REL PDBe PDBe PDBe 2015-05-08 2015-06-17 2015-07-29 2015-09-09 Structure of adenovirus light particle Ad5/FC31 L2 Condezo GN Marabini R Ayora S Carazo JM Chillon M San Martin C icosahedral virus assembly, adenovirus, virus maturation Condezo GN Marabini R Ayora S Carazo JM Chillon M San Martin C Structures of adenovirus incomplete particles clarify capsid architecture and show maturation changes of packaging protein L1 52/55k. J.VIROL. 89 9653 9664 2015 26178997 doi:10.1128/JVI.01453-15 light density particle (called L2) produced by adenovirus delayed packaging mutant Ad5/FC31 light density particle (called L2) produced by adenovirus delayed packaging mutant Ad5/FC31 icosahedral virus capsid 1 125 Human adenovirus 5 Human adenovirus 5 Homo sapiens VERTEBRATES 150 950 25 VIRUS-LIKE PARTICLE SEROTYPE false true 5 singleParticle particle 0.03 7.8
20 mm Hepes, 150 mm NaCl
Quantifoil R2/4 300 mesh Cu/Rh glow discharged grids
 ETHANE LEICA EM CPC FEI TECNAI F20 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2.26 0.5 4.0 50000.0 GATAN LIQUID NITROGEN 2013-11-11 FEI EAGLE (4k x 4k) 14 889 12
CTF detection, phase flip, particle selection, extraction and normalization with Xmipp. 2D and 3D classification, 3D refinement and postprocessing with Relion.
micrograph
 I OTHER 12.3 OTHER Xmipp, Relion 6743 emd_3003.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 1075.2 1075.2 1075.2 90.0 90.0 90.0 X Y Z -1.81644154 4.99485445 0.0 1.0 4.2 4.2 4.2 1.4 AUTHOR Reconstruction of one type of light density particle (called L2) produced by adenovirus delayed packaging mutant Ad5/FC31
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 3iYN RIGID BODY FIT Chimera cross-correlation REAL