<?xml version="1.0" encoding="UTF-8"?>
<emd emdb_id="EMD-36498">
    <admin>
        <current_status>
            <date>2023-07-05</date>
            <code>REL</code>
            <processing_site>PDBj</processing_site>
        </current_status>
        <sites>
            <deposition>PDBj</deposition>
            <last_processing>PDBj</last_processing>
        </sites>
        <key_dates>
            <deposition>2023-06-13</deposition>
            <header_release>2023-07-05</header_release>
            <map_release>2023-07-05</map_release>
            <update>2023-07-05</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>National Natural Science Foundation of China (NSFC)</funding_body>
                <code>32271261</code>
                <country>China</country>
            </grant_reference>
        </grant_support>
        <title>Overall consensus map of the mouse sperm doublet microtubule</title>
        <authors_list>
            <author>Zhou LN</author>
            <author>Gui M</author>
            <author>Wu JP</author>
        </authors_list>
        <keywords>doublet microtubule, STRUCTURAL PROTEIN</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Zhou L</author>
                    <author order="2">Liu H</author>
                    <author order="3">Liu S</author>
                    <author order="4">Yang X</author>
                    <author order="5">Dong Y</author>
                    <author order="6">Pan Y</author>
                    <author order="7">Xiao Z</author>
                    <author order="8">Zheng B</author>
                    <author order="9">Sun Y</author>
                    <author order="10">Huang P</author>
                    <author order="11">Zhang X</author>
                    <author order="12">Hu J</author>
                    <author order="13">Sun R</author>
                    <author order="14">Feng S</author>
                    <author order="15">Zhu Y</author>
                    <author order="16">Liu M</author>
                    <author order="17">Gui M</author>
                    <author order="18">Wu J</author>
                    <title>Structures of sperm flagellar doublet microtubules expand the genetic spectrum of male infertility.</title>
                    <journal_abbreviation>Cell</journal_abbreviation>
                    <volume>186</volume>
                    <first_page>2897</first_page>
                    <last_page>2910.e19</last_page>
                    <year>2023</year>
                    <external_references type="PUBMED">37295417</external_references>
                    <external_references type="DOI">doi:10.1016/j.cell.2023.05.009</external_references>
                    <external_references type="ISSN">1097-4172</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <emdb_list>
            <emdb_reference>
                <emdb_id>EMD-36498</emdb_id>
                <relationship>
                    <other>associated EM volume</other>
                </relationship>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-35823</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
                <details>composite map</details>
            </emdb_reference>
        </emdb_list>
    </crossreferences>
    <sample>
        <name>Mouse sperm DMT</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Mouse sperm DMT</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>4</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>5</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>6</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>7</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>8</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>9</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>10</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>11</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>12</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>13</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>14</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>15</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>16</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>17</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>18</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>19</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>20</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>21</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>22</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>23</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>24</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>25</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>26</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>27</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>28</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>29</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>30</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>31</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>32</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>33</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>34</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>35</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>36</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>37</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>38</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>39</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>40</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>41</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>42</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>43</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>44</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>45</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>46</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>47</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>48</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>49</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="10090">Mus musculus</organism>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>filament</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <buffer>
                        <ph>7.5</ph>
                    </buffer>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <instrument>FEI VITROBOT MARK IV</instrument>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <nominal_defocus_min units="µm">1.5</nominal_defocus_min>
                    <nominal_defocus_max units="µm">2.6</nominal_defocus_max>
                    <nominal_magnification>81000.0</nominal_magnification>
                    <specimen_holder_model>FEI TITAN KRIOS AUTOGRID HOLDER</specimen_holder_model>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K3 (6k x 4k)</film_or_detector_model>
                            <number_real_images>25135</number_real_images>
                            <average_exposure_time units="s">2.56</average_exposure_time>
                            <average_electron_dose_per_image units="e/Å^2">50.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <startup_model type_of_model="EMDB MAP">
                    <emdb_id>EMD-24664</emdb_id>
                </startup_model>
                <final_reconstruction>
                    <resolution units="Å" res_type="BY AUTHOR">3.52</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <number_images_used>95290</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>NOT APPLICABLE</type>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>NOT APPLICABLE</type>
                </final_angle_assignment>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="2048001">
        <file>emd_36498.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>800</col>
            <row>800</row>
            <sec>800</sec>
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        <spacing>
            <x>800</x>
            <y>800</y>
            <z>800</z>
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        <cell>
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            <b units="Å">869.60004</b>
            <c units="Å">869.60004</c>
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            <beta units="deg">90.0</beta>
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            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
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        <statistics>
            <minimum>-0.90757704</minimum>
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            <average>0.005086698</average>
            <std>0.10199979</std>
        </statistics>
        <pixel_spacing>
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            <y units="Å">1.087</y>
            <z units="Å">1.087</z>
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        <contour_list>
            <contour primary="true">
                <level>0.485</level>
                <source>AUTHOR</source>
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        </contour_list>
        <label>::::EMDATABANK.org::::EMD-36498::::</label>
        <annotation_details>Consensus map of the mouse sperm doublet microtubule</annotation_details>
    </map>
    <interpretation>
        <half_map_list>
            <half_map format="CCP4" size_kbytes="2048001">
                <file>emd_36498_half_map_1.map.gz</file>
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                <dimensions>
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                    <row>800</row>
                    <sec>800</sec>
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                    <sec>0</sec>
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                <spacing>
                    <x>800</x>
                    <y>800</y>
                    <z>800</z>
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                    <a units="Å">869.60004</a>
                    <b units="Å">869.60004</b>
                    <c units="Å">869.60004</c>
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                    <gamma units="deg">90.0</gamma>
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                    <medium>Y</medium>
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                    <std>0.24447528</std>
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                    <y units="Å">1.087</y>
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                <contour_list>
                    <contour primary="true">
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                <label>::::EMDATABANK.org::::EMD-36498::::</label>
            </half_map>
            <half_map format="CCP4" size_kbytes="2048001">
                <file>emd_36498_half_map_2.map.gz</file>
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                <dimensions>
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                    <sec>800</sec>
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                <statistics>
                    <minimum>-1.8319132</minimum>
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                <contour_list>
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                <label>::::EMDATABANK.org::::EMD-36498::::</label>
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        </half_map_list>
    </interpretation>
</emd>
