2024-05-29 REL PDBj PDBj PDBj 2023-07-04 2024-05-01 2024-05-01 2024-05-29 Chinese Academy of Sciences China Cryo-EM structure of RCD-1 pore from Neurospora crassa Hou YJ Sun Q Li Y Ding J Pyroptosis, Gasdermnin, Pore, Allorecognition, IMMUNE SYSTEM Li Y Hou Y Sun Q Zeng H Meng F Tian X He Q Shao F Ding J Cleavage-independent activation of ancient eukaryotic gasdermins and structural mechanisms. Science US 384 adm9190 adm9190 2024 38662913 doi:10.1126/science.adm9190 1095-9203 0038 SCIEAS 8jyz FULLOVERLAP RCD-1 pore from Neurospora crassa RCD-1 pore from Neurospora crassa 0 1 2 Neurospora crassa 0.62 Gasdermin-like protein rcd-1-1 Neurospora crassa 0.029218692999999997 11 Escherichia coli BL21(DE3) LEVO SGRPMDKCWFTLDNAHYPPPSLDSMRSGHPISPASLGHLIPSLAHLDQIINAKAIEPFPATMDIHGPTIIEDFKWDHSHE YSLSLGGKVPIPLAPAGVPFVDLNVGLGGAFSRSVANYWEFDRLERYIMQPTRSYVQKCIERDEVKRWIAKNKSMMMMGR WEVYMITGIIVARGGGRKKKEKTTGKEFSVEVTVEVPLIVEAGPGGKRNTARQKTWGTSQTGDFVWAVRLAKITKSGLHS DWKMETVFGKTSSFRGQKAIF Q7SBA0 Gasdermin-like protein rcd-1-2 Neurospora crassa 0.027630418 11 Escherichia coli BL21(DE3) LEVO SGRPMDNEEWFPLKQTHYPPPTIPSMKTGHPTGPISIGHIIPDLRHLDNVINCKGFEPFPPNMDVFTAHYEQCHFGDHLN SEFVVQAKAAAPIENIVPGVDVTGSAGLHHTNITSDRWEYDSVVEYAVYPTRQYIDRLLESKEVRQYIQKSKKLLGGWCV YMVTGIMVARGGGRNVTSEEKGAGVSGNVGFQVPGIGEFAPEVGWDTKTKTKVNAHHTTDFVCAIRLVKIAKSGLRSSWT MKKVTREF P0DW10 singleParticle particle 8.0 20.0 Tris 300.0 NaCl 0.5 TCEP 0.2 C12E8 Quantifoil R1.2/1.3 GOLD 300 GRAPHENE OXIDE GLOW DISCHARGE ETHANE 100 277 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 1.5 3.0 GATAN K3 (6k x 4k) 56.0 1 C11 3.63 FSC 0.143 CUT-OFF 118156 MAXIMUM LIKELIHOOD PROJECTION MATCHING emd_36734.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 380 380 380 0 0 0 380 380 380 406.6 406.6 406.6 90.0 90.0 90.0 X Y Z -2.7799306 4.042685 0.001525738 0.08611175 1.07 1.07 1.07 0.35 AUTHOR 8JYX PDB experimental model 8JYY PDB experimental model emd_36734_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 380 380 380 0 0 0 380 380 380 406.6 406.6 406.6 90.0 90.0 90.0 X Y Z -0.26924565 1.0444736 0.0015545158 0.048523996 1.07 1.07 1.07 AUTHOR emd_36734_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 380 380 380 0 0 0 380 380 380 406.6 406.6 406.6 90.0 90.0 90.0 X Y Z -0.20169605 1.0436025 0.00156405 0.0484965 1.07 1.07 1.07 AUTHOR