIVKKHSQFIGYPITLFVEKERDKEVSDDEAEEKEDK EEEKEKEEKESEDKPEIEDVGSDEEEEKKDGDKKKKKKIKEKYIDQEELNKTKPIWTRNPDDITNEEYGEFYKSLTNDWE DHLAVKHFSVEGQLEFRALLFVPRRAPFDLFENRKKKNNIKLYVRRVFIMDNCEELIPEYLNFIRGVVDSEDLPLNISRE MLQQSKILKVIRKNLVKKCLELFTELAEDKENYKKFYEQFSKNIKLGIHEDSQNRKKLSELLRYYTSASGDEMVSLKDYC TRMKENQKHIYYITGETKDQVANSAFVERLRKHGLEVIYMIEPIDEYCVQQLKEFEGKTLVSVTKEGLELPEDEEEKKKQ EEKKTKFENLCKIMKDILEKKVEKVVVSNRLVTSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMAAKKHLEINPDHS IIETLRQKAEADKNDKSVKDLVILLYETALLSSGFSLEDPQTHANRIYRMIKLGLGIDEDDPTADDTSAAVTEEMPPLEG DDDTSRMEEVD P07900 3.6.4.10 Glucocorticoid receptor Homo sapiens 0.04119897299999999 1 Escherichia coli BL21(DE3) LEVO PKLCLVCSDEASGCHYGVLTCGSCKVFFKRAVEGQHNYLCAGRNDCIIDKIRRKNCPACRYRKCLQAGMNLEARKTKKKI KGIQQATTGVSQETSENPGNKTIVPATLPQLTPTLVSLLEVIEPEVLYAGYDSSVPDSTWRIMTTLNMLGGRQVIAAVKW AKAIPGFRNLHLDDQMTLLQYSWMSLMAFALGWRSYRQSSANLLCFAPDLIINEQRMTLPCMYDQCKHMLYVSSELHRLQ VSYEEYLCMKTLLLLSSVPKDGLKSQELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLLDSMHEVVENLLNYCF QTFLDKTMSIEFPEMLAEIITNQIPKYSNGNIKKLLFHQK P04150 Peptidyl-prolyl cis-trans isomerase FKBP4, N-terminally processed Homo sapiens 0.051876519999999995 1 Escherichia coli BL21(DE3) LEVO MTAEEMKATESGAQSAPLPMEGVDISPKQDEGVLKVIKREGTGTEMPMIGDRVFVHYTGWLLDGTKFDSSLDRKDKFSFD LGKGEVIKAWDIAIATMKVGEVCHITCKPEYAYGSAGSPPKIPPNATLVFEVELFEFKGEDLTEEEDGGIIRRIQTRGEG YAKPNEGAIVEVALEGYYKDKLFDQRELRFEIGEGENLDLPYGLERAIQRMEKGEHSIVYLKPSYAFGSVGKEKFQIPPN AELKYELHLKSFEKAKESWEMNSEEKLEQSTIVKERGTVYFKEGKYKQALLQYKKIVSWLEYESSFSNEEAQKAQALRLA SHLNLAMCHLKLQAFSAAIESCNKALELDSNNEKGLFRRGEAHLAVNDFELARADFQKVLQLYPNNKAAKTQLAVCQQRI RRQLAREKKLYANMFERLAEEENKAKAEASSGDHPTDTEMKEEQKSNTAGSQSQVETEA Q02790 ADENOSINE-5'-TRIPHOSPHATE 0.000507181 2 ATP MAGNESIUM ION 2.4305e-05 2 MG DEXAMETHASONE 0.000392461 1 DEX singleParticle particle 7.5 Quantifoil R1.2/1.3 COPPER 400 CARBON HOLEY ETHANE 100 283 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.8 2.0 105000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GIF Bioquantum 20 GATAN K3 BIOQUANTUM (6k x 4k) 1 11162 5.9 67.0 1 7KRJ 3.01 FSC 0.143 CUT-OFF cryoSPARC 3.3.2 307109 MAXIMUM LIKELIHOOD RELION 3.0.8 MAXIMUM LIKELIHOOD RELION 3.0.8 RELION 3.0.8 emd_29068.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 267.19998 267.19998 267.19998 90.0 90.0 90.0 X Y Z -0.49168167 2.19702 0.0023564885 0.040498532 0.8349999 0.8349999 0.8349999 0.2 AUTHOR Sharpened consensus GR-Hsp90-FKBP52 map 5FWK A PDB experimental model 5FWK B PDB experimental model 1M2Z A PDB experimental model 1EJF A PDB experimental model emd_29068_msk_1.map emd_29068_msk_2.map emd_29068_msk_3.map emd_29068_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 267.19998 267.19998 267.19998 90.0 90.0 90.0 X Y Z -0.33727768 1.5791869 0.0024372796 0.036850456 0.8349999 0.8349999 0.8349999 AUTHOR Unsharpened consensus GR-Hsp90-FKBP52 map emd_29068_additional_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 267.19998 267.19998 267.19998 90.0 90.0 90.0 X Y Z -3.1543262 8.255349000000001 0.0024002527 0.09719392 0.8349999 0.8349999 0.8349999 AUTHOR Focused map on Hsp90 in the GR-Hsp90-FKBP52 complex emd_29068_additional_3.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 267.19998 267.19998 267.19998 90.0 90.0 90.0 X Y Z -1.5363111 2.8024707 0.0012562155 0.043844476 0.8349999 0.8349999 0.8349999 AUTHOR Focused map on GR-FKBP52 in the GR-Hsp90-FKBP52 complex emd_29068_additional_4.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 267.19998 267.19998 267.19998 90.0 90.0 90.0 X Y Z -0.45884725 8.255349000000001 0.021646114 0.0867179 0.8349999 0.8349999 0.8349999 AUTHOR Composite map for GR-Hsp90-FKBP52 with combined focused maps on Hsp90 and GR-FKBP52 emd_29068_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 267.19998 267.19998 267.19998 90.0 90.0 90.0 X Y Z -0.3577869 1.7493443 0.002689967 0.04251807 0.8349999 0.8349999 0.8349999 AUTHOR GR-Hsp90-FKBP52 Half Map 1 emd_29068_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 267.19998 267.19998 267.19998 90.0 90.0 90.0 X Y Z -0.42668098 1.780149 0.0027046846 0.042488713 0.8349999 0.8349999 0.8349999 AUTHOR GR-Hsp90-FKBP52 Half Map 2