2024-04-10 REL PDBc PDBj PDBc 2023-05-01 2024-03-06 2024-03-06 2024-04-10 Ministry of Science and Technology (MoST, China) Nos.2018YFA0902000 China Ministry of Science and Technology (MoST, China) ZX-2021ZD0203404 China National Natural Science Foundation of China (NSFC) Nos. 32271330 China National Natural Science Foundation of China (NSFC) Nos. 32000889 China National Natural Science Foundation of China (NSFC) 31970547 China SPARTA monomer bound with guide-target, state 2 Li ZX Guo LJ Huang PP Xiao YB Chen MR SPARTA, Ago, Tir, DNA BINDING PROTEIN/DNA/RNA, DNA BINDING PROTEIN-DNA-RNA complex Guo L Huang P Li Z Shin YC Yan P Lu M Chen M Xiao Y Auto-inhibition and activation of a short Argonaute-associated TIR-APAZ defense system. Nat.Chem.Biol. US 20 512 520 2024 37932527 doi:10.1038/s41589-023-01478-0 1552-4469 8j8h FULLOVERLAP Short ago complexed with TIR-APAZ Short ago complexed with TIR-APAZ 0 3 4 1 2 Thermoflavifilum thermophilum Piwi domain-containing protein Thermoflavifilum thermophilum 0.061857792999999994 1 Escherichia coli BL21 LEVO MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMKELIYIEEPSILFAHGQKCTDPRDGLALFGPLNQIYGIKSGVVGT QKGLQIFKSYLDKIQKPIYNHNNITRPMFPGFEAVFGCKWESQNIVFKEITDEEIRRYLFNASTHKRTYDLVTLFNDKII TANKNDEERVDVWFVIVPEEIYKYCRPNSVLPNELVQTKSLISKSKAKSFRYTPTLFEEFNKKLKEVEKEAKTYNYDAQF HDQLKARLLEHTIPTQILRESTLAWRDFKNTFGAPIRDFSKIEGHLAWTISTAAYYKAGGKPWKLGDIRPGVCYLGLVYK KIEKSKNPQNACCAAQMFLDNGDGTVFKGEVGPWYNPEKGEYHLKPKEAKALLTQALESYKEQNKSYPKEVFIHARTRFN DEEWNAFNEVTPKNTNLVGVTITKSKPLKLYKTEGAFPIMRGNAYIVDEKKAFLWTLGFVPKLQSTLSMEVPNPIFIEIN KGEAEIQQVLKDILALTKLNYNACIYADGEPVTLRFANKIGEILTASTEIKTPPLAFKYYI A0A1I7NFD7 TIR domain-containing protein Thermoflavifilum thermophilum 0.056809667999999994 1 Escherichia coli BL21 LEVO MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSMRNKIFISHATPEDDDFTRWLSLKLIGLGYEVWCDILFLDKGVDFW STIEKEIRENTCKFLIVSSTAGNKREGVLKELAVATKVKKHLQDDMFIIPLAIDENLSYDDINIEIVRLNAIDFKKSWAK GLQDLLDAFEKQNVPKKPPDHSKSNLLYQQIFLHDKQAIEKEETYDSNWFPIISFPNELRFHRYDWRLPKQFDVRTLAFP AIRYKEYLCTFAWEYDFIHQLPKTETYNGQESIRISTSDILSGRYDTDFIRNYECQRLIVQLINKAFELRMKDKNVREYQ MSKTFAYWIEKGKLEKDKFEKIKLVGKQKNKYWHFGISAAGKLYPSPVLMVSSHIIFTMDGINLIKSKSIQHSSRRKQGK NWWNDKWREKLLAFIRFLSDDQNAIYLNVGSEEKILISNKPLKFFGKMSYVTPSEVTLEEESVLADINNFEEDTEDLDEL EDIE A0A1I7NFG5 RNA (5'-R(P*UP*GP*AP*CP*GP*GP*CP*UP*CP*UP*AP*AP*UP*CP*UP*AP*UP*UP*AP*GP*U)-3') Thermoflavifilum thermophilum 0.006651948999999999 1 UGACGGCUCUAAUCUAUUAGU DNA (25-MER) Thermoflavifilum thermophilum 0.0076749999999999995 1 (DC)(DA)(DA)(DC)(DT)(DA)(DA)(DT)(DA)(DG)(DA)(DT)(DT)(DA)(DG)(DA)(DG)(DC)(DC)(DG) (DT)(DC)(DA)(DA)(DT) DNA MAGNESIUM ION 2.4305e-05 1 MG singleParticle particle 7.5 ETHANE FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 1.0 2.4 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) COUNTING 40.0 1 3.4 FSC 0.143 CUT-OFF 117819 RANDOM ASSIGNMENT RANDOM ASSIGNMENT emd_36070.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 268.8 268.8 268.8 90.0 90.0 90.0 X Y Z -0.3645371 0.71136135 -0.000052798692 0.01575059 1.05 1.05 1.05 0.06 AUTHOR AB INITIO MODEL REAL emd_36070_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 268.8 268.8 268.8 90.0 90.0 90.0 X Y Z -0.4715612 0.87049925 0.00020509317 0.047206905 1.05 1.05 1.05 AUTHOR emd_36070_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 256 256 256 0 0 0 256 256 256 268.8 268.8 268.8 90.0 90.0 90.0 X Y Z -0.5219966 0.8179968 0.00022843863 0.04763533 1.05 1.05 1.05 AUTHOR